OsCERK1 Contributes to Cupric Oxide Nanoparticles Induced Phytotoxicity and Basal Resistance against Blast by Regulating the Anti-Oxidant System in Rice
CuO NPs (cupric oxide nanoparticles) are widely used in various fields due to their high electrical conductivity, electronic correlation effect, and special physical property. Notably, CuO NPs have good application prospects in agricultural production because of its antifungal activity to prevent cr...
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| Format: | Article |
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MDPI AG
2022-12-01
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| Series: | Journal of Fungi |
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| Online Access: | https://www.mdpi.com/2309-608X/9/1/36 |
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| author | Ya Chen Zhiquan Liu Shuai Meng Zhenan Shen Huanbin Shi Jiehua Qiu Fucheng Lin Shu Zhang Yanjun Kou |
| author_facet | Ya Chen Zhiquan Liu Shuai Meng Zhenan Shen Huanbin Shi Jiehua Qiu Fucheng Lin Shu Zhang Yanjun Kou |
| author_sort | Ya Chen |
| collection | DOAJ |
| description | CuO NPs (cupric oxide nanoparticles) are widely used in various fields due to their high electrical conductivity, electronic correlation effect, and special physical property. Notably, CuO NPs have good application prospects in agricultural production because of its antifungal activity to prevent crop diseases. However, the increasing release of CuO NPs into the environment has resulted in a serious threat to the ecosystem, including plants. Previous studies have reported the toxicity of CuO NPs on rice, but little is known about the underlying molecular mechanisms or specific genes involved in the response to CuO NPs. In this study, we found that the rice well-known receptor Chitin Elicitor Receptor Kinase 1 (OsCERK1), which is essential for basal resistance against pathogens, is involved in CuO NPs stress in rice. Knockout of <i>OsCERK1</i> gene resulted in enhanced tolerance to CuO NPs stress. Furthermore, it was revealed that OsCERK1 reduces the tolerance to CuO NPs stress by regulating the anti-oxidant system and increasing the accumulation of H<sub>2</sub>O<sub>2</sub> in rice. In addition, CuO NPs treatment significantly enhances the basal resistance against <i>M. oryzae</i> which is mediated by OsCERK1. In conclusion, this study demonstrated a dual role of OsCERK1 in response to CuO NPs stress and <i>M. oryzae</i> infection by modulating ROS accumulation, which expands our understanding about the crosstalk between abiotic and biotic stresses. |
| first_indexed | 2024-03-09T12:04:20Z |
| format | Article |
| id | doaj.art-0b5fc77cdac646aca471dd43af2d9e3d |
| institution | Directory Open Access Journal |
| issn | 2309-608X |
| language | English |
| last_indexed | 2024-03-09T12:04:20Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
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| series | Journal of Fungi |
| spelling | doaj.art-0b5fc77cdac646aca471dd43af2d9e3d2023-11-30T22:59:27ZengMDPI AGJournal of Fungi2309-608X2022-12-01913610.3390/jof9010036OsCERK1 Contributes to Cupric Oxide Nanoparticles Induced Phytotoxicity and Basal Resistance against Blast by Regulating the Anti-Oxidant System in RiceYa Chen0Zhiquan Liu1Shuai Meng2Zhenan Shen3Huanbin Shi4Jiehua Qiu5Fucheng Lin6Shu Zhang7Yanjun Kou8State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 311400, ChinaState Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 311400, ChinaState Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 311400, ChinaState Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 311400, ChinaState Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 311400, ChinaState Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 311400, ChinaCollege of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, ChinaKey Laboratory of Integrated Pest Management on Crops in Central China, Ministry of Agriculture, Wuhan 430064, ChinaState Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 311400, ChinaCuO NPs (cupric oxide nanoparticles) are widely used in various fields due to their high electrical conductivity, electronic correlation effect, and special physical property. Notably, CuO NPs have good application prospects in agricultural production because of its antifungal activity to prevent crop diseases. However, the increasing release of CuO NPs into the environment has resulted in a serious threat to the ecosystem, including plants. Previous studies have reported the toxicity of CuO NPs on rice, but little is known about the underlying molecular mechanisms or specific genes involved in the response to CuO NPs. In this study, we found that the rice well-known receptor Chitin Elicitor Receptor Kinase 1 (OsCERK1), which is essential for basal resistance against pathogens, is involved in CuO NPs stress in rice. Knockout of <i>OsCERK1</i> gene resulted in enhanced tolerance to CuO NPs stress. Furthermore, it was revealed that OsCERK1 reduces the tolerance to CuO NPs stress by regulating the anti-oxidant system and increasing the accumulation of H<sub>2</sub>O<sub>2</sub> in rice. In addition, CuO NPs treatment significantly enhances the basal resistance against <i>M. oryzae</i> which is mediated by OsCERK1. In conclusion, this study demonstrated a dual role of OsCERK1 in response to CuO NPs stress and <i>M. oryzae</i> infection by modulating ROS accumulation, which expands our understanding about the crosstalk between abiotic and biotic stresses.https://www.mdpi.com/2309-608X/9/1/36<i>Oryza sativa</i><i>Magnaporthe oryzae</i>rice blastbasal resistancecupric oxide nanoparticlesROS |
| spellingShingle | Ya Chen Zhiquan Liu Shuai Meng Zhenan Shen Huanbin Shi Jiehua Qiu Fucheng Lin Shu Zhang Yanjun Kou OsCERK1 Contributes to Cupric Oxide Nanoparticles Induced Phytotoxicity and Basal Resistance against Blast by Regulating the Anti-Oxidant System in Rice Journal of Fungi <i>Oryza sativa</i> <i>Magnaporthe oryzae</i> rice blast basal resistance cupric oxide nanoparticles ROS |
| title | OsCERK1 Contributes to Cupric Oxide Nanoparticles Induced Phytotoxicity and Basal Resistance against Blast by Regulating the Anti-Oxidant System in Rice |
| title_full | OsCERK1 Contributes to Cupric Oxide Nanoparticles Induced Phytotoxicity and Basal Resistance against Blast by Regulating the Anti-Oxidant System in Rice |
| title_fullStr | OsCERK1 Contributes to Cupric Oxide Nanoparticles Induced Phytotoxicity and Basal Resistance against Blast by Regulating the Anti-Oxidant System in Rice |
| title_full_unstemmed | OsCERK1 Contributes to Cupric Oxide Nanoparticles Induced Phytotoxicity and Basal Resistance against Blast by Regulating the Anti-Oxidant System in Rice |
| title_short | OsCERK1 Contributes to Cupric Oxide Nanoparticles Induced Phytotoxicity and Basal Resistance against Blast by Regulating the Anti-Oxidant System in Rice |
| title_sort | oscerk1 contributes to cupric oxide nanoparticles induced phytotoxicity and basal resistance against blast by regulating the anti oxidant system in rice |
| topic | <i>Oryza sativa</i> <i>Magnaporthe oryzae</i> rice blast basal resistance cupric oxide nanoparticles ROS |
| url | https://www.mdpi.com/2309-608X/9/1/36 |
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