Protocol for high-throughput single-cell patterning using a reusable ultrathin metal microstencil
Summary: Exploiting convenient strategies for single-cell preparation while maintaining a high throughput remains challenging. This protocol describes a simple workflow for high-throughput single-cell patterning using a reusable ultrathin metal microstencil (UTmS). We describe UTmS-chip design, fabr...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-03-01
|
| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166723000734 |
| _version_ | 1811166099805306880 |
|---|---|
| author | Qingqing Tian Kunming Xing Yongshu Liu Qian Wang Haonan Sun Ying-Nan Sun Shusheng Zhang |
| author_facet | Qingqing Tian Kunming Xing Yongshu Liu Qian Wang Haonan Sun Ying-Nan Sun Shusheng Zhang |
| author_sort | Qingqing Tian |
| collection | DOAJ |
| description | Summary: Exploiting convenient strategies for single-cell preparation while maintaining a high throughput remains challenging. This protocol describes a simple workflow for high-throughput single-cell patterning using a reusable ultrathin metal microstencil (UTmS). We describe UTmS-chip design, fabrication, and quality characterization. We then detail the preparation of flat substrates and chip assembly for single-cell patterning, followed by culturing of cells on a chip. Finally, we describe the evaluation of single-cell patterning and the downstream applications for studying single-cell calcium release and apoptosis.For complete details on the use and execution of this protocol, please refer to Song et al. (2021).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| first_indexed | 2024-04-10T15:47:03Z |
| format | Article |
| id | doaj.art-0b648a320fa94a81ac3499be436ad2f9 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-04-10T15:47:03Z |
| publishDate | 2023-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-0b648a320fa94a81ac3499be436ad2f92023-02-12T04:15:38ZengElsevierSTAR Protocols2666-16672023-03-0141102115Protocol for high-throughput single-cell patterning using a reusable ultrathin metal microstencilQingqing Tian0Kunming Xing1Yongshu Liu2Qian Wang3Haonan Sun4Ying-Nan Sun5Shusheng Zhang6Shandong Province Key Laboratory of Detection Technology for Tumor Makers, School of Medicine, Linyi University, Linyi, Shandong 276005, ChinaLinyi People’s Hospital, Linyi, Shandong 276100, P. R. ChinaShandong Province Key Laboratory of Detection Technology for Tumor Makers, School of Medicine, Linyi University, Linyi, Shandong 276005, ChinaShandong Province Key Laboratory of Detection Technology for Tumor Makers, School of Medicine, Linyi University, Linyi, Shandong 276005, ChinaShandong Province Key Laboratory of Detection Technology for Tumor Makers, School of Medicine, Linyi University, Linyi, Shandong 276005, ChinaShandong Province Key Laboratory of Detection Technology for Tumor Makers, School of Medicine, Linyi University, Linyi, Shandong 276005, China; Corresponding authorShandong Province Key Laboratory of Detection Technology for Tumor Makers, School of Medicine, Linyi University, Linyi, Shandong 276005, China; Corresponding authorSummary: Exploiting convenient strategies for single-cell preparation while maintaining a high throughput remains challenging. This protocol describes a simple workflow for high-throughput single-cell patterning using a reusable ultrathin metal microstencil (UTmS). We describe UTmS-chip design, fabrication, and quality characterization. We then detail the preparation of flat substrates and chip assembly for single-cell patterning, followed by culturing of cells on a chip. Finally, we describe the evaluation of single-cell patterning and the downstream applications for studying single-cell calcium release and apoptosis.For complete details on the use and execution of this protocol, please refer to Song et al. (2021).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166723000734Cell BiologyCell cultureCell isolationSingle CellHigh Throughput ScreeningBiotechnology and bioengineering |
| spellingShingle | Qingqing Tian Kunming Xing Yongshu Liu Qian Wang Haonan Sun Ying-Nan Sun Shusheng Zhang Protocol for high-throughput single-cell patterning using a reusable ultrathin metal microstencil STAR Protocols Cell Biology Cell culture Cell isolation Single Cell High Throughput Screening Biotechnology and bioengineering |
| title | Protocol for high-throughput single-cell patterning using a reusable ultrathin metal microstencil |
| title_full | Protocol for high-throughput single-cell patterning using a reusable ultrathin metal microstencil |
| title_fullStr | Protocol for high-throughput single-cell patterning using a reusable ultrathin metal microstencil |
| title_full_unstemmed | Protocol for high-throughput single-cell patterning using a reusable ultrathin metal microstencil |
| title_short | Protocol for high-throughput single-cell patterning using a reusable ultrathin metal microstencil |
| title_sort | protocol for high throughput single cell patterning using a reusable ultrathin metal microstencil |
| topic | Cell Biology Cell culture Cell isolation Single Cell High Throughput Screening Biotechnology and bioengineering |
| url | http://www.sciencedirect.com/science/article/pii/S2666166723000734 |
| work_keys_str_mv | AT qingqingtian protocolforhighthroughputsinglecellpatterningusingareusableultrathinmetalmicrostencil AT kunmingxing protocolforhighthroughputsinglecellpatterningusingareusableultrathinmetalmicrostencil AT yongshuliu protocolforhighthroughputsinglecellpatterningusingareusableultrathinmetalmicrostencil AT qianwang protocolforhighthroughputsinglecellpatterningusingareusableultrathinmetalmicrostencil AT haonansun protocolforhighthroughputsinglecellpatterningusingareusableultrathinmetalmicrostencil AT yingnansun protocolforhighthroughputsinglecellpatterningusingareusableultrathinmetalmicrostencil AT shushengzhang protocolforhighthroughputsinglecellpatterningusingareusableultrathinmetalmicrostencil |