Correlation of Cell Proliferation with Surface Properties of Polymer-like Carbon Films of Different Thicknesses Prepared by a Radio-Frequency Plasma CVD Process
In this study, correlation of cell proliferation with surface properties of the polymer-like carbon (PLC) films of different thicknesses prepared by radio-frequency plasma CVD are investigated. Four PLC samples were prepared via radio frequency plasma chemical vapor deposition on Si substrates. Each...
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MDPI AG
2022-06-01
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| Series: | Materials |
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| Online Access: | https://www.mdpi.com/1996-1944/15/13/4466 |
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| author | Kazuya Kanasugi Hiroaki Eguchi Yasuharu Ohgoe Yoshinobu Manome Ali Alanazi Kenji Hirakuri |
| author_facet | Kazuya Kanasugi Hiroaki Eguchi Yasuharu Ohgoe Yoshinobu Manome Ali Alanazi Kenji Hirakuri |
| author_sort | Kazuya Kanasugi |
| collection | DOAJ |
| description | In this study, correlation of cell proliferation with surface properties of the polymer-like carbon (PLC) films of different thicknesses prepared by radio-frequency plasma CVD are investigated. Four PLC samples were prepared via radio frequency plasma chemical vapor deposition on Si substrates. Each PLC film was analyzed using spectroscopic ellipsometry to determine its thickness, refractive index (n), and extinction coefficient (k); the thickness ranged from 29.0 to 356.5 nm. Based on their n–k plots, all the samples were classified as PLC-type films. The biological response of the PLC films was evaluated in vitro using a cell culture. The samples with relatively thick PLC films (>300 nm) exhibited stronger cell proliferation properties than those with thinner films. Moreover, the results of the surface analysis showed no significant differences in the surface composition of those PLC samples, as analyzed using X-ray photoelectron spectroscopy, but that as the PLC films became thicker, their surfaces became rougher on the nanoscale and their wettability improved. Overall, this study showed that careful control of the film growth of PLC films, which affects their surface properties, is essential for their use in bio-interface applications. |
| first_indexed | 2024-03-09T04:03:10Z |
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| id | doaj.art-0b8d83a4ac6f4ac08ca6f13f79da6cce |
| institution | Directory Open Access Journal |
| issn | 1996-1944 |
| language | English |
| last_indexed | 2024-03-09T04:03:10Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Materials |
| spelling | doaj.art-0b8d83a4ac6f4ac08ca6f13f79da6cce2023-12-03T14:09:56ZengMDPI AGMaterials1996-19442022-06-011513446610.3390/ma15134466Correlation of Cell Proliferation with Surface Properties of Polymer-like Carbon Films of Different Thicknesses Prepared by a Radio-Frequency Plasma CVD ProcessKazuya Kanasugi0Hiroaki Eguchi1Yasuharu Ohgoe2Yoshinobu Manome3Ali Alanazi4Kenji Hirakuri5Department of Electrical and Electronic Engineering, Faculty of Engineering, Tokyo Denki University, 5 Senju Asahi-cho, Adachi-ku, Tokyo 120-8551, JapanDepartment of Electrical and Electronic Engineering, Faculty of Engineering, Tokyo Denki University, 5 Senju Asahi-cho, Adachi-ku, Tokyo 120-8551, JapanDivision of Electronic Engineering, Faculty of Science and Engineering, Tokyo Denki University, Ishizaka Hatoyama, Saitama 350-0394, JapanCore Research Facilities, The Jikei University School of Medicine, 3-25-8, Nishi-shinbashi, Minato-ku, Tokyo 105-8461, JapanApplied Medical Sciences College, King Saud University, Riyadh 11451, Saudi ArabiaDepartment of Electrical and Electronic Engineering, Faculty of Engineering, Tokyo Denki University, 5 Senju Asahi-cho, Adachi-ku, Tokyo 120-8551, JapanIn this study, correlation of cell proliferation with surface properties of the polymer-like carbon (PLC) films of different thicknesses prepared by radio-frequency plasma CVD are investigated. Four PLC samples were prepared via radio frequency plasma chemical vapor deposition on Si substrates. Each PLC film was analyzed using spectroscopic ellipsometry to determine its thickness, refractive index (n), and extinction coefficient (k); the thickness ranged from 29.0 to 356.5 nm. Based on their n–k plots, all the samples were classified as PLC-type films. The biological response of the PLC films was evaluated in vitro using a cell culture. The samples with relatively thick PLC films (>300 nm) exhibited stronger cell proliferation properties than those with thinner films. Moreover, the results of the surface analysis showed no significant differences in the surface composition of those PLC samples, as analyzed using X-ray photoelectron spectroscopy, but that as the PLC films became thicker, their surfaces became rougher on the nanoscale and their wettability improved. Overall, this study showed that careful control of the film growth of PLC films, which affects their surface properties, is essential for their use in bio-interface applications.https://www.mdpi.com/1996-1944/15/13/4466cell proliferationpolymer-like carbon filmsoptical classificationfilm thickness |
| spellingShingle | Kazuya Kanasugi Hiroaki Eguchi Yasuharu Ohgoe Yoshinobu Manome Ali Alanazi Kenji Hirakuri Correlation of Cell Proliferation with Surface Properties of Polymer-like Carbon Films of Different Thicknesses Prepared by a Radio-Frequency Plasma CVD Process Materials cell proliferation polymer-like carbon films optical classification film thickness |
| title | Correlation of Cell Proliferation with Surface Properties of Polymer-like Carbon Films of Different Thicknesses Prepared by a Radio-Frequency Plasma CVD Process |
| title_full | Correlation of Cell Proliferation with Surface Properties of Polymer-like Carbon Films of Different Thicknesses Prepared by a Radio-Frequency Plasma CVD Process |
| title_fullStr | Correlation of Cell Proliferation with Surface Properties of Polymer-like Carbon Films of Different Thicknesses Prepared by a Radio-Frequency Plasma CVD Process |
| title_full_unstemmed | Correlation of Cell Proliferation with Surface Properties of Polymer-like Carbon Films of Different Thicknesses Prepared by a Radio-Frequency Plasma CVD Process |
| title_short | Correlation of Cell Proliferation with Surface Properties of Polymer-like Carbon Films of Different Thicknesses Prepared by a Radio-Frequency Plasma CVD Process |
| title_sort | correlation of cell proliferation with surface properties of polymer like carbon films of different thicknesses prepared by a radio frequency plasma cvd process |
| topic | cell proliferation polymer-like carbon films optical classification film thickness |
| url | https://www.mdpi.com/1996-1944/15/13/4466 |
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