Drosophila simulans: A Species with Improved Resolution in Evolve and Resequence Studies
The combination of experimental evolution with high-throughput sequencing of pooled individuals—i.e., evolve and resequence (E&R)—is a powerful approach to study adaptation from standing genetic variation under controlled, replicated conditions. Nevertheless, E&R studies in Drosophila melano...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Oxford University Press
2017-07-01
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| Series: | G3: Genes, Genomes, Genetics |
| Subjects: | |
| Online Access: | http://g3journal.org/lookup/doi/10.1534/g3.117.043349 |
| _version_ | 1819149172257849344 |
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| author | Neda Barghi Raymond Tobler Viola Nolte Christian Schlötterer |
| author_facet | Neda Barghi Raymond Tobler Viola Nolte Christian Schlötterer |
| author_sort | Neda Barghi |
| collection | DOAJ |
| description | The combination of experimental evolution with high-throughput sequencing of pooled individuals—i.e., evolve and resequence (E&R)—is a powerful approach to study adaptation from standing genetic variation under controlled, replicated conditions. Nevertheless, E&R studies in Drosophila melanogaster have frequently resulted in inordinate numbers of candidate SNPs, particularly for complex traits. Here, we contrast the genomic signature of adaptation following ∼60 generations in a novel hot environment for D. melanogaster and D. simulans. For D. simulans, the regions carrying putatively selected loci were far more distinct, and thus harbored fewer false positives, than those in D. melanogaster. We propose that species without segregating inversions and higher recombination rates, such as D. simulans, are better suited for E&R studies that aim to characterize the genetic variants underlying the adaptive response. |
| first_indexed | 2024-12-22T13:57:23Z |
| format | Article |
| id | doaj.art-0b7f0c3979f143d291db75d264f0d434 |
| institution | Directory Open Access Journal |
| issn | 2160-1836 |
| language | English |
| last_indexed | 2024-12-22T13:57:23Z |
| publishDate | 2017-07-01 |
| publisher | Oxford University Press |
| record_format | Article |
| series | G3: Genes, Genomes, Genetics |
| spelling | doaj.art-0b7f0c3979f143d291db75d264f0d4342022-12-21T18:23:30ZengOxford University PressG3: Genes, Genomes, Genetics2160-18362017-07-01772337234310.1534/g3.117.04334930Drosophila simulans: A Species with Improved Resolution in Evolve and Resequence StudiesNeda BarghiRaymond ToblerViola NolteChristian SchlöttererThe combination of experimental evolution with high-throughput sequencing of pooled individuals—i.e., evolve and resequence (E&R)—is a powerful approach to study adaptation from standing genetic variation under controlled, replicated conditions. Nevertheless, E&R studies in Drosophila melanogaster have frequently resulted in inordinate numbers of candidate SNPs, particularly for complex traits. Here, we contrast the genomic signature of adaptation following ∼60 generations in a novel hot environment for D. melanogaster and D. simulans. For D. simulans, the regions carrying putatively selected loci were far more distinct, and thus harbored fewer false positives, than those in D. melanogaster. We propose that species without segregating inversions and higher recombination rates, such as D. simulans, are better suited for E&R studies that aim to characterize the genetic variants underlying the adaptive response.http://g3journal.org/lookup/doi/10.1534/g3.117.043349experimental evolutionevolve and resequenceDrosophila simulansDrosophila melanogasterchromosomal inversions |
| spellingShingle | Neda Barghi Raymond Tobler Viola Nolte Christian Schlötterer Drosophila simulans: A Species with Improved Resolution in Evolve and Resequence Studies G3: Genes, Genomes, Genetics experimental evolution evolve and resequence Drosophila simulans Drosophila melanogaster chromosomal inversions |
| title | Drosophila simulans: A Species with Improved Resolution in Evolve and Resequence Studies |
| title_full | Drosophila simulans: A Species with Improved Resolution in Evolve and Resequence Studies |
| title_fullStr | Drosophila simulans: A Species with Improved Resolution in Evolve and Resequence Studies |
| title_full_unstemmed | Drosophila simulans: A Species with Improved Resolution in Evolve and Resequence Studies |
| title_short | Drosophila simulans: A Species with Improved Resolution in Evolve and Resequence Studies |
| title_sort | drosophila simulans a species with improved resolution in evolve and resequence studies |
| topic | experimental evolution evolve and resequence Drosophila simulans Drosophila melanogaster chromosomal inversions |
| url | http://g3journal.org/lookup/doi/10.1534/g3.117.043349 |
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