The Inhibition Activities of the Fruit Extract of <i>Plinia cauliflora</i> against Melanoma Cells and the Single-Stranded DNA-Binding Protein (SSB) from <i>Klebsiella pneumoniae</i>
<i>Plinia cauliflora</i> has been associated with numerous ethnobotanical applications. In this study, we uncovered that the fruit extract of <i>P. cauliflora</i>, obtained using 50% ethanol, possesses inhibition activity against the <i>Klebsiella pneumoniae</i> s...
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MDPI AG
2023-10-01
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| Series: | Applied Sciences |
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| Online Access: | https://www.mdpi.com/2076-3417/13/19/11061 |
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| author | En-Shyh Lin Cheng-Yang Huang |
| author_facet | En-Shyh Lin Cheng-Yang Huang |
| author_sort | En-Shyh Lin |
| collection | DOAJ |
| description | <i>Plinia cauliflora</i> has been associated with numerous ethnobotanical applications. In this study, we uncovered that the fruit extract of <i>P. cauliflora</i>, obtained using 50% ethanol, possesses inhibition activity against the <i>Klebsiella pneumoniae</i> single-stranded DNA-binding protein (KpSSB). SSB plays a critical role in cell survival, making it an attractive target for the development of anti-infective drugs. The inhibition activity against KpSSB by the <i>P. cauliflora</i> extract demonstrated an IC<sub>50</sub> value of 73 ± 8 μg/mL. By using gas chromatography–mass spectrometry, the chemical content of this extract was tentatively determined. The top 15 compounds (>0.7%) were as follows: 5-hydroxymethylfurfural, 2,3-dihydro-3,5-dihydroxy-6-methyl-4h-pyran-4-one, 2,5-diformylfuran, furfural, ace-tic acid, citraconic anhydride, formic acid, ethyl 4-hydroxy-3-methylbut-2-enoate, furfuryl alcohol, furyl hydroxymethyl ketone, 3-acetyl-3-hydroxyoxolane-2-one, 2,3-dihydro-5-hydroxy-2-(hydroxymethyl)-4H-pyran-4-one, 2(3H)-furanone, dihy-dro-4-hydroxy-, hydroxyacetone, and 1-hydroxybut-3-en-2-one. To analyze the possible binding modes, the three most abundant compounds were then subjected to docking analysis. We also investigated whether the <i>P. cauliflora</i> extract exhibited any cytotoxic and antiproliferative effects on the survival of B16F10 melanoma cells. Additionally, we found that the extract of <i>P. cauliflora</i> could inhibit the migration and induce apoptosis of B16F10 cells. The results of this study collectively suggest that <i>P. cauliflora</i> holds potential pharmacological benefits, warranting further exploration for therapeutic applications. |
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| last_indexed | 2024-03-10T21:48:14Z |
| publishDate | 2023-10-01 |
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| series | Applied Sciences |
| spelling | doaj.art-0b82dc49851e409888d1dde7a54918fb2023-11-19T14:08:06ZengMDPI AGApplied Sciences2076-34172023-10-0113191106110.3390/app131911061The Inhibition Activities of the Fruit Extract of <i>Plinia cauliflora</i> against Melanoma Cells and the Single-Stranded DNA-Binding Protein (SSB) from <i>Klebsiella pneumoniae</i>En-Shyh Lin0Cheng-Yang Huang1Department of Beauty Science, National Taichung University of Science and Technology, Taichung City 403, TaiwanDepartment of Biomedical Sciences, Chung Shan Medical University, Taichung City 402, Taiwan<i>Plinia cauliflora</i> has been associated with numerous ethnobotanical applications. In this study, we uncovered that the fruit extract of <i>P. cauliflora</i>, obtained using 50% ethanol, possesses inhibition activity against the <i>Klebsiella pneumoniae</i> single-stranded DNA-binding protein (KpSSB). SSB plays a critical role in cell survival, making it an attractive target for the development of anti-infective drugs. The inhibition activity against KpSSB by the <i>P. cauliflora</i> extract demonstrated an IC<sub>50</sub> value of 73 ± 8 μg/mL. By using gas chromatography–mass spectrometry, the chemical content of this extract was tentatively determined. The top 15 compounds (>0.7%) were as follows: 5-hydroxymethylfurfural, 2,3-dihydro-3,5-dihydroxy-6-methyl-4h-pyran-4-one, 2,5-diformylfuran, furfural, ace-tic acid, citraconic anhydride, formic acid, ethyl 4-hydroxy-3-methylbut-2-enoate, furfuryl alcohol, furyl hydroxymethyl ketone, 3-acetyl-3-hydroxyoxolane-2-one, 2,3-dihydro-5-hydroxy-2-(hydroxymethyl)-4H-pyran-4-one, 2(3H)-furanone, dihy-dro-4-hydroxy-, hydroxyacetone, and 1-hydroxybut-3-en-2-one. To analyze the possible binding modes, the three most abundant compounds were then subjected to docking analysis. We also investigated whether the <i>P. cauliflora</i> extract exhibited any cytotoxic and antiproliferative effects on the survival of B16F10 melanoma cells. Additionally, we found that the extract of <i>P. cauliflora</i> could inhibit the migration and induce apoptosis of B16F10 cells. The results of this study collectively suggest that <i>P. cauliflora</i> holds potential pharmacological benefits, warranting further exploration for therapeutic applications.https://www.mdpi.com/2076-3417/13/19/11061<i>Plinia cauliflora</i>SSBsingle-stranded DNAantipathogenB16F10 melanomaGC–MS analysis |
| spellingShingle | En-Shyh Lin Cheng-Yang Huang The Inhibition Activities of the Fruit Extract of <i>Plinia cauliflora</i> against Melanoma Cells and the Single-Stranded DNA-Binding Protein (SSB) from <i>Klebsiella pneumoniae</i> Applied Sciences <i>Plinia cauliflora</i> SSB single-stranded DNA antipathogen B16F10 melanoma GC–MS analysis |
| title | The Inhibition Activities of the Fruit Extract of <i>Plinia cauliflora</i> against Melanoma Cells and the Single-Stranded DNA-Binding Protein (SSB) from <i>Klebsiella pneumoniae</i> |
| title_full | The Inhibition Activities of the Fruit Extract of <i>Plinia cauliflora</i> against Melanoma Cells and the Single-Stranded DNA-Binding Protein (SSB) from <i>Klebsiella pneumoniae</i> |
| title_fullStr | The Inhibition Activities of the Fruit Extract of <i>Plinia cauliflora</i> against Melanoma Cells and the Single-Stranded DNA-Binding Protein (SSB) from <i>Klebsiella pneumoniae</i> |
| title_full_unstemmed | The Inhibition Activities of the Fruit Extract of <i>Plinia cauliflora</i> against Melanoma Cells and the Single-Stranded DNA-Binding Protein (SSB) from <i>Klebsiella pneumoniae</i> |
| title_short | The Inhibition Activities of the Fruit Extract of <i>Plinia cauliflora</i> against Melanoma Cells and the Single-Stranded DNA-Binding Protein (SSB) from <i>Klebsiella pneumoniae</i> |
| title_sort | inhibition activities of the fruit extract of i plinia cauliflora i against melanoma cells and the single stranded dna binding protein ssb from i klebsiella pneumoniae i |
| topic | <i>Plinia cauliflora</i> SSB single-stranded DNA antipathogen B16F10 melanoma GC–MS analysis |
| url | https://www.mdpi.com/2076-3417/13/19/11061 |
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