STUDY ON IN VITRO PROPAGATION AND DEVELOPMENT GROWTH OF GYNOSTEMMA PUBESCENS IN THE GREENHOUSE CONDITION
The Gynostemma pubescens plant, which is distributed mainly over the northern provinces, is a valuable and beneficial herb for human health. In this study, we investigated in vitro propagation and cultivation to evaluate the growth and development of Gynostemma pubescens in greenhouse conditions at...
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| Format: | Article |
| Language: | English |
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Dalat University
2018-09-01
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| Series: | Tạp chí Khoa học Đại học Đà Lạt |
| Subjects: | |
| Online Access: | http://tckh.dlu.edu.vn/index.php/tckhdhdl/article/view/506 |
| _version_ | 1818190814094819328 |
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| author | Nguyễn Thị Thanh Hằng Lê Ái Vân Đinh Văn Khiêm Hoàng Văn Cương Nguyễn Thị Phượng Hoàng Phan Xuân Huyên |
| author_facet | Nguyễn Thị Thanh Hằng Lê Ái Vân Đinh Văn Khiêm Hoàng Văn Cương Nguyễn Thị Phượng Hoàng Phan Xuân Huyên |
| author_sort | Nguyễn Thị Thanh Hằng |
| collection | DOAJ |
| description | The Gynostemma pubescens plant, which is distributed mainly over the northern provinces, is a valuable and beneficial herb for human health. In this study, we investigated in vitro propagation and cultivation to evaluate the growth and development of Gynostemma pubescens in greenhouse conditions at Dalat city, Lamdong province. The results showed that MS medium supplemented with 1mg/l BA, 30 g/l sucrose, 8g/l agar, pH 5.8 was the best medium for shoot regeneration in vitro, with a shoot height of 1.84cm, and 10.50 shoots/explant. The MS medium contained 0.5mg/l TDZ, 30g/l sucrose, 8g/l agar, pH 5.8 was the most suitable medium for shoot regeneration in vitro, with a shoot height of 1.99cm, and 13.80 shoots/explant. Concentrations of IBA from 0 to 1 mg/l were appropriate for in vitro root regeneration, yielding a root regeneration rate of 100%. Coconut fiber powder was the most suitable substrate to transfer the plantlets to the greenhouse, with a survival rate of 100%, plant height of 9.73cm, and root length of 6.45cm. Humus proved the best substrate for plant development and growth, with a survival rate of 100%, plant height of 82.08cm, root length of 36.57cm and a fresh weight of 57.32g/plant. Watering with 100 ml of Nitrophoska fertilizer (2g/l) once a week was the best for plant growth, with a survival rate of 100%, height of 94.22cm, root length of 37.71cm, and a fresh weight of 59.38g/plant. |
| first_indexed | 2024-12-12T00:04:41Z |
| format | Article |
| id | doaj.art-0b888d1370c84e6780a5a916ffb47589 |
| institution | Directory Open Access Journal |
| issn | 0866-787X 0866-787X |
| language | English |
| last_indexed | 2024-12-12T00:04:41Z |
| publishDate | 2018-09-01 |
| publisher | Dalat University |
| record_format | Article |
| series | Tạp chí Khoa học Đại học Đà Lạt |
| spelling | doaj.art-0b888d1370c84e6780a5a916ffb475892022-12-22T00:45:09ZengDalat UniversityTạp chí Khoa học Đại học Đà Lạt0866-787X0866-787X2018-09-01839911210.37569/DalatUniversity.8.3.506(2018)261STUDY ON IN VITRO PROPAGATION AND DEVELOPMENT GROWTH OF GYNOSTEMMA PUBESCENS IN THE GREENHOUSE CONDITIONNguyễn Thị Thanh Hằng0Lê Ái Vân1Đinh Văn Khiêm2Hoàng Văn Cương3Nguyễn Thị Phượng Hoàng4Phan Xuân Huyên5Viện Nghiên cứu Khoa học Tây NguyênViện Nghiên cứu Khoa học Tây NguyênViện Nghiên cứu Khoa học Tây NguyênViện Nghiên cứu Khoa học Tây NguyênViện Nghiên cứu Khoa học Tây NguyênViện Nghiên cứu Khoa học Tây NguyênThe Gynostemma pubescens plant, which is distributed mainly over the northern provinces, is a valuable and beneficial herb for human health. In this study, we investigated in vitro propagation and cultivation to evaluate the growth and development of Gynostemma pubescens in greenhouse conditions at Dalat city, Lamdong province. The results showed that MS medium supplemented with 1mg/l BA, 30 g/l sucrose, 8g/l agar, pH 5.8 was the best medium for shoot regeneration in vitro, with a shoot height of 1.84cm, and 10.50 shoots/explant. The MS medium contained 0.5mg/l TDZ, 30g/l sucrose, 8g/l agar, pH 5.8 was the most suitable medium for shoot regeneration in vitro, with a shoot height of 1.99cm, and 13.80 shoots/explant. Concentrations of IBA from 0 to 1 mg/l were appropriate for in vitro root regeneration, yielding a root regeneration rate of 100%. Coconut fiber powder was the most suitable substrate to transfer the plantlets to the greenhouse, with a survival rate of 100%, plant height of 9.73cm, and root length of 6.45cm. Humus proved the best substrate for plant development and growth, with a survival rate of 100%, plant height of 82.08cm, root length of 36.57cm and a fresh weight of 57.32g/plant. Watering with 100 ml of Nitrophoska fertilizer (2g/l) once a week was the best for plant growth, with a survival rate of 100%, height of 94.22cm, root length of 37.71cm, and a fresh weight of 59.38g/plant.http://tckh.dlu.edu.vn/index.php/tckhdhdl/article/view/506chiều cao câychiều dài rễcây giảo cổ lamsinh trưởng phát triểnsố chồi. |
| spellingShingle | Nguyễn Thị Thanh Hằng Lê Ái Vân Đinh Văn Khiêm Hoàng Văn Cương Nguyễn Thị Phượng Hoàng Phan Xuân Huyên STUDY ON IN VITRO PROPAGATION AND DEVELOPMENT GROWTH OF GYNOSTEMMA PUBESCENS IN THE GREENHOUSE CONDITION Tạp chí Khoa học Đại học Đà Lạt chiều cao cây chiều dài rễ cây giảo cổ lam sinh trưởng phát triển số chồi. |
| title | STUDY ON IN VITRO PROPAGATION AND DEVELOPMENT GROWTH OF GYNOSTEMMA PUBESCENS IN THE GREENHOUSE CONDITION |
| title_full | STUDY ON IN VITRO PROPAGATION AND DEVELOPMENT GROWTH OF GYNOSTEMMA PUBESCENS IN THE GREENHOUSE CONDITION |
| title_fullStr | STUDY ON IN VITRO PROPAGATION AND DEVELOPMENT GROWTH OF GYNOSTEMMA PUBESCENS IN THE GREENHOUSE CONDITION |
| title_full_unstemmed | STUDY ON IN VITRO PROPAGATION AND DEVELOPMENT GROWTH OF GYNOSTEMMA PUBESCENS IN THE GREENHOUSE CONDITION |
| title_short | STUDY ON IN VITRO PROPAGATION AND DEVELOPMENT GROWTH OF GYNOSTEMMA PUBESCENS IN THE GREENHOUSE CONDITION |
| title_sort | study on in vitro propagation and development growth of gynostemma pubescens in the greenhouse condition |
| topic | chiều cao cây chiều dài rễ cây giảo cổ lam sinh trưởng phát triển số chồi. |
| url | http://tckh.dlu.edu.vn/index.php/tckhdhdl/article/view/506 |
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