Molecular differentiation of Angiostrongylus costaricensis, A. cantonensis, and A. vasorum by polymerase chain reaction- restriction fragment length polymorphism
Angiostrongylus cantonensis, A. costaricensis, and A. vasorum are etiologic agents of human parasitic diseases. Their identification, at present, is only possible by examining the adult worm after a 40-day period following infection of vertebrate hosts with the third-stage larvae. In order to obtain...
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| Format: | Article |
| Language: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
2003-01-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762003000800011 |
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| author | Caldeira Roberta L Carvalho Omar S Mendonça Cristiane LFG Graeff-Teixeira Carlos Silva Márcia CF Ben Renata Maurer Rafael Lima Walter S Lenzi Henrique L |
| author_facet | Caldeira Roberta L Carvalho Omar S Mendonça Cristiane LFG Graeff-Teixeira Carlos Silva Márcia CF Ben Renata Maurer Rafael Lima Walter S Lenzi Henrique L |
| author_sort | Caldeira Roberta L |
| collection | DOAJ |
| description | Angiostrongylus cantonensis, A. costaricensis, and A. vasorum are etiologic agents of human parasitic diseases. Their identification, at present, is only possible by examining the adult worm after a 40-day period following infection of vertebrate hosts with the third-stage larvae. In order to obtain a diagnostic tool to differentiate larvae and adult worm from the three referred species, polymerase chain reaction-restriction fragment length polymorphism was carried out. The rDNA second internal transcribed spacer (ITS2) and mtDNA cytochrome oxidase I regions were amplified, followed by digestion of fragments with the restriction enzymes RsaI, HapII, AluI, HaeIII, DdeI and ClaI. The enzymes RsaI and ClaI exhibited the most discriminating profiles for the differentiation of the regions COI of mtDNA and ITS2 of rDNA respectively. The methodology using such regions proved to be efficient for the specific differentiation of the three species of Angiostrongylus under study. |
| first_indexed | 2024-03-12T08:48:24Z |
| format | Article |
| id | doaj.art-0b81c4ccbeec44eaac2b8ee2987c845f |
| institution | Directory Open Access Journal |
| issn | 0074-0276 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T08:48:24Z |
| publishDate | 2003-01-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-0b81c4ccbeec44eaac2b8ee2987c845f2023-09-02T16:22:11ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz0074-02761678-80602003-01-0198810391043Molecular differentiation of Angiostrongylus costaricensis, A. cantonensis, and A. vasorum by polymerase chain reaction- restriction fragment length polymorphismCaldeira Roberta LCarvalho Omar SMendonça Cristiane LFGGraeff-Teixeira CarlosSilva Márcia CFBen RenataMaurer RafaelLima Walter SLenzi Henrique LAngiostrongylus cantonensis, A. costaricensis, and A. vasorum are etiologic agents of human parasitic diseases. Their identification, at present, is only possible by examining the adult worm after a 40-day period following infection of vertebrate hosts with the third-stage larvae. In order to obtain a diagnostic tool to differentiate larvae and adult worm from the three referred species, polymerase chain reaction-restriction fragment length polymorphism was carried out. The rDNA second internal transcribed spacer (ITS2) and mtDNA cytochrome oxidase I regions were amplified, followed by digestion of fragments with the restriction enzymes RsaI, HapII, AluI, HaeIII, DdeI and ClaI. The enzymes RsaI and ClaI exhibited the most discriminating profiles for the differentiation of the regions COI of mtDNA and ITS2 of rDNA respectively. The methodology using such regions proved to be efficient for the specific differentiation of the three species of Angiostrongylus under study.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762003000800011Angiostrongyluspolymerase chain reactionrestriction fragment length polymorphismsecond internal transcribed spacercytochrome oxidase I |
| spellingShingle | Caldeira Roberta L Carvalho Omar S Mendonça Cristiane LFG Graeff-Teixeira Carlos Silva Márcia CF Ben Renata Maurer Rafael Lima Walter S Lenzi Henrique L Molecular differentiation of Angiostrongylus costaricensis, A. cantonensis, and A. vasorum by polymerase chain reaction- restriction fragment length polymorphism Memorias do Instituto Oswaldo Cruz Angiostrongylus polymerase chain reaction restriction fragment length polymorphism second internal transcribed spacer cytochrome oxidase I |
| title | Molecular differentiation of Angiostrongylus costaricensis, A. cantonensis, and A. vasorum by polymerase chain reaction- restriction fragment length polymorphism |
| title_full | Molecular differentiation of Angiostrongylus costaricensis, A. cantonensis, and A. vasorum by polymerase chain reaction- restriction fragment length polymorphism |
| title_fullStr | Molecular differentiation of Angiostrongylus costaricensis, A. cantonensis, and A. vasorum by polymerase chain reaction- restriction fragment length polymorphism |
| title_full_unstemmed | Molecular differentiation of Angiostrongylus costaricensis, A. cantonensis, and A. vasorum by polymerase chain reaction- restriction fragment length polymorphism |
| title_short | Molecular differentiation of Angiostrongylus costaricensis, A. cantonensis, and A. vasorum by polymerase chain reaction- restriction fragment length polymorphism |
| title_sort | molecular differentiation of angiostrongylus costaricensis a cantonensis and a vasorum by polymerase chain reaction restriction fragment length polymorphism |
| topic | Angiostrongylus polymerase chain reaction restriction fragment length polymorphism second internal transcribed spacer cytochrome oxidase I |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762003000800011 |
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