In vitro ion adsorption and cytocompatibility of dicalcium phosphate ceramics
Abstract Background In vitro cell testing of degradable bioceramics such as brushite or monetite is often challenging due to the ion release into or adsorption from the culture medium. These ionic changes are then mostly responsible for cell proliferation and activity, which prohibits the investigat...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
American Association for the Advancement of Science (AAAS)
2017-06-01
|
| Series: | Biomaterials Research |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s40824-017-0096-4 |
| _version_ | 1827337563019411456 |
|---|---|
| author | Martha Schamel Jake E. Barralet Jürgen Groll Uwe Gbureck |
| author_facet | Martha Schamel Jake E. Barralet Jürgen Groll Uwe Gbureck |
| author_sort | Martha Schamel |
| collection | DOAJ |
| description | Abstract Background In vitro cell testing of degradable bioceramics such as brushite or monetite is often challenging due to the ion release into or adsorption from the culture medium. These ionic changes are then mostly responsible for cell proliferation and activity, which prohibits the investigation of effects originating from surface topography or further material modifications. Methods Here, we aimed to solve this problem by developing a pre-conditioning regime following the repeated immersion of brushite and monetite samples in various Ca2+, Mg2+ and PO4 3− containing electrolytes, followed by studying ion adsorption / release as well as changes in phase composition and in vitro cytocompatibility with MG63 cells. Results The results demonstrated that by using DMEM cell culture medium in a ratio of 10 ml/sample was sufficient to minimize changes of ionic composition after 7 d with a daily change of the medium. This leads to changes of the surface composition with dissolution of the brushite phase. In turn, this also positively influences the in vitro cytocompatibility with a 2–3 fold higher cell number and cell activity on the DMEM pretreated surfaces. Conclusions Controlled sample washing prior to cell testing using DMEM medium seems to be a valuable procedure not only to stabilize the pH during cell culture but also to maintain ion concentrations within a cell friendly range. |
| first_indexed | 2024-03-07T18:57:58Z |
| format | Article |
| id | doaj.art-0b54d198bf134c64af4cf0024ab6e7e0 |
| institution | Directory Open Access Journal |
| issn | 2055-7124 |
| language | English |
| last_indexed | 2024-03-07T18:57:58Z |
| publishDate | 2017-06-01 |
| publisher | American Association for the Advancement of Science (AAAS) |
| record_format | Article |
| series | Biomaterials Research |
| spelling | doaj.art-0b54d198bf134c64af4cf0024ab6e7e02024-03-02T00:01:15ZengAmerican Association for the Advancement of Science (AAAS)Biomaterials Research2055-71242017-06-012111810.1186/s40824-017-0096-4In vitro ion adsorption and cytocompatibility of dicalcium phosphate ceramicsMartha Schamel0Jake E. Barralet1Jürgen Groll2Uwe Gbureck3Department of Functional Materials in Medicine and Dentistry, University of WürzburgDepartment of Surgery, Faculty of Medicine, Faculty of Dentistry, McGill UniversityDepartment of Functional Materials in Medicine and Dentistry, University of WürzburgDepartment of Functional Materials in Medicine and Dentistry, University of WürzburgAbstract Background In vitro cell testing of degradable bioceramics such as brushite or monetite is often challenging due to the ion release into or adsorption from the culture medium. These ionic changes are then mostly responsible for cell proliferation and activity, which prohibits the investigation of effects originating from surface topography or further material modifications. Methods Here, we aimed to solve this problem by developing a pre-conditioning regime following the repeated immersion of brushite and monetite samples in various Ca2+, Mg2+ and PO4 3− containing electrolytes, followed by studying ion adsorption / release as well as changes in phase composition and in vitro cytocompatibility with MG63 cells. Results The results demonstrated that by using DMEM cell culture medium in a ratio of 10 ml/sample was sufficient to minimize changes of ionic composition after 7 d with a daily change of the medium. This leads to changes of the surface composition with dissolution of the brushite phase. In turn, this also positively influences the in vitro cytocompatibility with a 2–3 fold higher cell number and cell activity on the DMEM pretreated surfaces. Conclusions Controlled sample washing prior to cell testing using DMEM medium seems to be a valuable procedure not only to stabilize the pH during cell culture but also to maintain ion concentrations within a cell friendly range.http://link.springer.com/article/10.1186/s40824-017-0096-4BrushiteMonetiteCell cultureIon adsorption |
| spellingShingle | Martha Schamel Jake E. Barralet Jürgen Groll Uwe Gbureck In vitro ion adsorption and cytocompatibility of dicalcium phosphate ceramics Biomaterials Research Brushite Monetite Cell culture Ion adsorption |
| title | In vitro ion adsorption and cytocompatibility of dicalcium phosphate ceramics |
| title_full | In vitro ion adsorption and cytocompatibility of dicalcium phosphate ceramics |
| title_fullStr | In vitro ion adsorption and cytocompatibility of dicalcium phosphate ceramics |
| title_full_unstemmed | In vitro ion adsorption and cytocompatibility of dicalcium phosphate ceramics |
| title_short | In vitro ion adsorption and cytocompatibility of dicalcium phosphate ceramics |
| title_sort | in vitro ion adsorption and cytocompatibility of dicalcium phosphate ceramics |
| topic | Brushite Monetite Cell culture Ion adsorption |
| url | http://link.springer.com/article/10.1186/s40824-017-0096-4 |
| work_keys_str_mv | AT marthaschamel invitroionadsorptionandcytocompatibilityofdicalciumphosphateceramics AT jakeebarralet invitroionadsorptionandcytocompatibilityofdicalciumphosphateceramics AT jurgengroll invitroionadsorptionandcytocompatibilityofdicalciumphosphateceramics AT uwegbureck invitroionadsorptionandcytocompatibilityofdicalciumphosphateceramics |