Microalgae Biomass and Bioactive Compounds Change According to the Medium's N and pH
Abstract Microalgae have been widely studied as raw materials for biofuels, food supplements and several high value products. The hypothesis is that the pH and N levels in the culture medium are important factors to increase microalgae biomass production and its by-products. Thus, this study aimed t...
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| Format: | Article |
| Language: | English |
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Instituto de Tecnologia do Paraná (Tecpar)
2023-10-01
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| Series: | Brazilian Archives of Biology and Technology |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132023000100229&tlng=en |
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| author | Helder Rodrigues da Silva Cassio Egidio Cavenaghi Prete Letícia Alana Bertoldo Ernani Abicht Basso Ulisses Zonta de Melo Jordana Mayra Nassar Diva Souza Andrade |
| author_facet | Helder Rodrigues da Silva Cassio Egidio Cavenaghi Prete Letícia Alana Bertoldo Ernani Abicht Basso Ulisses Zonta de Melo Jordana Mayra Nassar Diva Souza Andrade |
| author_sort | Helder Rodrigues da Silva |
| collection | DOAJ |
| description | Abstract Microalgae have been widely studied as raw materials for biofuels, food supplements and several high value products. The hypothesis is that the pH and N levels in the culture medium are important factors to increase microalgae biomass production and its by-products. Thus, this study aimed to evaluate response of Neochloris oleoabundans to sodium nitrate concentration and pH levels by assessing biomass production, pigments, total lipid content and lipid profile of cells. The experimental design was a complete factorial with two factors: sodium nitrate (NaNO3) using the doses of 0.25, 1.12 and 2.5 g L-1 and pH 5.0, 7.0 and 9.0, resulting in nine treatments and three replicates. Differences in the N concentration and pH of the in Bold’s basal medium increased up to 21.1% the production of dry biomass but also up to 36% of lipids and up to of 0.81% of carotenoid concentration in the N. oleoabundans cell. The nuclear magnetic resonance (NMR) analysis of bio-oil showed changes in the lipid profile. Oil extracted from N. oleoabundans cells growing in medium at pH 9 and in 2.5 g L−1 of NaNO3 showed fatty acids with molecular weights similar to crude soybean oil, while the oil from treatment 3 (2.5 NaNO3 g L−1) showed a slight increase in molecular weight. Overall, the best adjustment of the liquid medium to grow N. oleoabundans is at pH 9.0 and with addition of 2.5 g of sodium nitrate. |
| first_indexed | 2024-03-11T16:29:49Z |
| format | Article |
| id | doaj.art-0b7d745eb15a4d47923b6e2dc64c6bd4 |
| institution | Directory Open Access Journal |
| issn | 1678-4324 |
| language | English |
| last_indexed | 2024-03-11T16:29:49Z |
| publishDate | 2023-10-01 |
| publisher | Instituto de Tecnologia do Paraná (Tecpar) |
| record_format | Article |
| series | Brazilian Archives of Biology and Technology |
| spelling | doaj.art-0b7d745eb15a4d47923b6e2dc64c6bd42023-10-24T07:38:28ZengInstituto de Tecnologia do Paraná (Tecpar)Brazilian Archives of Biology and Technology1678-43242023-10-016610.1590/1678-4324-2023210415Microalgae Biomass and Bioactive Compounds Change According to the Medium's N and pHHelder Rodrigues da Silvahttps://orcid.org/0000-0003-0679-4692Cassio Egidio Cavenaghi Pretehttps://orcid.org/0000-0003-0337-6874Letícia Alana Bertoldohttps://orcid.org/0000-0003-0710-8113Ernani Abicht Bassohttps://orcid.org/0000-0001-7554-5206Ulisses Zonta de Melohttps://orcid.org/0000-0002-2776-4268Jordana Mayra Nassarhttps://orcid.org/0000-0002-3175-4900Diva Souza Andradehttps://orcid.org/0000-0003-0761-004XAbstract Microalgae have been widely studied as raw materials for biofuels, food supplements and several high value products. The hypothesis is that the pH and N levels in the culture medium are important factors to increase microalgae biomass production and its by-products. Thus, this study aimed to evaluate response of Neochloris oleoabundans to sodium nitrate concentration and pH levels by assessing biomass production, pigments, total lipid content and lipid profile of cells. The experimental design was a complete factorial with two factors: sodium nitrate (NaNO3) using the doses of 0.25, 1.12 and 2.5 g L-1 and pH 5.0, 7.0 and 9.0, resulting in nine treatments and three replicates. Differences in the N concentration and pH of the in Bold’s basal medium increased up to 21.1% the production of dry biomass but also up to 36% of lipids and up to of 0.81% of carotenoid concentration in the N. oleoabundans cell. The nuclear magnetic resonance (NMR) analysis of bio-oil showed changes in the lipid profile. Oil extracted from N. oleoabundans cells growing in medium at pH 9 and in 2.5 g L−1 of NaNO3 showed fatty acids with molecular weights similar to crude soybean oil, while the oil from treatment 3 (2.5 NaNO3 g L−1) showed a slight increase in molecular weight. Overall, the best adjustment of the liquid medium to grow N. oleoabundans is at pH 9.0 and with addition of 2.5 g of sodium nitrate.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132023000100229&tlng=encarotenoidschlorophylllipid profileNeochloris oleoabundanssodium nitrate |
| spellingShingle | Helder Rodrigues da Silva Cassio Egidio Cavenaghi Prete Letícia Alana Bertoldo Ernani Abicht Basso Ulisses Zonta de Melo Jordana Mayra Nassar Diva Souza Andrade Microalgae Biomass and Bioactive Compounds Change According to the Medium's N and pH Brazilian Archives of Biology and Technology carotenoids chlorophyll lipid profile Neochloris oleoabundans sodium nitrate |
| title | Microalgae Biomass and Bioactive Compounds Change According to the Medium's N and pH |
| title_full | Microalgae Biomass and Bioactive Compounds Change According to the Medium's N and pH |
| title_fullStr | Microalgae Biomass and Bioactive Compounds Change According to the Medium's N and pH |
| title_full_unstemmed | Microalgae Biomass and Bioactive Compounds Change According to the Medium's N and pH |
| title_short | Microalgae Biomass and Bioactive Compounds Change According to the Medium's N and pH |
| title_sort | microalgae biomass and bioactive compounds change according to the medium s n and ph |
| topic | carotenoids chlorophyll lipid profile Neochloris oleoabundans sodium nitrate |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132023000100229&tlng=en |
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