Reproductive Outcomes and Endocrine Profile in Artificially Inseminated versus Embryo Transferred Cows
The increasing use of in vitro embryo production (IVP) followed by embryo transfer (ET), alongside with cryopreservation of embryos, has risen concerns regarding the possible altered pregnancy rates, calving or even neonatal mortality. One of the hypotheses for these alterations is the current cultu...
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MDPI AG
2020-08-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/10/8/1359 |
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| author | Jordana S. Lopes Estefanía Alcázar-Triviño Cristina Soriano-Úbeda Meriem Hamdi Sebastian Cánovas Dimitrios Rizos Pilar Coy |
| author_facet | Jordana S. Lopes Estefanía Alcázar-Triviño Cristina Soriano-Úbeda Meriem Hamdi Sebastian Cánovas Dimitrios Rizos Pilar Coy |
| author_sort | Jordana S. Lopes |
| collection | DOAJ |
| description | The increasing use of in vitro embryo production (IVP) followed by embryo transfer (ET), alongside with cryopreservation of embryos, has risen concerns regarding the possible altered pregnancy rates, calving or even neonatal mortality. One of the hypotheses for these alterations is the current culture conditions of the IVP. In an attempt to better mimic the physiological milieu, embryos were produced with female reproductive fluids (RF) as supplements to culture medium, and another group of embryos were supplemented with bovine serum albumin (BSA) as in vitro control. Embryos were cryopreserved and transferred while, in parallel, an in vivo control (artificial insemination, AI) with the same bull used for IVP was included. An overview on pregnancy rates, recipients’ hormonal levels, parturition, and resulting calves were recorded. Results show much similarity between groups in terms of pregnancy rates, gestation length and calves’ weight. Nonetheless, several differences on hormonal levels were noted between recipients carrying AI embryos especially when compared to BSA. Some calving issues and neonatal mortality were observed in both IVP groups. In conclusion, most of the parameters studied were similar between both types of IVP derived embryos and the in vivo-derived embryos, suggesting that the IVP technology used was efficient enough for the safe production of calves. |
| first_indexed | 2024-03-10T17:53:28Z |
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| id | doaj.art-0b64a08ef9244f54b1e8e0f8df7c00fb |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T17:53:28Z |
| publishDate | 2020-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-0b64a08ef9244f54b1e8e0f8df7c00fb2023-11-20T09:15:05ZengMDPI AGAnimals2076-26152020-08-01108135910.3390/ani10081359Reproductive Outcomes and Endocrine Profile in Artificially Inseminated versus Embryo Transferred CowsJordana S. Lopes0Estefanía Alcázar-Triviño1Cristina Soriano-Úbeda2Meriem Hamdi3Sebastian Cánovas4Dimitrios Rizos5Pilar Coy6Physiology of Reproduction Group, Departamento de Fisiología, Facultad de Veterinaria, Universidad de Murcia, Campus Mare Nostrum, 30100 Murcia, SpainEl Barranquillo S.L., Torre Pacheco, 30700 Murcia, SpainPhysiology of Reproduction Group, Departamento de Fisiología, Facultad de Veterinaria, Universidad de Murcia, Campus Mare Nostrum, 30100 Murcia, SpainDepartment of Animal Reproduction, National Institute for Agriculture and Food Research and Technology (INIA), 28040 Madrid, SpainPhysiology of Reproduction Group, Departamento de Fisiología, Facultad de Veterinaria, Universidad de Murcia, Campus Mare Nostrum, 30100 Murcia, SpainDepartment of Animal Reproduction, National Institute for Agriculture and Food Research and Technology (INIA), 28040 Madrid, SpainPhysiology of Reproduction Group, Departamento de Fisiología, Facultad de Veterinaria, Universidad de Murcia, Campus Mare Nostrum, 30100 Murcia, SpainThe increasing use of in vitro embryo production (IVP) followed by embryo transfer (ET), alongside with cryopreservation of embryos, has risen concerns regarding the possible altered pregnancy rates, calving or even neonatal mortality. One of the hypotheses for these alterations is the current culture conditions of the IVP. In an attempt to better mimic the physiological milieu, embryos were produced with female reproductive fluids (RF) as supplements to culture medium, and another group of embryos were supplemented with bovine serum albumin (BSA) as in vitro control. Embryos were cryopreserved and transferred while, in parallel, an in vivo control (artificial insemination, AI) with the same bull used for IVP was included. An overview on pregnancy rates, recipients’ hormonal levels, parturition, and resulting calves were recorded. Results show much similarity between groups in terms of pregnancy rates, gestation length and calves’ weight. Nonetheless, several differences on hormonal levels were noted between recipients carrying AI embryos especially when compared to BSA. Some calving issues and neonatal mortality were observed in both IVP groups. In conclusion, most of the parameters studied were similar between both types of IVP derived embryos and the in vivo-derived embryos, suggesting that the IVP technology used was efficient enough for the safe production of calves.https://www.mdpi.com/2076-2615/10/8/1359embryo transferreproductive fluidspregnancyvitrificationcalving |
| spellingShingle | Jordana S. Lopes Estefanía Alcázar-Triviño Cristina Soriano-Úbeda Meriem Hamdi Sebastian Cánovas Dimitrios Rizos Pilar Coy Reproductive Outcomes and Endocrine Profile in Artificially Inseminated versus Embryo Transferred Cows Animals embryo transfer reproductive fluids pregnancy vitrification calving |
| title | Reproductive Outcomes and Endocrine Profile in Artificially Inseminated versus Embryo Transferred Cows |
| title_full | Reproductive Outcomes and Endocrine Profile in Artificially Inseminated versus Embryo Transferred Cows |
| title_fullStr | Reproductive Outcomes and Endocrine Profile in Artificially Inseminated versus Embryo Transferred Cows |
| title_full_unstemmed | Reproductive Outcomes and Endocrine Profile in Artificially Inseminated versus Embryo Transferred Cows |
| title_short | Reproductive Outcomes and Endocrine Profile in Artificially Inseminated versus Embryo Transferred Cows |
| title_sort | reproductive outcomes and endocrine profile in artificially inseminated versus embryo transferred cows |
| topic | embryo transfer reproductive fluids pregnancy vitrification calving |
| url | https://www.mdpi.com/2076-2615/10/8/1359 |
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