Supercritical Impregnation of Mango Leaf Extract into PLA 3D-Printed Devices and Evaluation of Their Biocompatibility with Endothelial Cell Cultures
The addition of natural substances with pharmacoactive properties to polymeric biomedical devices would provide beneficial regarding the assimilation of these endoprostheses when implanted into a patient’s body. The added drug would facilitate endothelization by regulating the inflammatory processes...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-07-01
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| Series: | Polymers |
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| Online Access: | https://www.mdpi.com/2073-4360/14/13/2706 |
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| author | Pilar Grosso Cristina Cejudo Ismael Sánchez-Gomar Mª Carmen Durán-Ruiz Rafael Moreno-Luna Lourdes Casas Clara Pereyra Casimiro Mantell |
| author_facet | Pilar Grosso Cristina Cejudo Ismael Sánchez-Gomar Mª Carmen Durán-Ruiz Rafael Moreno-Luna Lourdes Casas Clara Pereyra Casimiro Mantell |
| author_sort | Pilar Grosso |
| collection | DOAJ |
| description | The addition of natural substances with pharmacoactive properties to polymeric biomedical devices would provide beneficial regarding the assimilation of these endoprostheses when implanted into a patient’s body. The added drug would facilitate endothelization by regulating the inflammatory processes that such interventions entail, preventing contamination hazards and favoring the angiogenesis or formation of blood vessels in the tissue. The present work used mango leaf extract (MLE) obtained through pressurized ethanol for this purpose. Polylactic acid (PLA) in the form of filaments or 3D-printed disks was impregnated by means of supercritical technology with MLE for the culture essays. The release kinetics has been studied and the polymer matrices have been examined by scanning electron microscopy (SEM). The impregnated devices were subjected to in vitro culture of colony-forming endothelial cells. The influence of the different impregnation conditions used for the production of the MLE impregnated polymeric devices on the development of the cell culture was determined by fluorescence microscopy. The best results were obtained from the calcein cultures on 35 °C MLE impregnated into 3D-printed polymer disks. |
| first_indexed | 2024-03-09T03:56:11Z |
| format | Article |
| id | doaj.art-0b7cfd6c66694b448db0e88967581ec3 |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-09T03:56:11Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Polymers |
| spelling | doaj.art-0b7cfd6c66694b448db0e88967581ec32023-12-03T14:19:09ZengMDPI AGPolymers2073-43602022-07-011413270610.3390/polym14132706Supercritical Impregnation of Mango Leaf Extract into PLA 3D-Printed Devices and Evaluation of Their Biocompatibility with Endothelial Cell CulturesPilar Grosso0Cristina Cejudo1Ismael Sánchez-Gomar2Mª Carmen Durán-Ruiz3Rafael Moreno-Luna4Lourdes Casas5Clara Pereyra6Casimiro Mantell7Chemical Engineering and Food Technology Department, Wine and Agrifood Research Institute (IVAGRO), University of Cadiz, Puerto Real, 11519 Cadiz, SpainChemical Engineering and Food Technology Department, Wine and Agrifood Research Institute (IVAGRO), University of Cadiz, Puerto Real, 11519 Cadiz, SpainBiomedicine, Biotechnology and Public Health Department, University of Cadiz, 11002 Cadiz, SpainBiomedicine, Biotechnology and Public Health Department, University of Cadiz, 11002 Cadiz, SpainLaboratory of Neuroinflammation, National Paraplegics Hospital, SESCAM, 45071 Toledo, SpainChemical Engineering and Food Technology Department, Wine and Agrifood Research Institute (IVAGRO), University of Cadiz, Puerto Real, 11519 Cadiz, SpainChemical Engineering and Food Technology Department, Wine and Agrifood Research Institute (IVAGRO), University of Cadiz, Puerto Real, 11519 Cadiz, SpainChemical Engineering and Food Technology Department, Wine and Agrifood Research Institute (IVAGRO), University of Cadiz, Puerto Real, 11519 Cadiz, SpainThe addition of natural substances with pharmacoactive properties to polymeric biomedical devices would provide beneficial regarding the assimilation of these endoprostheses when implanted into a patient’s body. The added drug would facilitate endothelization by regulating the inflammatory processes that such interventions entail, preventing contamination hazards and favoring the angiogenesis or formation of blood vessels in the tissue. The present work used mango leaf extract (MLE) obtained through pressurized ethanol for this purpose. Polylactic acid (PLA) in the form of filaments or 3D-printed disks was impregnated by means of supercritical technology with MLE for the culture essays. The release kinetics has been studied and the polymer matrices have been examined by scanning electron microscopy (SEM). The impregnated devices were subjected to in vitro culture of colony-forming endothelial cells. The influence of the different impregnation conditions used for the production of the MLE impregnated polymeric devices on the development of the cell culture was determined by fluorescence microscopy. The best results were obtained from the calcein cultures on 35 °C MLE impregnated into 3D-printed polymer disks.https://www.mdpi.com/2073-4360/14/13/2706supercritical impregnationmango leaf3D printerPLAECFCs |
| spellingShingle | Pilar Grosso Cristina Cejudo Ismael Sánchez-Gomar Mª Carmen Durán-Ruiz Rafael Moreno-Luna Lourdes Casas Clara Pereyra Casimiro Mantell Supercritical Impregnation of Mango Leaf Extract into PLA 3D-Printed Devices and Evaluation of Their Biocompatibility with Endothelial Cell Cultures Polymers supercritical impregnation mango leaf 3D printer PLA ECFCs |
| title | Supercritical Impregnation of Mango Leaf Extract into PLA 3D-Printed Devices and Evaluation of Their Biocompatibility with Endothelial Cell Cultures |
| title_full | Supercritical Impregnation of Mango Leaf Extract into PLA 3D-Printed Devices and Evaluation of Their Biocompatibility with Endothelial Cell Cultures |
| title_fullStr | Supercritical Impregnation of Mango Leaf Extract into PLA 3D-Printed Devices and Evaluation of Their Biocompatibility with Endothelial Cell Cultures |
| title_full_unstemmed | Supercritical Impregnation of Mango Leaf Extract into PLA 3D-Printed Devices and Evaluation of Their Biocompatibility with Endothelial Cell Cultures |
| title_short | Supercritical Impregnation of Mango Leaf Extract into PLA 3D-Printed Devices and Evaluation of Their Biocompatibility with Endothelial Cell Cultures |
| title_sort | supercritical impregnation of mango leaf extract into pla 3d printed devices and evaluation of their biocompatibility with endothelial cell cultures |
| topic | supercritical impregnation mango leaf 3D printer PLA ECFCs |
| url | https://www.mdpi.com/2073-4360/14/13/2706 |
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