Effect of cyclic fatty acid monomers on fat absorption and transport depends on their positioning within the ingested triacylglycerols
We investigated the intestinal digestion of cyclic fatty acid monomers (CFAM) isolated from heated linseed oil and their effects upon fatty acid lymphatic transport and lipoprotein profile in lymph. These cyclic fatty acid monomers were acylated in specific positions in the glycerol backbone of tria...
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| Format: | Article |
| Language: | English |
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Elsevier
1997-08-01
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| Series: | Journal of Lipid Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520371856 |
| _version_ | 1818607411419676672 |
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| author | J C Martin C Caselli S Broquet P Juanéda M Nour J L Sébédio A Bernard |
| author_facet | J C Martin C Caselli S Broquet P Juanéda M Nour J L Sébédio A Bernard |
| author_sort | J C Martin |
| collection | DOAJ |
| description | We investigated the intestinal digestion of cyclic fatty acid monomers (CFAM) isolated from heated linseed oil and their effects upon fatty acid lymphatic transport and lipoprotein profile in lymph. These cyclic fatty acid monomers were acylated in specific positions in the glycerol backbone of triacylglycerols (sn-(1/3) position for the 1C oil, sn-2 position for the 2C oil and together in the sn-1,2, and 3 positions for the 3C oil) and administered intragastrically to lymph-canulated rats. Their lumenal digestibility was also assessed in vitro using a pancreatic lipase assay. The lipase activity was 1.9 to 6.6 less towards the triacylglycerols acylated with cyclic fatty acids compared to control. The lowest activity was with the 2C oil. In the hydrolytic products, the cyclic fatty acid contents were similar between the experimental groups. When absorbed as 2-monoacyl-sn-glycerol (2C oil), cyclic fatty acid monomers were better and unselectively recovered into the lymph than when absorbed as free fatty acids (1C oil). In that latter situation, the bulkier cyclic fatty acids (C6 and cis membered-ring CFAM) were transported into the lymph to a lesser extent. The appearance of the lymphatic chylomicrons was delayed in rats fed the 1C oil. Cyclic fatty acid monomers from the 2C oil only increased the lymphatic transport of saturated fatty acids (80%). Cyclic fatty acids from the 3C oil (absorbed as 2-monoacyl-sn-glycerol and free fatty acid) usually elicited intermediary effects. We conclude that the effects of cyclic fatty acid monomers upon the intestinal metabolism are greatly influenced by their positioning within the triacylglycerol and that the structure of the cyclic fatty acids influences their lymphatic recovery only when they are absorbed as free fatty acid. |
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| format | Article |
| id | doaj.art-0b840483ec554a5b9e12206c1df4bc90 |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-16T14:26:19Z |
| publishDate | 1997-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-0b840483ec554a5b9e12206c1df4bc902022-12-21T22:28:22ZengElsevierJournal of Lipid Research0022-22751997-08-0138816661679Effect of cyclic fatty acid monomers on fat absorption and transport depends on their positioning within the ingested triacylglycerolsJ C Martin0C Caselli1S Broquet2P Juanéda3M Nour4J L Sébédio5A Bernard6Unité de Nutrition Lipidique, Institut National de la Recherche Agronomique, Dijon, France.Unité de Nutrition Lipidique, Institut National de la Recherche Agronomique, Dijon, France.Unité de Nutrition Lipidique, Institut National de la Recherche Agronomique, Dijon, France.Unité de Nutrition Lipidique, Institut National de la Recherche Agronomique, Dijon, France.Unité de Nutrition Lipidique, Institut National de la Recherche Agronomique, Dijon, France.Unité de Nutrition Lipidique, Institut National de la Recherche Agronomique, Dijon, France.Unité de Nutrition Lipidique, Institut National de la Recherche Agronomique, Dijon, France.We investigated the intestinal digestion of cyclic fatty acid monomers (CFAM) isolated from heated linseed oil and their effects upon fatty acid lymphatic transport and lipoprotein profile in lymph. These cyclic fatty acid monomers were acylated in specific positions in the glycerol backbone of triacylglycerols (sn-(1/3) position for the 1C oil, sn-2 position for the 2C oil and together in the sn-1,2, and 3 positions for the 3C oil) and administered intragastrically to lymph-canulated rats. Their lumenal digestibility was also assessed in vitro using a pancreatic lipase assay. The lipase activity was 1.9 to 6.6 less towards the triacylglycerols acylated with cyclic fatty acids compared to control. The lowest activity was with the 2C oil. In the hydrolytic products, the cyclic fatty acid contents were similar between the experimental groups. When absorbed as 2-monoacyl-sn-glycerol (2C oil), cyclic fatty acid monomers were better and unselectively recovered into the lymph than when absorbed as free fatty acids (1C oil). In that latter situation, the bulkier cyclic fatty acids (C6 and cis membered-ring CFAM) were transported into the lymph to a lesser extent. The appearance of the lymphatic chylomicrons was delayed in rats fed the 1C oil. Cyclic fatty acid monomers from the 2C oil only increased the lymphatic transport of saturated fatty acids (80%). Cyclic fatty acids from the 3C oil (absorbed as 2-monoacyl-sn-glycerol and free fatty acid) usually elicited intermediary effects. We conclude that the effects of cyclic fatty acid monomers upon the intestinal metabolism are greatly influenced by their positioning within the triacylglycerol and that the structure of the cyclic fatty acids influences their lymphatic recovery only when they are absorbed as free fatty acid.http://www.sciencedirect.com/science/article/pii/S0022227520371856 |
| spellingShingle | J C Martin C Caselli S Broquet P Juanéda M Nour J L Sébédio A Bernard Effect of cyclic fatty acid monomers on fat absorption and transport depends on their positioning within the ingested triacylglycerols Journal of Lipid Research |
| title | Effect of cyclic fatty acid monomers on fat absorption and transport depends on their positioning within the ingested triacylglycerols |
| title_full | Effect of cyclic fatty acid monomers on fat absorption and transport depends on their positioning within the ingested triacylglycerols |
| title_fullStr | Effect of cyclic fatty acid monomers on fat absorption and transport depends on their positioning within the ingested triacylglycerols |
| title_full_unstemmed | Effect of cyclic fatty acid monomers on fat absorption and transport depends on their positioning within the ingested triacylglycerols |
| title_short | Effect of cyclic fatty acid monomers on fat absorption and transport depends on their positioning within the ingested triacylglycerols |
| title_sort | effect of cyclic fatty acid monomers on fat absorption and transport depends on their positioning within the ingested triacylglycerols |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520371856 |
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