Investigation of multidrug-resistant ST2 Acinetobacter baumannii isolated from Saint George hospital in Lebanon
Abstract Background Acinetobacter baumannii is an opportunistic pathogen causing various nosocomial infections. The spread of multidrug-resistant A. baumannii is a major public health problem. The aim of this study was to investigate the molecular epidemiology and the genetic support of multidrug-re...
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BMC
2019-02-01
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| Series: | BMC Microbiology |
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| Online Access: | http://link.springer.com/article/10.1186/s12866-019-1401-2 |
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| author | Tania Nawfal Dagher Charbel Al-Bayssari Selma Chabou Nadine Antar Seydina M. Diene Eid Azar Jean-Marc Rolain |
| author_facet | Tania Nawfal Dagher Charbel Al-Bayssari Selma Chabou Nadine Antar Seydina M. Diene Eid Azar Jean-Marc Rolain |
| author_sort | Tania Nawfal Dagher |
| collection | DOAJ |
| description | Abstract Background Acinetobacter baumannii is an opportunistic pathogen causing various nosocomial infections. The spread of multidrug-resistant A. baumannii is a major public health problem. The aim of this study was to investigate the molecular epidemiology and the genetic support of multidrug-resistant A. baumannii isolates collected from Saint-Georges Hospital in Lebanon. Methods Between January and August 2016, 31 A. baumannii isolates were collected from sputum samples of patients infected with ventilator-associated pneumonia (VAP) and treated with colistin-carbapenem combination therapy. Antibiotic susceptibility testing was performed using the disk diffusion method. Carbapenemases, extended spectrum β-lactamases encoding genes and mcr-1/2 genes were investigated by RT-PCR and standard PCR. The epidemiological relatedness of the strains was studied using MLST analysis. Results Most of the isolates exhibited multidrug-resistant phenotypes. All the isolates were carbapenem-resistant and among them, 30 carried the class D carbapenemase bla oxa-23 gene while one isolate carried bla oxa-72 gene. MLST results revealed three sequence types, namely ST2, ST699, and ST627. Isolates having ST2 were the most prevalent clone (29/31, 93.5%). Conclusions This study shows a nosocomial spread of multidrug-resistant A. baumannii ST2 having bla OXA-23 gene in Saint-George in Lebanon. Monitoring and control measures need to be adopted to avoid the spread of A. baumannii to patients. |
| first_indexed | 2024-12-11T12:17:43Z |
| format | Article |
| id | doaj.art-0b5e14b0e0204baeac6499acc9243c12 |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-12-11T12:17:43Z |
| publishDate | 2019-02-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj.art-0b5e14b0e0204baeac6499acc9243c122022-12-22T01:07:37ZengBMCBMC Microbiology1471-21802019-02-011911710.1186/s12866-019-1401-2Investigation of multidrug-resistant ST2 Acinetobacter baumannii isolated from Saint George hospital in LebanonTania Nawfal Dagher0Charbel Al-Bayssari1Selma Chabou2Nadine Antar3Seydina M. Diene4Eid Azar5Jean-Marc Rolain6Aix Marseille Univ, IRD, APHM, MEPHI, IHU-Méditerranée Infection, Faculté de Médecine et de PharmacieFaculty of Sciences III, Lebanese UniversityAix Marseille Univ, IRD, APHM, MEPHI, IHU-Méditerranée Infection, Faculté de Médecine et de PharmacieSaint George Hospital University Medical Center, Faculty of Medicine and Medical Sciences, University of BalamandAix Marseille Univ, IRD, APHM, MEPHI, IHU-Méditerranée Infection, Faculté de Médecine et de PharmacieSaint George Hospital University Medical Center, Faculty of Medicine and Medical Sciences, University of BalamandAix Marseille Univ, IRD, APHM, MEPHI, IHU-Méditerranée Infection, Faculté de Médecine et de PharmacieAbstract Background Acinetobacter baumannii is an opportunistic pathogen causing various nosocomial infections. The spread of multidrug-resistant A. baumannii is a major public health problem. The aim of this study was to investigate the molecular epidemiology and the genetic support of multidrug-resistant A. baumannii isolates collected from Saint-Georges Hospital in Lebanon. Methods Between January and August 2016, 31 A. baumannii isolates were collected from sputum samples of patients infected with ventilator-associated pneumonia (VAP) and treated with colistin-carbapenem combination therapy. Antibiotic susceptibility testing was performed using the disk diffusion method. Carbapenemases, extended spectrum β-lactamases encoding genes and mcr-1/2 genes were investigated by RT-PCR and standard PCR. The epidemiological relatedness of the strains was studied using MLST analysis. Results Most of the isolates exhibited multidrug-resistant phenotypes. All the isolates were carbapenem-resistant and among them, 30 carried the class D carbapenemase bla oxa-23 gene while one isolate carried bla oxa-72 gene. MLST results revealed three sequence types, namely ST2, ST699, and ST627. Isolates having ST2 were the most prevalent clone (29/31, 93.5%). Conclusions This study shows a nosocomial spread of multidrug-resistant A. baumannii ST2 having bla OXA-23 gene in Saint-George in Lebanon. Monitoring and control measures need to be adopted to avoid the spread of A. baumannii to patients.http://link.springer.com/article/10.1186/s12866-019-1401-2Acinetobacter baumanniiOXA-23ST2 clone |
| spellingShingle | Tania Nawfal Dagher Charbel Al-Bayssari Selma Chabou Nadine Antar Seydina M. Diene Eid Azar Jean-Marc Rolain Investigation of multidrug-resistant ST2 Acinetobacter baumannii isolated from Saint George hospital in Lebanon BMC Microbiology Acinetobacter baumannii OXA-23 ST2 clone |
| title | Investigation of multidrug-resistant ST2 Acinetobacter baumannii isolated from Saint George hospital in Lebanon |
| title_full | Investigation of multidrug-resistant ST2 Acinetobacter baumannii isolated from Saint George hospital in Lebanon |
| title_fullStr | Investigation of multidrug-resistant ST2 Acinetobacter baumannii isolated from Saint George hospital in Lebanon |
| title_full_unstemmed | Investigation of multidrug-resistant ST2 Acinetobacter baumannii isolated from Saint George hospital in Lebanon |
| title_short | Investigation of multidrug-resistant ST2 Acinetobacter baumannii isolated from Saint George hospital in Lebanon |
| title_sort | investigation of multidrug resistant st2 acinetobacter baumannii isolated from saint george hospital in lebanon |
| topic | Acinetobacter baumannii OXA-23 ST2 clone |
| url | http://link.springer.com/article/10.1186/s12866-019-1401-2 |
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