Improvement of steatotic rat liver function with a defatting cocktail during ex situ normothermic machine perfusion is not directly related to liver fat content.
There is a significant organ shortage in the field of liver transplantation, partly due to a high discard rate of steatotic livers from donors. These organs are known to function poorly if transplanted but make up a significant portion of the available pool of donated livers. This study demonstrates...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2020-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0232886 |
| _version_ | 1828113369713868800 |
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| author | Siavash Raigani Cailah Carroll Stephanie Griffith Casie Pendexter Ivy Rosales Hany Deirawan Rafic Beydoun Martin Yarmush Korkut Uygun Heidi Yeh |
| author_facet | Siavash Raigani Cailah Carroll Stephanie Griffith Casie Pendexter Ivy Rosales Hany Deirawan Rafic Beydoun Martin Yarmush Korkut Uygun Heidi Yeh |
| author_sort | Siavash Raigani |
| collection | DOAJ |
| description | There is a significant organ shortage in the field of liver transplantation, partly due to a high discard rate of steatotic livers from donors. These organs are known to function poorly if transplanted but make up a significant portion of the available pool of donated livers. This study demonstrates the ability to improve the function of steatotic rat livers using a combination of ex situ machine perfusion and a "defatting" drug cocktail. After 6 hours of perfusion, defatted livers demonstrated lower perfusate lactate levels and improved bile quality as demonstrated by higher bile bicarbonate and lower bile lactate. Furthermore, defatting was associated with decreased gene expression of pro-inflammatory cytokines and increased expression of enzymes involved in mitochondrial fatty acid oxidation. Rehabilitation of marginal or discarded steatotic livers using machine perfusion and tailored drug therapy can significantly increase the supply of donor livers for transplantation. |
| first_indexed | 2024-04-11T12:04:44Z |
| format | Article |
| id | doaj.art-0b8a2309074841ab99d5ecb4cf16959a |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-11T12:04:44Z |
| publishDate | 2020-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-0b8a2309074841ab99d5ecb4cf16959a2022-12-22T04:24:44ZengPublic Library of Science (PLoS)PLoS ONE1932-62032020-01-01155e023288610.1371/journal.pone.0232886Improvement of steatotic rat liver function with a defatting cocktail during ex situ normothermic machine perfusion is not directly related to liver fat content.Siavash RaiganiCailah CarrollStephanie GriffithCasie PendexterIvy RosalesHany DeirawanRafic BeydounMartin YarmushKorkut UygunHeidi YehThere is a significant organ shortage in the field of liver transplantation, partly due to a high discard rate of steatotic livers from donors. These organs are known to function poorly if transplanted but make up a significant portion of the available pool of donated livers. This study demonstrates the ability to improve the function of steatotic rat livers using a combination of ex situ machine perfusion and a "defatting" drug cocktail. After 6 hours of perfusion, defatted livers demonstrated lower perfusate lactate levels and improved bile quality as demonstrated by higher bile bicarbonate and lower bile lactate. Furthermore, defatting was associated with decreased gene expression of pro-inflammatory cytokines and increased expression of enzymes involved in mitochondrial fatty acid oxidation. Rehabilitation of marginal or discarded steatotic livers using machine perfusion and tailored drug therapy can significantly increase the supply of donor livers for transplantation.https://doi.org/10.1371/journal.pone.0232886 |
| spellingShingle | Siavash Raigani Cailah Carroll Stephanie Griffith Casie Pendexter Ivy Rosales Hany Deirawan Rafic Beydoun Martin Yarmush Korkut Uygun Heidi Yeh Improvement of steatotic rat liver function with a defatting cocktail during ex situ normothermic machine perfusion is not directly related to liver fat content. PLoS ONE |
| title | Improvement of steatotic rat liver function with a defatting cocktail during ex situ normothermic machine perfusion is not directly related to liver fat content. |
| title_full | Improvement of steatotic rat liver function with a defatting cocktail during ex situ normothermic machine perfusion is not directly related to liver fat content. |
| title_fullStr | Improvement of steatotic rat liver function with a defatting cocktail during ex situ normothermic machine perfusion is not directly related to liver fat content. |
| title_full_unstemmed | Improvement of steatotic rat liver function with a defatting cocktail during ex situ normothermic machine perfusion is not directly related to liver fat content. |
| title_short | Improvement of steatotic rat liver function with a defatting cocktail during ex situ normothermic machine perfusion is not directly related to liver fat content. |
| title_sort | improvement of steatotic rat liver function with a defatting cocktail during ex situ normothermic machine perfusion is not directly related to liver fat content |
| url | https://doi.org/10.1371/journal.pone.0232886 |
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