Identification of a Novel Pleiotropic Transcriptional Regulator Involved in Sporulation and Secondary Metabolism Production in <i>Chaetomium globosum</i>
Chaetoglobosin A (CheA), a well-known macrocyclic alkaloid with prominently highly antimycotic, antiparasitic, and antitumor properties, is mainly produced by <i>Chaetomium globosum</i>. However, a limited understanding of the transcriptional regulation of CheA biosynthesis has hampered...
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MDPI AG
2022-11-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/23/14849 |
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| author | Shanshan Zhao Kai Zhang Congyu Lin Ming Cheng Jinzhu Song Xin Ru Zhengran Wang Wan Wang Qian Yang |
| author_facet | Shanshan Zhao Kai Zhang Congyu Lin Ming Cheng Jinzhu Song Xin Ru Zhengran Wang Wan Wang Qian Yang |
| author_sort | Shanshan Zhao |
| collection | DOAJ |
| description | Chaetoglobosin A (CheA), a well-known macrocyclic alkaloid with prominently highly antimycotic, antiparasitic, and antitumor properties, is mainly produced by <i>Chaetomium globosum</i>. However, a limited understanding of the transcriptional regulation of CheA biosynthesis has hampered its application and commercialization in agriculture and biomedicine. Here, a comprehensive study of the <i>CgXpp1</i> gene, which encodes a basic helix-loop-helix family regulator with a putative role in the regulation of fungal growth and CheA biosynthesis, was performed by employing <i>CgXpp1</i>-disruption and <i>CgXpp1</i>-complementation strategies in the biocontrol species <i>C. globosum</i>. The results suggest that the <i>CgXpp1</i> gene could be an indirect negative regulator in CheA production. Interestingly, knockout of <i>CgXpp1</i> considerably increased the transcription levels of key genes and related regulatory factors associated with the CheA biosynthetic. Disruption of <i>CgXpp1</i> led to a significant reduction in spore production and attenuation of cell development, which was consistent with metabolome analysis results. Taken together, an in-depth analysis of pleiotropic regulation influenced by transcription factors could provide insights into the unexplored metabolic mechanisms associated with primary and secondary metabolite production. |
| first_indexed | 2024-03-09T17:46:08Z |
| format | Article |
| id | doaj.art-0b638b43d91048acb63025dd5e101e52 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T17:46:08Z |
| publishDate | 2022-11-01 |
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| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-0b638b43d91048acb63025dd5e101e522023-11-24T11:09:00ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-11-0123231484910.3390/ijms232314849Identification of a Novel Pleiotropic Transcriptional Regulator Involved in Sporulation and Secondary Metabolism Production in <i>Chaetomium globosum</i>Shanshan Zhao0Kai Zhang1Congyu Lin2Ming Cheng3Jinzhu Song4Xin Ru5Zhengran Wang6Wan Wang7Qian Yang8School of Life Science and Technology, Harbin Institute of Technology, Harbin 150080, ChinaSchool of Life Science and Technology, Harbin Institute of Technology, Harbin 150080, ChinaSchool of Life Science and Technology, Harbin Institute of Technology, Harbin 150080, ChinaSchool of Life Science and Technology, Harbin Institute of Technology, Harbin 150080, ChinaSchool of Life Science and Technology, Harbin Institute of Technology, Harbin 150080, ChinaSchool of Life Science and Technology, Harbin Institute of Technology, Harbin 150080, ChinaSchool of Life Science and Technology, Harbin Institute of Technology, Harbin 150080, ChinaSchool of Life Science and Technology, Harbin Institute of Technology, Harbin 150080, ChinaSchool of Life Science and Technology, Harbin Institute of Technology, Harbin 150080, ChinaChaetoglobosin A (CheA), a well-known macrocyclic alkaloid with prominently highly antimycotic, antiparasitic, and antitumor properties, is mainly produced by <i>Chaetomium globosum</i>. However, a limited understanding of the transcriptional regulation of CheA biosynthesis has hampered its application and commercialization in agriculture and biomedicine. Here, a comprehensive study of the <i>CgXpp1</i> gene, which encodes a basic helix-loop-helix family regulator with a putative role in the regulation of fungal growth and CheA biosynthesis, was performed by employing <i>CgXpp1</i>-disruption and <i>CgXpp1</i>-complementation strategies in the biocontrol species <i>C. globosum</i>. The results suggest that the <i>CgXpp1</i> gene could be an indirect negative regulator in CheA production. Interestingly, knockout of <i>CgXpp1</i> considerably increased the transcription levels of key genes and related regulatory factors associated with the CheA biosynthetic. Disruption of <i>CgXpp1</i> led to a significant reduction in spore production and attenuation of cell development, which was consistent with metabolome analysis results. Taken together, an in-depth analysis of pleiotropic regulation influenced by transcription factors could provide insights into the unexplored metabolic mechanisms associated with primary and secondary metabolite production.https://www.mdpi.com/1422-0067/23/23/14849pleiotropic regulator<i>Chaetomium globosum</i>chaetoglobosin Asporulationmetabolome |
| spellingShingle | Shanshan Zhao Kai Zhang Congyu Lin Ming Cheng Jinzhu Song Xin Ru Zhengran Wang Wan Wang Qian Yang Identification of a Novel Pleiotropic Transcriptional Regulator Involved in Sporulation and Secondary Metabolism Production in <i>Chaetomium globosum</i> International Journal of Molecular Sciences pleiotropic regulator <i>Chaetomium globosum</i> chaetoglobosin A sporulation metabolome |
| title | Identification of a Novel Pleiotropic Transcriptional Regulator Involved in Sporulation and Secondary Metabolism Production in <i>Chaetomium globosum</i> |
| title_full | Identification of a Novel Pleiotropic Transcriptional Regulator Involved in Sporulation and Secondary Metabolism Production in <i>Chaetomium globosum</i> |
| title_fullStr | Identification of a Novel Pleiotropic Transcriptional Regulator Involved in Sporulation and Secondary Metabolism Production in <i>Chaetomium globosum</i> |
| title_full_unstemmed | Identification of a Novel Pleiotropic Transcriptional Regulator Involved in Sporulation and Secondary Metabolism Production in <i>Chaetomium globosum</i> |
| title_short | Identification of a Novel Pleiotropic Transcriptional Regulator Involved in Sporulation and Secondary Metabolism Production in <i>Chaetomium globosum</i> |
| title_sort | identification of a novel pleiotropic transcriptional regulator involved in sporulation and secondary metabolism production in i chaetomium globosum i |
| topic | pleiotropic regulator <i>Chaetomium globosum</i> chaetoglobosin A sporulation metabolome |
| url | https://www.mdpi.com/1422-0067/23/23/14849 |
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