Assessment of quantitative and semi-quantitative biological test methods of artesunate in vitro
Artesunate is the current most potent antimalarial drug widely used for the treatment of malaria. Considering the emergence of artemisinin resistance, several situations may require a simple method for artesunate quantification. We thus developed a quantitative and a semi-quantitative biological met...
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| Format: | Article |
| Language: | English |
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EDP Sciences
2022-01-01
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| Series: | Parasite |
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| Online Access: | https://www.parasite-journal.org/articles/parasite/full_html/2022/01/parasite210129/parasite210129.html |
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| author | Kouakou Yobouet Ines Omorou Roukayatou Said Ibrahim Bin Lavoignat Adeline Bonnot Guillaume Bienvenu Anne-Lise Picot Stéphane |
| author_facet | Kouakou Yobouet Ines Omorou Roukayatou Said Ibrahim Bin Lavoignat Adeline Bonnot Guillaume Bienvenu Anne-Lise Picot Stéphane |
| author_sort | Kouakou Yobouet Ines |
| collection | DOAJ |
| description | Artesunate is the current most potent antimalarial drug widely used for the treatment of malaria. Considering the emergence of artemisinin resistance, several situations may require a simple method for artesunate quantification. We thus developed a quantitative and a semi-quantitative biological method for the determination of artesunate in liquid samples. The tests are based on the measurement of samples’ antimalarial activity on Plasmodium falciparum 3D7 using a modified SYBR Green I drug susceptibility test. For the quantitative test, we established a standard curve that resulted from a dose–response curve and evaluated its performances using controls samples. Whereas the linear regression analysis between artesunate concentration and antimalarial activity showed promising results (linearity range 1.5–24.6 ng/mL, r2 = 0.9373), we found that artesunate content of the controls was significantly overestimated (p = 0.0313). For the semi-quantitative test, we compared the antimalarial activities of samples collected during permeation studies of artesunate to that of a reference (artesunate IC50) by statistical analysis. We demonstrated that antimalarial activities of samples from permeation tests using a powder formulation of artesunate were greater than those of samples from tests using a solution formulation. Bioassays can be simple techniques to assess artesunate in liquid samples, particularly in resource-limited settings. Comparison with reference methods is still recommended when accurate drug quantification is required. |
| first_indexed | 2024-03-09T08:59:48Z |
| format | Article |
| id | doaj.art-0b84b16369544d13a730c2797cbabd37 |
| institution | Directory Open Access Journal |
| issn | 1776-1042 |
| language | English |
| last_indexed | 2024-03-09T08:59:48Z |
| publishDate | 2022-01-01 |
| publisher | EDP Sciences |
| record_format | Article |
| series | Parasite |
| spelling | doaj.art-0b84b16369544d13a730c2797cbabd372023-12-02T11:48:03ZengEDP SciencesParasite1776-10422022-01-01291810.1051/parasite/2022019parasite210129Assessment of quantitative and semi-quantitative biological test methods of artesunate in vitroKouakou Yobouet Ines0Omorou Roukayatou1Said Ibrahim Bin2Lavoignat Adeline3Bonnot Guillaume4Bienvenu Anne-Lise5Picot Stéphane6Université de Lyon, Malaria Research Unit, ICBMS, UMR 5246 CNRS-INSA-CPE-Université Lyon1Université de Lyon, Malaria Research Unit, ICBMS, UMR 5246 CNRS-INSA-CPE-Université Lyon1Université de Lyon, Malaria Research Unit, ICBMS, UMR 5246 CNRS-INSA-CPE-Université Lyon1Université de Lyon, Malaria Research Unit, ICBMS, UMR 5246 CNRS-INSA-CPE-Université Lyon1Université de Lyon, Malaria Research Unit, ICBMS, UMR 5246 CNRS-INSA-CPE-Université Lyon1Université de Lyon, Malaria Research Unit, ICBMS, UMR 5246 CNRS-INSA-CPE-Université Lyon1Université de Lyon, Malaria Research Unit, ICBMS, UMR 5246 CNRS-INSA-CPE-Université Lyon1Artesunate is the current most potent antimalarial drug widely used for the treatment of malaria. Considering the emergence of artemisinin resistance, several situations may require a simple method for artesunate quantification. We thus developed a quantitative and a semi-quantitative biological method for the determination of artesunate in liquid samples. The tests are based on the measurement of samples’ antimalarial activity on Plasmodium falciparum 3D7 using a modified SYBR Green I drug susceptibility test. For the quantitative test, we established a standard curve that resulted from a dose–response curve and evaluated its performances using controls samples. Whereas the linear regression analysis between artesunate concentration and antimalarial activity showed promising results (linearity range 1.5–24.6 ng/mL, r2 = 0.9373), we found that artesunate content of the controls was significantly overestimated (p = 0.0313). For the semi-quantitative test, we compared the antimalarial activities of samples collected during permeation studies of artesunate to that of a reference (artesunate IC50) by statistical analysis. We demonstrated that antimalarial activities of samples from permeation tests using a powder formulation of artesunate were greater than those of samples from tests using a solution formulation. Bioassays can be simple techniques to assess artesunate in liquid samples, particularly in resource-limited settings. Comparison with reference methods is still recommended when accurate drug quantification is required.https://www.parasite-journal.org/articles/parasite/full_html/2022/01/parasite210129/parasite210129.htmlartesunatemalariabioassayquantitative testsemi-quantitative test |
| spellingShingle | Kouakou Yobouet Ines Omorou Roukayatou Said Ibrahim Bin Lavoignat Adeline Bonnot Guillaume Bienvenu Anne-Lise Picot Stéphane Assessment of quantitative and semi-quantitative biological test methods of artesunate in vitro Parasite artesunate malaria bioassay quantitative test semi-quantitative test |
| title | Assessment of quantitative and semi-quantitative biological test methods of artesunate in vitro |
| title_full | Assessment of quantitative and semi-quantitative biological test methods of artesunate in vitro |
| title_fullStr | Assessment of quantitative and semi-quantitative biological test methods of artesunate in vitro |
| title_full_unstemmed | Assessment of quantitative and semi-quantitative biological test methods of artesunate in vitro |
| title_short | Assessment of quantitative and semi-quantitative biological test methods of artesunate in vitro |
| title_sort | assessment of quantitative and semi quantitative biological test methods of artesunate in vitro |
| topic | artesunate malaria bioassay quantitative test semi-quantitative test |
| url | https://www.parasite-journal.org/articles/parasite/full_html/2022/01/parasite210129/parasite210129.html |
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