Salinity inhibits seed germination and embryo growth by reducing starch mobilization efficiency in barley
Abstract Barley is one of the world's earliest domesticated crops, which is widely used for beer production, animal feeding, and health care. Barley seed germination, particularly in increasingly saline soils, is key to ensure the safety of crop production. However, the mechanism of salt‐affect...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2024-02-01
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| Series: | Plant Direct |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/pld3.564 |
| _version_ | 1797294313186525184 |
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| author | Min Xiong Jian Xu Zhou Zhou Bin Peng Yuxiang Shen Huiquan Shen Xiao Xu Changya Li Lina Deng Gongneng Feng |
| author_facet | Min Xiong Jian Xu Zhou Zhou Bin Peng Yuxiang Shen Huiquan Shen Xiao Xu Changya Li Lina Deng Gongneng Feng |
| author_sort | Min Xiong |
| collection | DOAJ |
| description | Abstract Barley is one of the world's earliest domesticated crops, which is widely used for beer production, animal feeding, and health care. Barley seed germination, particularly in increasingly saline soils, is key to ensure the safety of crop production. However, the mechanism of salt‐affected seed germination in barley remains elusive. Here, two different colored barley varieties were used to independently study the regulation mechanism of salt tolerance during barley seed germination. High salinity delays barley seed germination by slowing down starch mobilization efficiency in seeds. The starch plate test revealed that salinity had a significant inhibitory effect on α‐amylase activity in barley seeds. Further, NaCl treatment down‐regulated the expression of Amy1, Amy2 and Amy3 genes in germinated seeds, thereby inhibiting α‐amylase activity. In addition, the result of embryogenic culture system in vitro showed that the shoot elongation of barley was significantly inhibited by salt stress. These findings indicate that it is a feasible idea to study the regulation mechanism of salinity on barley seed germination and embryo growth from the aspect of starch‐related source‐sink communication. |
| first_indexed | 2024-03-07T21:29:23Z |
| format | Article |
| id | doaj.art-0b73d566d00c469088981113f77014b2 |
| institution | Directory Open Access Journal |
| issn | 2475-4455 |
| language | English |
| last_indexed | 2024-03-07T21:29:23Z |
| publishDate | 2024-02-01 |
| publisher | Wiley |
| record_format | Article |
| series | Plant Direct |
| spelling | doaj.art-0b73d566d00c469088981113f77014b22024-02-27T03:05:01ZengWileyPlant Direct2475-44552024-02-0182n/an/a10.1002/pld3.564Salinity inhibits seed germination and embryo growth by reducing starch mobilization efficiency in barleyMin Xiong0Jian Xu1Zhou Zhou2Bin Peng3Yuxiang Shen4Huiquan Shen5Xiao Xu6Changya Li7Lina Deng8Gongneng Feng9College of Marine and Biology Engineering Yancheng Institute of Technology Yancheng Jiangsu ChinaCollege of Marine and Biology Engineering Yancheng Institute of Technology Yancheng Jiangsu ChinaCollege of Marine and Biology Engineering Yancheng Institute of Technology Yancheng Jiangsu ChinaCollege of Marine and Biology Engineering Yancheng Institute of Technology Yancheng Jiangsu ChinaCollege of Marine and Biology Engineering Yancheng Institute of Technology Yancheng Jiangsu ChinaJiangsu Coastal Area Institute of Agricultural Sciences Yancheng Jiangsu ChinaJiangsu Coastal Area Institute of Agricultural Sciences Yancheng Jiangsu ChinaYancheng Grain and Oil Crop Technical Guidance Station Yancheng Jiangsu ChinaCollege of Marine and Biology Engineering Yancheng Institute of Technology Yancheng Jiangsu ChinaCollege of Marine and Biology Engineering Yancheng Institute of Technology Yancheng Jiangsu ChinaAbstract Barley is one of the world's earliest domesticated crops, which is widely used for beer production, animal feeding, and health care. Barley seed germination, particularly in increasingly saline soils, is key to ensure the safety of crop production. However, the mechanism of salt‐affected seed germination in barley remains elusive. Here, two different colored barley varieties were used to independently study the regulation mechanism of salt tolerance during barley seed germination. High salinity delays barley seed germination by slowing down starch mobilization efficiency in seeds. The starch plate test revealed that salinity had a significant inhibitory effect on α‐amylase activity in barley seeds. Further, NaCl treatment down‐regulated the expression of Amy1, Amy2 and Amy3 genes in germinated seeds, thereby inhibiting α‐amylase activity. In addition, the result of embryogenic culture system in vitro showed that the shoot elongation of barley was significantly inhibited by salt stress. These findings indicate that it is a feasible idea to study the regulation mechanism of salinity on barley seed germination and embryo growth from the aspect of starch‐related source‐sink communication.https://doi.org/10.1002/pld3.564barleysalinityseed germinationstarch mobilizationα‐Amylase activity |
| spellingShingle | Min Xiong Jian Xu Zhou Zhou Bin Peng Yuxiang Shen Huiquan Shen Xiao Xu Changya Li Lina Deng Gongneng Feng Salinity inhibits seed germination and embryo growth by reducing starch mobilization efficiency in barley Plant Direct barley salinity seed germination starch mobilization α‐Amylase activity |
| title | Salinity inhibits seed germination and embryo growth by reducing starch mobilization efficiency in barley |
| title_full | Salinity inhibits seed germination and embryo growth by reducing starch mobilization efficiency in barley |
| title_fullStr | Salinity inhibits seed germination and embryo growth by reducing starch mobilization efficiency in barley |
| title_full_unstemmed | Salinity inhibits seed germination and embryo growth by reducing starch mobilization efficiency in barley |
| title_short | Salinity inhibits seed germination and embryo growth by reducing starch mobilization efficiency in barley |
| title_sort | salinity inhibits seed germination and embryo growth by reducing starch mobilization efficiency in barley |
| topic | barley salinity seed germination starch mobilization α‐Amylase activity |
| url | https://doi.org/10.1002/pld3.564 |
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