Biological activity of peptides isolated from feather keratin waste through microbial and enzymatic hydrolysis
Background: A major portion of poultry feather waste is constituted by keratin, which is recalcitrant to degradation by common proteases. Feather waste contributes to a significant volume of biowaste load to the environment. Valorization of these wastes into various products has been attempted by ma...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-11-01
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| Series: | Electronic Journal of Biotechnology |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S071734582200032X |
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| author | Pintubala Kshetri Pangambam Langamba Singh Shamjetshabam Babeeta Chanu Thangjam Surchandra Singh Chongtham Rajiv K Tamreihao Heikham Naresh Singh Tania Chongtham Asem Kajal Devi Susheel Kumar Sharma Sonia Chongtham M. Norjit Singh Y. Prabhabati Devi Haobijam Sanjita Devi Subhra Saikat Roy |
| author_facet | Pintubala Kshetri Pangambam Langamba Singh Shamjetshabam Babeeta Chanu Thangjam Surchandra Singh Chongtham Rajiv K Tamreihao Heikham Naresh Singh Tania Chongtham Asem Kajal Devi Susheel Kumar Sharma Sonia Chongtham M. Norjit Singh Y. Prabhabati Devi Haobijam Sanjita Devi Subhra Saikat Roy |
| author_sort | Pintubala Kshetri |
| collection | DOAJ |
| description | Background: A major portion of poultry feather waste is constituted by keratin, which is recalcitrant to degradation by common proteases. Feather waste contributes to a significant volume of biowaste load to the environment. Valorization of these wastes into various products has been attempted by many researchers. The present study aimed to produce peptides (molecular weight < 10 kDa) from feather waste by the action of keratinolytic bacteria or keratinase enzyme and to screen the peptides for pharmaceutical and therapeutic properties. The feathers were subjected to hydrolysis by using locally isolated keratinolytic microorganisms, namely Streptomyces tanashiensis-RCM-SSR-6, Bacillus sp. RCM-SSR-102, and purified keratinase enzyme KER-102. Results: The feather keratin hydrolysate obtained by hydrolysis with different bacterial species/enzymes showed different protein profiles in SDS-PAGE. As indicated by Fourier Transform Infrared Spectroscopy (FTIR) analysis, a difference was observed in the composition of α-helix and β-sheet in the peptides produced by different microbial/enzymatic methods. The peptides were screened for antioxidant potential, antityrosinase property, and inhibitory activity against angiotensin-converting enzyme (ACE), lipoxygenase, and xanthine oxidase. The peptides showed promising results in all the assays, except peptide-102 that did not show ACE inhibitory activity. Interestingly, the crude peptide-6 (4.06 µg/mL) and peptide-102 (10.21 µg/mL) showed a lower EC50 value than the standard Kojic acid (27.04 µg/mL) in antityrosinase assay. Conclusions: Degradation of chicken feather waste with microbial or enzymatic method is an eco-friendly approach to yield diverse bioactive peptides. Hence, the present study established that feather keratin could be a potential source of many health-beneficial peptides.How to cite: Kshetri P, Singh PL, Chanu SB, et al. Biological activity of peptides isolated from feather keratin waste through microbial and enzymatic hydrolysis. Electron J Biotechnol 2022;60. https://doi.org/10.1016/j.ejbt.2022.08.001. |
| first_indexed | 2024-04-11T11:31:00Z |
| format | Article |
| id | doaj.art-0b78e06db52a4f96ae3386172fd4f148 |
| institution | Directory Open Access Journal |
| issn | 0717-3458 |
| language | English |
| last_indexed | 2024-04-11T11:31:00Z |
| publishDate | 2022-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Electronic Journal of Biotechnology |
| spelling | doaj.art-0b78e06db52a4f96ae3386172fd4f1482022-12-22T04:26:07ZengElsevierElectronic Journal of Biotechnology0717-34582022-11-01601118Biological activity of peptides isolated from feather keratin waste through microbial and enzymatic hydrolysisPintubala Kshetri0Pangambam Langamba Singh1Shamjetshabam Babeeta Chanu2Thangjam Surchandra Singh3Chongtham Rajiv4K Tamreihao5Heikham Naresh Singh6Tania Chongtham7Asem Kajal Devi8Susheel Kumar Sharma9Sonia Chongtham10M. Norjit Singh11Y. Prabhabati Devi12Haobijam Sanjita Devi13Subhra Saikat Roy14ICAR-NEH Quality Analysis Laboratory, ICAR Research Complex for NEH Region, Manipur Centre, Imphal, IndiaICAR-NEH Quality Analysis Laboratory, ICAR Research Complex for NEH Region, Manipur Centre, Imphal, IndiaManipur University, Imphal, IndiaICAR-NEH Quality Analysis Laboratory, ICAR Research Complex for NEH Region, Manipur Centre, Imphal, IndiaICAR-NEH Quality Analysis Laboratory, ICAR Research Complex for NEH Region, Manipur Centre, Imphal, IndiaICAR-NEH Quality Analysis Laboratory, ICAR Research Complex for NEH Region, Manipur Centre, Imphal, IndiaICAR-NEH Quality Analysis Laboratory, ICAR Research Complex for NEH Region, Manipur Centre, Imphal, IndiaICAR-NEH Quality Analysis Laboratory, ICAR Research Complex for NEH Region, Manipur Centre, Imphal, IndiaICAR-NEH Quality Analysis Laboratory, ICAR Research Complex for NEH Region, Manipur Centre, Imphal, IndiaICAR-NEH Quality Analysis Laboratory, ICAR Research Complex for NEH Region, Manipur Centre, Imphal, IndiaICAR-NEH Quality Analysis Laboratory, ICAR Research Complex for NEH Region, Manipur Centre, Imphal, IndiaCollege of Agriculture, Central Agricultural University, Imphal, IndiaICAR-NEH Quality Analysis Laboratory, ICAR Research Complex for NEH Region, Manipur Centre, Imphal, IndiaCollege of Horticulture, Central Agricultural University, Sikkim, IndiaICAR-NEH Quality Analysis Laboratory, ICAR Research Complex for NEH Region, Manipur Centre, Imphal, India; Corresponding author.Background: A major portion of poultry feather waste is constituted by keratin, which is recalcitrant to degradation by common proteases. Feather waste contributes to a significant volume of biowaste load to the environment. Valorization of these wastes into various products has been attempted by many researchers. The present study aimed to produce peptides (molecular weight < 10 kDa) from feather waste by the action of keratinolytic bacteria or keratinase enzyme and to screen the peptides for pharmaceutical and therapeutic properties. The feathers were subjected to hydrolysis by using locally isolated keratinolytic microorganisms, namely Streptomyces tanashiensis-RCM-SSR-6, Bacillus sp. RCM-SSR-102, and purified keratinase enzyme KER-102. Results: The feather keratin hydrolysate obtained by hydrolysis with different bacterial species/enzymes showed different protein profiles in SDS-PAGE. As indicated by Fourier Transform Infrared Spectroscopy (FTIR) analysis, a difference was observed in the composition of α-helix and β-sheet in the peptides produced by different microbial/enzymatic methods. The peptides were screened for antioxidant potential, antityrosinase property, and inhibitory activity against angiotensin-converting enzyme (ACE), lipoxygenase, and xanthine oxidase. The peptides showed promising results in all the assays, except peptide-102 that did not show ACE inhibitory activity. Interestingly, the crude peptide-6 (4.06 µg/mL) and peptide-102 (10.21 µg/mL) showed a lower EC50 value than the standard Kojic acid (27.04 µg/mL) in antityrosinase assay. Conclusions: Degradation of chicken feather waste with microbial or enzymatic method is an eco-friendly approach to yield diverse bioactive peptides. Hence, the present study established that feather keratin could be a potential source of many health-beneficial peptides.How to cite: Kshetri P, Singh PL, Chanu SB, et al. Biological activity of peptides isolated from feather keratin waste through microbial and enzymatic hydrolysis. Electron J Biotechnol 2022;60. https://doi.org/10.1016/j.ejbt.2022.08.001.http://www.sciencedirect.com/science/article/pii/S071734582200032XBioactive peptidesBiowasteEco-friendlyFeatherHydrolysisKeratin |
| spellingShingle | Pintubala Kshetri Pangambam Langamba Singh Shamjetshabam Babeeta Chanu Thangjam Surchandra Singh Chongtham Rajiv K Tamreihao Heikham Naresh Singh Tania Chongtham Asem Kajal Devi Susheel Kumar Sharma Sonia Chongtham M. Norjit Singh Y. Prabhabati Devi Haobijam Sanjita Devi Subhra Saikat Roy Biological activity of peptides isolated from feather keratin waste through microbial and enzymatic hydrolysis Electronic Journal of Biotechnology Bioactive peptides Biowaste Eco-friendly Feather Hydrolysis Keratin |
| title | Biological activity of peptides isolated from feather keratin waste through microbial and enzymatic hydrolysis |
| title_full | Biological activity of peptides isolated from feather keratin waste through microbial and enzymatic hydrolysis |
| title_fullStr | Biological activity of peptides isolated from feather keratin waste through microbial and enzymatic hydrolysis |
| title_full_unstemmed | Biological activity of peptides isolated from feather keratin waste through microbial and enzymatic hydrolysis |
| title_short | Biological activity of peptides isolated from feather keratin waste through microbial and enzymatic hydrolysis |
| title_sort | biological activity of peptides isolated from feather keratin waste through microbial and enzymatic hydrolysis |
| topic | Bioactive peptides Biowaste Eco-friendly Feather Hydrolysis Keratin |
| url | http://www.sciencedirect.com/science/article/pii/S071734582200032X |
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