miR-150-5p inhibits hepatoma cell migration and invasion by targeting MMP14.
Hepatocellular carcinoma (HCC) is one of the leading causes of cancer-related mortality worldwide. Despite progress in diagnostics and treatment of HCC, its prognosis remains poor because the molecular mechanisms underlying hepatocarcinogenesis are not well understood. In the study, we focused on id...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4280173?pdf=render |
| _version_ | 1818449144748965888 |
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| author | Tao Li Junjie Xie Chuan Shen Dongfeng Cheng Yuan Shi Zhichong Wu Qian Zhan Xiaxing Deng Hao Chen Baiyong Shen Chenghong Peng Hongwei Li Zhecheng Zhu |
| author_facet | Tao Li Junjie Xie Chuan Shen Dongfeng Cheng Yuan Shi Zhichong Wu Qian Zhan Xiaxing Deng Hao Chen Baiyong Shen Chenghong Peng Hongwei Li Zhecheng Zhu |
| author_sort | Tao Li |
| collection | DOAJ |
| description | Hepatocellular carcinoma (HCC) is one of the leading causes of cancer-related mortality worldwide. Despite progress in diagnostics and treatment of HCC, its prognosis remains poor because the molecular mechanisms underlying hepatocarcinogenesis are not well understood. In the study, we focused on identifying the role of miRNAs in HCC progression. miRNA microarray was used to analyze the differentially expressed miRNAs, and the results were validated by qPCR. We found that the miR-150-5p expression is down-regulated in HCC tissues compared with pair non-tumor tissues. miR-150-5p expression is also decreased in metastatic cancer tissues compared with pair primary tissues, indicating that miR-150-5p may be involved in HCC metastasis. Functionally, miR-150-5p inhibition significantly promotes hepatoma cell migration and invasion, whereas miR-150-5p overexpression suppresses cancer cell migration and invasion in vitro. The matrix metalloproteinase 14 (MMP14) is identified as a new target gene of miR-150-5p. miR-150-5p markedly inhibits MMP14 expression in hepatoma cells, and miR-150-5p expression is negative correlation with MMP14 expression in vivo. More important, re-expression of MMP14 in hepatoma cells partially reverses the effect of miR-150-5p in inhibiting cell invasion. |
| first_indexed | 2024-12-14T20:30:45Z |
| format | Article |
| id | doaj.art-0b5ffec8f0f54ca19b4155d31052a8ed |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-14T20:30:45Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-0b5ffec8f0f54ca19b4155d31052a8ed2022-12-21T22:48:31ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-01912e11557710.1371/journal.pone.0115577miR-150-5p inhibits hepatoma cell migration and invasion by targeting MMP14.Tao LiJunjie XieChuan ShenDongfeng ChengYuan ShiZhichong WuQian ZhanXiaxing DengHao ChenBaiyong ShenChenghong PengHongwei LiZhecheng ZhuHepatocellular carcinoma (HCC) is one of the leading causes of cancer-related mortality worldwide. Despite progress in diagnostics and treatment of HCC, its prognosis remains poor because the molecular mechanisms underlying hepatocarcinogenesis are not well understood. In the study, we focused on identifying the role of miRNAs in HCC progression. miRNA microarray was used to analyze the differentially expressed miRNAs, and the results were validated by qPCR. We found that the miR-150-5p expression is down-regulated in HCC tissues compared with pair non-tumor tissues. miR-150-5p expression is also decreased in metastatic cancer tissues compared with pair primary tissues, indicating that miR-150-5p may be involved in HCC metastasis. Functionally, miR-150-5p inhibition significantly promotes hepatoma cell migration and invasion, whereas miR-150-5p overexpression suppresses cancer cell migration and invasion in vitro. The matrix metalloproteinase 14 (MMP14) is identified as a new target gene of miR-150-5p. miR-150-5p markedly inhibits MMP14 expression in hepatoma cells, and miR-150-5p expression is negative correlation with MMP14 expression in vivo. More important, re-expression of MMP14 in hepatoma cells partially reverses the effect of miR-150-5p in inhibiting cell invasion.http://europepmc.org/articles/PMC4280173?pdf=render |
| spellingShingle | Tao Li Junjie Xie Chuan Shen Dongfeng Cheng Yuan Shi Zhichong Wu Qian Zhan Xiaxing Deng Hao Chen Baiyong Shen Chenghong Peng Hongwei Li Zhecheng Zhu miR-150-5p inhibits hepatoma cell migration and invasion by targeting MMP14. PLoS ONE |
| title | miR-150-5p inhibits hepatoma cell migration and invasion by targeting MMP14. |
| title_full | miR-150-5p inhibits hepatoma cell migration and invasion by targeting MMP14. |
| title_fullStr | miR-150-5p inhibits hepatoma cell migration and invasion by targeting MMP14. |
| title_full_unstemmed | miR-150-5p inhibits hepatoma cell migration and invasion by targeting MMP14. |
| title_short | miR-150-5p inhibits hepatoma cell migration and invasion by targeting MMP14. |
| title_sort | mir 150 5p inhibits hepatoma cell migration and invasion by targeting mmp14 |
| url | http://europepmc.org/articles/PMC4280173?pdf=render |
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