Evaluation of the recombinant egagb8/2 antigen for the diagnosis of cystic echinococcosis in tibetan sheep on the qinghai-tibetan plateau, china
The unique geographical and ecological conditions of the Qinghai-Tibetan Plateau (QTP) constitute the environmental conditions of the natural foci of cystic echinococcosis (CE). It is a rare and highly endemic area in the world. The main host of the pathogen, Tibetan sheep, is widely distributed, an...
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| Format: | Article |
| Language: | English |
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Kafkas University, Faculty of Veterinary Medicine
2021-01-01
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| Series: | Kafkas Universitesi Veteriner Fakültesi Dergisi |
| Subjects: | |
| Online Access: | https://vetdergikafkas.org/pdf.php?id=2776 |
| _version_ | 1797800681157951488 |
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| author | Hong DUO Yong FU Xiuying SHEN Zhihong GUO Xueyong ZHANG Yijuan MA Mohammed YOSRI Tiancong SUN Youlu CHEN Gengqiu JIUNAI |
| author_facet | Hong DUO Yong FU Xiuying SHEN Zhihong GUO Xueyong ZHANG Yijuan MA Mohammed YOSRI Tiancong SUN Youlu CHEN Gengqiu JIUNAI |
| author_sort | Hong DUO |
| collection | DOAJ |
| description | The unique geographical and ecological conditions of the Qinghai-Tibetan Plateau (QTP) constitute the environmental conditions of the natural foci
of cystic echinococcosis (CE). It is a rare and highly endemic area in the world. The main host of the pathogen, Tibetan sheep, is widely distributed,
and the population density is on the rise in recent years, which increases the pressure on the transmission and risk of the natural epidemic source.
Currently, the developments of serological diagnosis test for Echinococcus granulosus infection on the QTP Tibetan sheep are scanty, which seriously
restricts the epidemiological investigation of CE in Tibetan sheep on the QTP. EgAgB8/2 is one of the most important targets of host antibody response
in CE and has better diagnostic performance in livestock. This study expressed the recombinant EgAgB8/2 antigen of E. granulosus from Tibetan
sheep in prokaryotic expression vector, and also preliminarily evaluated its potential value for diagnosing CE in Tibetan sheep using indirect ELISA.
Our preliminary results shown that the recombinant EgAgB8/2 antigen had good immunogenicity and exhibited high sensitivity (95%) and high
specificity (100%) and no cross-reacted with both Taenia multiceps and Taenia hydatigena. Further studies are needed to collect more Tibetan sheep
sera from E. granulosus and other parasitic infections, which may evaluate further sensitivity, specificity and cross-reactive of recombinant EgAgB8/2
antigen in Tibetan sheep positive for CE of the QTP, China. |
| first_indexed | 2024-03-13T04:38:57Z |
| format | Article |
| id | doaj.art-0b6a4d1e3ec6407c9f8b4f8a3ad6c288 |
| institution | Directory Open Access Journal |
| issn | 1309-2251 |
| language | English |
| last_indexed | 2024-03-13T04:38:57Z |
| publishDate | 2021-01-01 |
| publisher | Kafkas University, Faculty of Veterinary Medicine |
| record_format | Article |
| series | Kafkas Universitesi Veteriner Fakültesi Dergisi |
| spelling | doaj.art-0b6a4d1e3ec6407c9f8b4f8a3ad6c2882023-06-19T06:30:05ZengKafkas University, Faculty of Veterinary MedicineKafkas Universitesi Veteriner Fakültesi Dergisi1309-22512021-01-0127220320810.9775/kvfd.2020.250672776Evaluation of the recombinant egagb8/2 antigen for the diagnosis of cystic echinococcosis in tibetan sheep on the qinghai-tibetan plateau, chinaHong DUO0Yong FU1Xiuying SHEN2Zhihong GUO3Xueyong ZHANG4Yijuan MAMohammed YOSRI5Tiancong SUN6Youlu CHEN7Gengqiu JIUNAI8Qinghai Academy of Animal Science and Veterinary Medicine, Qinghai University, State Key Laboratory of Plateau Ecology and Agriculture, Xining 810016, CHINAQinghai Academy of Animal Science and Veterinary Medicine, Qinghai University, State Key Laboratory of Plateau Ecology and Agriculture, Xining 810016, CHINAQinghai Academy of Animal Science and Veterinary Medicine, Qinghai University, State Key Laboratory of Plateau Ecology and Agriculture, Xining 810016, CHINAQinghai Academy of Animal Science and Veterinary Medicine, Qinghai University, State Key Laboratory of Plateau Ecology and Agriculture, Xining 810016, CHINAQinghai Academy of Animal Science and Veterinary Medicine, Qinghai University, State Key Laboratory of Plateau Ecology and Agriculture, Xining 810016, CHINAThe Regional Center for Mycology and Biotechnology, Al-Azhar University, Cairo 11787, EGYPTQinghai Academy of Animal Science and Veterinary Medicine, Qinghai University, State Key Laboratory of Plateau Ecology and Agriculture, Xining 810016, CHINAQinghai Academy of Animal Science and Veterinary Medicine, Qinghai University, State Key Laboratory of Plateau Ecology and Agriculture, Xining 810016, CHINAAnimal Husbandry and Veterinary Station, Zhenqin Township, Chindu County 815100, CHINAThe unique geographical and ecological conditions of the Qinghai-Tibetan Plateau (QTP) constitute the environmental conditions of the natural foci of cystic echinococcosis (CE). It is a rare and highly endemic area in the world. The main host of the pathogen, Tibetan sheep, is widely distributed, and the population density is on the rise in recent years, which increases the pressure on the transmission and risk of the natural epidemic source. Currently, the developments of serological diagnosis test for Echinococcus granulosus infection on the QTP Tibetan sheep are scanty, which seriously restricts the epidemiological investigation of CE in Tibetan sheep on the QTP. EgAgB8/2 is one of the most important targets of host antibody response in CE and has better diagnostic performance in livestock. This study expressed the recombinant EgAgB8/2 antigen of E. granulosus from Tibetan sheep in prokaryotic expression vector, and also preliminarily evaluated its potential value for diagnosing CE in Tibetan sheep using indirect ELISA. Our preliminary results shown that the recombinant EgAgB8/2 antigen had good immunogenicity and exhibited high sensitivity (95%) and high specificity (100%) and no cross-reacted with both Taenia multiceps and Taenia hydatigena. Further studies are needed to collect more Tibetan sheep sera from E. granulosus and other parasitic infections, which may evaluate further sensitivity, specificity and cross-reactive of recombinant EgAgB8/2 antigen in Tibetan sheep positive for CE of the QTP, China.https://vetdergikafkas.org/pdf.php?id=2776cystic echinococcosistibetan sheepegagb8/2 antigenserological diagnosis |
| spellingShingle | Hong DUO Yong FU Xiuying SHEN Zhihong GUO Xueyong ZHANG Yijuan MA Mohammed YOSRI Tiancong SUN Youlu CHEN Gengqiu JIUNAI Evaluation of the recombinant egagb8/2 antigen for the diagnosis of cystic echinococcosis in tibetan sheep on the qinghai-tibetan plateau, china Kafkas Universitesi Veteriner Fakültesi Dergisi cystic echinococcosis tibetan sheep egagb8/2 antigen serological diagnosis |
| title | Evaluation of the recombinant egagb8/2 antigen for the diagnosis of cystic echinococcosis in tibetan sheep on the qinghai-tibetan plateau, china |
| title_full | Evaluation of the recombinant egagb8/2 antigen for the diagnosis of cystic echinococcosis in tibetan sheep on the qinghai-tibetan plateau, china |
| title_fullStr | Evaluation of the recombinant egagb8/2 antigen for the diagnosis of cystic echinococcosis in tibetan sheep on the qinghai-tibetan plateau, china |
| title_full_unstemmed | Evaluation of the recombinant egagb8/2 antigen for the diagnosis of cystic echinococcosis in tibetan sheep on the qinghai-tibetan plateau, china |
| title_short | Evaluation of the recombinant egagb8/2 antigen for the diagnosis of cystic echinococcosis in tibetan sheep on the qinghai-tibetan plateau, china |
| title_sort | evaluation of the recombinant egagb8 2 antigen for the diagnosis of cystic echinococcosis in tibetan sheep on the qinghai tibetan plateau china |
| topic | cystic echinococcosis tibetan sheep egagb8/2 antigen serological diagnosis |
| url | https://vetdergikafkas.org/pdf.php?id=2776 |
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