Comparative analysis of QS3D versus droplet digital PCR for quantitative measures of EGFR T790M mutation from identical plasma

Comparative analysis of QS3D versus droplet digital PCR for quantitative measures of EGFR T790M mutation from identical plasma

Objectives: The capacity of QuantStudio™ 3D (QS3D) and droplet digital PCR (dPCR) for the detection of plasma Epidermal Growth Factor Receptor (EGFR) mutations have been widely reported. Few comparative studies on the quantitative test of the identical DNA material, however, are carried out. Here we...

Full description

Bibliographic Details
Main Authors:	Qiaomei Guo, Lin Wang, Xiaohui Liang, Mingna Zhao, Xia Huang, Wanxing Xu, Jiatao Lou, Lihua Qiao
Format:	Article
Language:	English
Published:	Elsevier 2022-11-01
Series:	Heliyon
Subjects:	cfDNA Concordance Digital PCR Mutation abundance T790M
Online Access:	http://www.sciencedirect.com/science/article/pii/S2405844022026275

Similar Items

The Challenge to Stabilize, Extract and Analyze Urinary Cell-Free DNA (ucfDNA) during Clinical Routine
by: Ivonne Nel, et al.
Published: (2023-12-01)

Droplet Digital PCR as a Molecular Tool for the Detection of the EGFR T790M Mutation in NSCLC Patients with the EGFR Activating Mutations
by: Durgut S, et al.
Published: (2024-03-01)

Graft-Derived Cell-Free DNA Quantification following Liver Transplantation Using Tissue-Specific DNA Methylation and Donor-Specific Genotyping Techniques: An Orthogonal Comparison Study
by: Daniel R. A. Cox, et al.
Published: (2023-06-01)

Fragment size and level of cell-free DNA provide prognostic information in patients with advanced pancreatic cancer
by: Morten Lapin, et al.
Published: (2018-11-01)

Factors affecting couples’ decision making about expanded prenatal cell-free DNA screening
by: Kelsey Mumford, et al.
Published: (2024-01-01)

BL-MOL-AR Project, Preliminary Results about Liquid Biopsy: Molecular Approach Experience and Research Activity in Oncological Settings
by: Pancrazzi Pancrazzi, et al.
Published: (2023-09-01)

The cfDNA in early cancer detection
by: Hussein Adil Abid, et al.
Published: (2024-01-01)

Circulating cell-free DNA as a biomarker for diagnosis of Schistosomiasis japonica
by: Yu Zhang, et al.
Published: (2024-03-01)

Targeted fetal cell‐free DNA screening for aneuploidies in 4,594 pregnancies: Single center study
by: Altug Koc, et al.
Published: (2019-07-01)

Technical Evaluation of the COBAS EGFR Semiquantitative Index (SQI) for Plasma cfDNA Testing in NSCLC Patients with EGFR Exon 19 Deletions
by: José Manuel González de Aledo-Castillo, et al.
Published: (2021-07-01)

Cell‐free DNA‐associated multi‐feature applications in cancer diagnosis and treatment
by: Xiaolu Zhang, et al.
Published: (2024-04-01)

Biguanide chitosan microneedles with cell-free DNA scavenging ability for psoriasis therapy
by: Zihao Liu, et al.
Published: (2024-03-01)

Neutrophil extracellular traps in stored canine red blood cell units
by: Erin R. McQuinn, et al.
Published: (2020-09-01)

Comparing cell‐free circulating tumor DNA mutational profiles of disease‐free and nonresponders patients with oropharyngeal squamous cell carcinoma
by: Alok R. Khandelwal, et al.
Published: (2020-10-01)

Evaluation of commercial kits for isolation and bisulfite conversion of circulating cell-free tumor DNA from blood
by: Stine H. Kresse, et al.
Published: (2023-09-01)

Application of cell-free DNA sequencing in characterization of bloodborne microbes and the study of microbe-disease interactions
by: Kuo-Ping Chiu, et al.
Published: (2019-08-01)

Detecting cancer from cell-free DNA sequencing fragmentation patterns
by: Chen, L
Published: (2023)

A review of cell-free DNA and epigenetics for non-invasive diagnosis in solid organ transplantation
by: Alizée Sebastian, et al.
Published: (2024-11-01)

Role of cell-free DNA and extracellular vesicles for diagnosis and surveillance in patients with glioma
by: Busra Karacam, et al.
Published: (2024-06-01)

Early detection of uterine corpus endometrial carcinoma utilizing plasma cfDNA fragmentomics
by: Jing Liu, et al.
Published: (2024-07-01)

Discovery and Validation of Methylation Signatures in Circulating Cell-Free DNA for the Detection of Colorectal Cancer
by: Zhiping Long, et al.
Published: (2024-08-01)

Establishment and validation of circulating cell-free DNA signatures for nasopharyngeal carcinoma detectionResearch in context
by: Su-Fang Qiu, et al.
Published: (2024-10-01)

A Fully Integrated Microfluidic Device with Immobilized Dyes for Simultaneous Detection of Cell-Free DNA and Histones from Plasma Using Dehydrated Agarose Gates
by: Shadi Shahriari, et al.
Published: (2024-03-01)

Effect of acute moderate-intensity cycling on cfDNA levels considering menstrual cycle phases
by: Akemi Sawai, et al.
Published: (2024-01-01)

IDH1 mutation is detectable in plasma cell-free DNA and is associated with survival outcome in glioma patients
by: Stefania Crucitta, et al.
Published: (2024-01-01)

Rapid and Efficient Extraction of Cell-Free DNA Using Homobifunctional Crosslinkers
by: HyeonAh Seong, et al.
Published: (2022-08-01)

Unlocking the secrets: the power of methylation-based cfDNA detection of tissue damage in organ systems
by: Lijing Zhang, et al.
Published: (2023-10-01)

Cell-Free DNA in Plasma and Serum Indicates Disease Severity and Prognosis in Blunt Trauma Patients
by: Inga Trulson, et al.
Published: (2023-03-01)

Neutrophil-dependent Mitochondrial DNA Release Associated With Extracellular Trap Formation in Inflammatory Bowel Disease
by: Broc Drury, et al.
Published: (2023-01-01)

Quantification of human papillomavirus cell-free DNA from low-volume blood plasma samples by digital PCR
by: Fabian Rosing, et al.
Published: (2024-07-01)

The Impact of On-Target Resistance Mediated by EGFR-T790M or EGFR-C797S on EGFR Exon 20 Insertion Mutation Active Tyrosine Kinase Inhibitors
by: Ikei S. Kobayashi, MD, PhD, et al.
Published: (2024-01-01)

Clinical utility of repeated rebiopsy for EGFR T790M mutation detection in non-small cell lung cancer
by: Eun Hye Lee, et al.
Published: (2024-08-01)

Application of ddPCR in detection of the status and abundance of EGFR T790M mutation in the plasma samples of non-small cell lung cancer patients
by: Hui Zhang, et al.
Published: (2023-01-01)

Diagnostic Accuracy of Droplet Digital PCR and Amplification Refractory Mutation System PCR for Detecting EGFR Mutation in Cell-Free DNA of Lung Cancer: A Meta-Analysis
by: Caichen Li, et al.
Published: (2020-03-01)

Identification of Candidate Biomarkers for EGFR-T790M Drug-resistant  Gene Mutation in Advanced Lung Adenocarcinoma
by: Lijuan CHEN, et al.
Published: (2020-11-01)

Rebiopsy with Thoracoscopy under Local Anesthesia for the Detection of EGFR T790M Mutation
by: Asako Matsuda, et al.
Published: (2019-12-01)

Combined plasma and tissue genotyping of EGFR T790M benefits NSCLC patients: a real‐world clinical example
by: Bing Wei, et al.
Published: (2019-05-01)

Unveiling the impact of corticosteroid therapy on liquid biopsy-detected cell-free DNA levels in meningioma and glioblastoma patients
by: Veronica Aran, et al.
Published: (2024-09-01)

Donor-derived cell-free DNA in chronic lung allograft dysfunction phenotypes: a pilot study
by: H. Beeckmans, et al.
Published: (2024-12-01)

Combination of Hotspot Mutations With Methylation and Fragmentomic Profiles to Enhance Multi‐Cancer Early Detection
by: Thi Hue Hanh Nguyen, et al.
Published: (2025-01-01)