Immunoreactivity and neutralization study of Chinese Bungarus multicinctus antivenin and lab-prepared anti-bungarotoxin antisera towards purified bungarotoxins and snake venoms.
Bungarus multicinctus is the most venomous snake distributed in China and neighboring countries of Myanmar, Laos, north Vietnam and Thailand. The high mortality rate of B. multicinctus envenomation is attributed to the lethal components of α-, β-, γ- and κ- bungarotoxins contained in the venom. Alth...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2020-11-01
|
| Series: | PLoS Neglected Tropical Diseases |
| Online Access: | https://doi.org/10.1371/journal.pntd.0008873 |
| _version_ | 1818729903637397504 |
|---|---|
| author | Bo Lin Jia-Rui Zhang Hui-Juan Lu Lin Zhao Jing Chen Hong-Fei Zhang Xue-Song Wei Liang-Yu Zhang Xiao-Bing Wu Wen-Hui Lee |
| author_facet | Bo Lin Jia-Rui Zhang Hui-Juan Lu Lin Zhao Jing Chen Hong-Fei Zhang Xue-Song Wei Liang-Yu Zhang Xiao-Bing Wu Wen-Hui Lee |
| author_sort | Bo Lin |
| collection | DOAJ |
| description | Bungarus multicinctus is the most venomous snake distributed in China and neighboring countries of Myanmar, Laos, north Vietnam and Thailand. The high mortality rate of B. multicinctus envenomation is attributed to the lethal components of α-, β-, γ- and κ- bungarotoxins contained in the venom. Although anti-B. multicinctus sera were produced in Shanghai, Taiwan and Vietnam, the most widely clinic used product was term as B. multicinctus antivenin and manufactured by Shanghai Serum Bio-technology Co. Ltd. In the present investigation, high purity α-, β- and γ-bungarotoxins were separately isolated from B. multicinctus crude venom. Rabbit anti- α-, β- and γ-bungarotoxin antisera were prepared by common methods, respectively. LD50 values of α-, β- and γ-bungarotoxins were systematically determined via three administration pathways (intraperitoneal, intramuscular and intravenous injections) in Kunming mice. LD50 values of β-bungarotoxin were closely related with injection routines but those of both α- and γ-bungarotoxins were not dependent on the injection routines. Commercial B. multicinctus antivenin showed strong immunoreaction with high molecular weight fractions of the B. multicinctus but weakly recognized low molecular weight fractions like α- and γ-bungarotoxins. Although B. multicinctus antivenin showed immunoreaction with high molecular weight fractions of Bungarus fasciatus, Naja atra, Ophiophagus hannah venoms but the antivenin only demonstrated animal protection efficacy against O. hannah venom. These results indicated that the high molecular weight fractions of the O. hannah played an important role in venom lethality but those of B. fasciatus and N. atra did not have such a role. |
| first_indexed | 2024-12-17T22:53:17Z |
| format | Article |
| id | doaj.art-0b6655995c454c729a4785356c3ccdb0 |
| institution | Directory Open Access Journal |
| issn | 1935-2727 1935-2735 |
| language | English |
| last_indexed | 2024-12-17T22:53:17Z |
| publishDate | 2020-11-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Neglected Tropical Diseases |
| spelling | doaj.art-0b6655995c454c729a4785356c3ccdb02022-12-21T21:29:37ZengPublic Library of Science (PLoS)PLoS Neglected Tropical Diseases1935-27271935-27352020-11-011411e000887310.1371/journal.pntd.0008873Immunoreactivity and neutralization study of Chinese Bungarus multicinctus antivenin and lab-prepared anti-bungarotoxin antisera towards purified bungarotoxins and snake venoms.Bo LinJia-Rui ZhangHui-Juan LuLin ZhaoJing ChenHong-Fei ZhangXue-Song WeiLiang-Yu ZhangXiao-Bing WuWen-Hui LeeBungarus multicinctus is the most venomous snake distributed in China and neighboring countries of Myanmar, Laos, north Vietnam and Thailand. The high mortality rate of B. multicinctus envenomation is attributed to the lethal components of α-, β-, γ- and κ- bungarotoxins contained in the venom. Although anti-B. multicinctus sera were produced in Shanghai, Taiwan and Vietnam, the most widely clinic used product was term as B. multicinctus antivenin and manufactured by Shanghai Serum Bio-technology Co. Ltd. In the present investigation, high purity α-, β- and γ-bungarotoxins were separately isolated from B. multicinctus crude venom. Rabbit anti- α-, β- and γ-bungarotoxin antisera were prepared by common methods, respectively. LD50 values of α-, β- and γ-bungarotoxins were systematically determined via three administration pathways (intraperitoneal, intramuscular and intravenous injections) in Kunming mice. LD50 values of β-bungarotoxin were closely related with injection routines but those of both α- and γ-bungarotoxins were not dependent on the injection routines. Commercial B. multicinctus antivenin showed strong immunoreaction with high molecular weight fractions of the B. multicinctus but weakly recognized low molecular weight fractions like α- and γ-bungarotoxins. Although B. multicinctus antivenin showed immunoreaction with high molecular weight fractions of Bungarus fasciatus, Naja atra, Ophiophagus hannah venoms but the antivenin only demonstrated animal protection efficacy against O. hannah venom. These results indicated that the high molecular weight fractions of the O. hannah played an important role in venom lethality but those of B. fasciatus and N. atra did not have such a role.https://doi.org/10.1371/journal.pntd.0008873 |
| spellingShingle | Bo Lin Jia-Rui Zhang Hui-Juan Lu Lin Zhao Jing Chen Hong-Fei Zhang Xue-Song Wei Liang-Yu Zhang Xiao-Bing Wu Wen-Hui Lee Immunoreactivity and neutralization study of Chinese Bungarus multicinctus antivenin and lab-prepared anti-bungarotoxin antisera towards purified bungarotoxins and snake venoms. PLoS Neglected Tropical Diseases |
| title | Immunoreactivity and neutralization study of Chinese Bungarus multicinctus antivenin and lab-prepared anti-bungarotoxin antisera towards purified bungarotoxins and snake venoms. |
| title_full | Immunoreactivity and neutralization study of Chinese Bungarus multicinctus antivenin and lab-prepared anti-bungarotoxin antisera towards purified bungarotoxins and snake venoms. |
| title_fullStr | Immunoreactivity and neutralization study of Chinese Bungarus multicinctus antivenin and lab-prepared anti-bungarotoxin antisera towards purified bungarotoxins and snake venoms. |
| title_full_unstemmed | Immunoreactivity and neutralization study of Chinese Bungarus multicinctus antivenin and lab-prepared anti-bungarotoxin antisera towards purified bungarotoxins and snake venoms. |
| title_short | Immunoreactivity and neutralization study of Chinese Bungarus multicinctus antivenin and lab-prepared anti-bungarotoxin antisera towards purified bungarotoxins and snake venoms. |
| title_sort | immunoreactivity and neutralization study of chinese bungarus multicinctus antivenin and lab prepared anti bungarotoxin antisera towards purified bungarotoxins and snake venoms |
| url | https://doi.org/10.1371/journal.pntd.0008873 |
| work_keys_str_mv | AT bolin immunoreactivityandneutralizationstudyofchinesebungarusmulticinctusantiveninandlabpreparedantibungarotoxinantiseratowardspurifiedbungarotoxinsandsnakevenoms AT jiaruizhang immunoreactivityandneutralizationstudyofchinesebungarusmulticinctusantiveninandlabpreparedantibungarotoxinantiseratowardspurifiedbungarotoxinsandsnakevenoms AT huijuanlu immunoreactivityandneutralizationstudyofchinesebungarusmulticinctusantiveninandlabpreparedantibungarotoxinantiseratowardspurifiedbungarotoxinsandsnakevenoms AT linzhao immunoreactivityandneutralizationstudyofchinesebungarusmulticinctusantiveninandlabpreparedantibungarotoxinantiseratowardspurifiedbungarotoxinsandsnakevenoms AT jingchen immunoreactivityandneutralizationstudyofchinesebungarusmulticinctusantiveninandlabpreparedantibungarotoxinantiseratowardspurifiedbungarotoxinsandsnakevenoms AT hongfeizhang immunoreactivityandneutralizationstudyofchinesebungarusmulticinctusantiveninandlabpreparedantibungarotoxinantiseratowardspurifiedbungarotoxinsandsnakevenoms AT xuesongwei immunoreactivityandneutralizationstudyofchinesebungarusmulticinctusantiveninandlabpreparedantibungarotoxinantiseratowardspurifiedbungarotoxinsandsnakevenoms AT liangyuzhang immunoreactivityandneutralizationstudyofchinesebungarusmulticinctusantiveninandlabpreparedantibungarotoxinantiseratowardspurifiedbungarotoxinsandsnakevenoms AT xiaobingwu immunoreactivityandneutralizationstudyofchinesebungarusmulticinctusantiveninandlabpreparedantibungarotoxinantiseratowardspurifiedbungarotoxinsandsnakevenoms AT wenhuilee immunoreactivityandneutralizationstudyofchinesebungarusmulticinctusantiveninandlabpreparedantibungarotoxinantiseratowardspurifiedbungarotoxinsandsnakevenoms |