Two Main Tropane Alkaloids Variations of Black Henbane (Hyoscyamus niger) Under PGPRs Inoculation and Water Deficit Stress Induction at Flowering Stage
Background: Plants infection with microorganisms as well as physical factors such as osmotic stresses especially drought stress induced particular secondary metabolite production. Objective: Plant root and shoot hyoscyamine (HYO) and scopolamine (SCO) variations were investigated under three water d...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Institue of Medicinal Plants, ACECR
2013-02-01
|
| Series: | Journal of Medicinal Plants |
| Subjects: | |
| Online Access: | http://jmp.ir/article-1-107-en.html |
| _version_ | 1818664322223570944 |
|---|---|
| author | M Ghorbanpour R Ghafarzadegan K Khavazi M Hatami |
| author_facet | M Ghorbanpour R Ghafarzadegan K Khavazi M Hatami |
| author_sort | M Ghorbanpour |
| collection | DOAJ |
| description | Background: Plants infection with microorganisms as well as physical factors such as osmotic stresses especially drought stress induced particular secondary metabolite production. Objective: Plant root and shoot hyoscyamine (HYO) and scopolamine (SCO) variations were investigated under three water deficit stress (WDS) levels as 30 (W1), 60 (W2) and 90 % (W3) water depletion of field capacity and Pseudomonas putida (PP) and P. fluoresence (PF) strains inoculation at flowering stage. Methods: Seedling radicles and culture media were inoculated with PP and PF (109 CFU/ml) strains. Monitoring of soil moisture levels was conducted using gravimetric method. Alkaloids extracted were identified by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS) analysis. Results: Results revealed that HYO was the prevalent alkaloid in root and shoot organs. The highest ratio of SCO to HYO (0.53) and the highest alkaloids both HYO and SCO content in root and shoot was observed in W3PF treatment. The largest total alkaloids (HYO+SCO) yield (25.7 mg.plant-1) was showed in PP treated plants under W1 conditions. Conclusion: Integrative use of effective Pseudomonades strains and WDS sounds to be an encouraging and eco-friendly strategy for increasing tropane alkaloids yield and content in Hyoscyamus niger root and shoot parts. |
| first_indexed | 2024-12-17T05:30:54Z |
| format | Article |
| id | doaj.art-0b6e820dea564b14b3227ed4ca786732 |
| institution | Directory Open Access Journal |
| issn | 2717-204X 2717-2058 |
| language | English |
| last_indexed | 2024-12-17T05:30:54Z |
| publishDate | 2013-02-01 |
| publisher | Institue of Medicinal Plants, ACECR |
| record_format | Article |
| series | Journal of Medicinal Plants |
| spelling | doaj.art-0b6e820dea564b14b3227ed4ca7867322022-12-21T22:01:43ZengInstitue of Medicinal Plants, ACECRJournal of Medicinal Plants2717-204X2717-20582013-02-0112452942Two Main Tropane Alkaloids Variations of Black Henbane (Hyoscyamus niger) Under PGPRs Inoculation and Water Deficit Stress Induction at Flowering StageM Ghorbanpour0R Ghafarzadegan1K Khavazi2M Hatami3 Department of Medicinal Plants, Faculty of Agriculture and Natural Resources, Arak University Pharmacognosy & Pharmaceutics Department of Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR Department of Soil Biology, Soil and Water Research Institute Department of Horticultural Sciences, Faculty of Agriculture, Guilan University Background: Plants infection with microorganisms as well as physical factors such as osmotic stresses especially drought stress induced particular secondary metabolite production. Objective: Plant root and shoot hyoscyamine (HYO) and scopolamine (SCO) variations were investigated under three water deficit stress (WDS) levels as 30 (W1), 60 (W2) and 90 % (W3) water depletion of field capacity and Pseudomonas putida (PP) and P. fluoresence (PF) strains inoculation at flowering stage. Methods: Seedling radicles and culture media were inoculated with PP and PF (109 CFU/ml) strains. Monitoring of soil moisture levels was conducted using gravimetric method. Alkaloids extracted were identified by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS) analysis. Results: Results revealed that HYO was the prevalent alkaloid in root and shoot organs. The highest ratio of SCO to HYO (0.53) and the highest alkaloids both HYO and SCO content in root and shoot was observed in W3PF treatment. The largest total alkaloids (HYO+SCO) yield (25.7 mg.plant-1) was showed in PP treated plants under W1 conditions. Conclusion: Integrative use of effective Pseudomonades strains and WDS sounds to be an encouraging and eco-friendly strategy for increasing tropane alkaloids yield and content in Hyoscyamus niger root and shoot parts.http://jmp.ir/article-1-107-en.htmlhyoscyamus nigerpgpr (plant growth promoting rhizobacteria)tropane alkaloidswater deficit stress |
| spellingShingle | M Ghorbanpour R Ghafarzadegan K Khavazi M Hatami Two Main Tropane Alkaloids Variations of Black Henbane (Hyoscyamus niger) Under PGPRs Inoculation and Water Deficit Stress Induction at Flowering Stage Journal of Medicinal Plants hyoscyamus niger pgpr (plant growth promoting rhizobacteria) tropane alkaloids water deficit stress |
| title | Two Main Tropane Alkaloids Variations of Black Henbane (Hyoscyamus niger) Under PGPRs Inoculation and Water Deficit Stress Induction at Flowering Stage |
| title_full | Two Main Tropane Alkaloids Variations of Black Henbane (Hyoscyamus niger) Under PGPRs Inoculation and Water Deficit Stress Induction at Flowering Stage |
| title_fullStr | Two Main Tropane Alkaloids Variations of Black Henbane (Hyoscyamus niger) Under PGPRs Inoculation and Water Deficit Stress Induction at Flowering Stage |
| title_full_unstemmed | Two Main Tropane Alkaloids Variations of Black Henbane (Hyoscyamus niger) Under PGPRs Inoculation and Water Deficit Stress Induction at Flowering Stage |
| title_short | Two Main Tropane Alkaloids Variations of Black Henbane (Hyoscyamus niger) Under PGPRs Inoculation and Water Deficit Stress Induction at Flowering Stage |
| title_sort | two main tropane alkaloids variations of black henbane hyoscyamus niger under pgprs inoculation and water deficit stress induction at flowering stage |
| topic | hyoscyamus niger pgpr (plant growth promoting rhizobacteria) tropane alkaloids water deficit stress |
| url | http://jmp.ir/article-1-107-en.html |
| work_keys_str_mv | AT mghorbanpour twomaintropanealkaloidsvariationsofblackhenbanehyoscyamusnigerunderpgprsinoculationandwaterdeficitstressinductionatfloweringstage AT rghafarzadegan twomaintropanealkaloidsvariationsofblackhenbanehyoscyamusnigerunderpgprsinoculationandwaterdeficitstressinductionatfloweringstage AT kkhavazi twomaintropanealkaloidsvariationsofblackhenbanehyoscyamusnigerunderpgprsinoculationandwaterdeficitstressinductionatfloweringstage AT mhatami twomaintropanealkaloidsvariationsofblackhenbanehyoscyamusnigerunderpgprsinoculationandwaterdeficitstressinductionatfloweringstage |