First Report of <italic toggle="yes">bla</italic><sub>VIM-4</sub>- and <italic toggle="yes">mcr-9</italic>-Coharboring <italic toggle="yes">Enterobacter</italic> Species Isolated from a Pediatric Patient
ABSTRACT An Enterobacter hormaechei isolate harboring blaVIM-4 and mcr-9 was recovered from a pediatric patient in a U.S. hospital. The blaVIM-4 and mcr-9 genes are carried on the same IncH12 plasmid, pME-1a. The isolate tested susceptible to colistin, without observed induction of colistin resistan...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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American Society for Microbiology
2019-10-01
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| Series: | mSphere |
| Subjects: | |
| Online Access: | https://journals.asm.org/doi/10.1128/mSphere.00629-19 |
| _version_ | 1831522999835033600 |
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| author | Kalyan D. Chavda Lars F. Westblade Michael J. Satlin Andrew C. Hemmert Mariana Castanheira Stephen G. Jenkins Liang Chen Barry N. Kreiswirth |
| author_facet | Kalyan D. Chavda Lars F. Westblade Michael J. Satlin Andrew C. Hemmert Mariana Castanheira Stephen G. Jenkins Liang Chen Barry N. Kreiswirth |
| author_sort | Kalyan D. Chavda |
| collection | DOAJ |
| description | ABSTRACT An Enterobacter hormaechei isolate harboring blaVIM-4 and mcr-9 was recovered from a pediatric patient in a U.S. hospital. The blaVIM-4 and mcr-9 genes are carried on the same IncH12 plasmid, pME-1a. The isolate tested susceptible to colistin, without observed induction of colistin resistance. The mcr-9 gene is located between two insertion elements, IS903 and IS1, but lacks the downstream regulatory genes (qseC and qseB) found in other isolates that harbor mcr-9. IMPORTANCE We describe the complete genome assembly and sequence of a clinical Enterobacter isolate harboring both blaVIM-4 and mcr-9 recovered from a pediatric patient in the United States with a history of travel to Egypt. Moreover, to the best of our knowledge, this is the first report of an Enterobacter isolate harboring both blaVIM-4 and mcr-9 from the United States. The blaVIM-4 and mcr-9 genes are carried on the same IncH12 plasmid, pME-1a. The isolate tested susceptible to colistin, without observed induction of colistin resistance. The mcr-9 gene is located between two insertion elements, IS903 and IS1, but lacks the downstream regulatory genes (qseC and qseB) found in other isolates that harbor mcr-9. |
| first_indexed | 2024-12-14T06:57:20Z |
| format | Article |
| id | doaj.art-0b8e118714594bccadecadd8003f52c8 |
| institution | Directory Open Access Journal |
| issn | 2379-5042 |
| language | English |
| last_indexed | 2024-12-14T06:57:20Z |
| publishDate | 2019-10-01 |
| publisher | American Society for Microbiology |
| record_format | Article |
| series | mSphere |
| spelling | doaj.art-0b8e118714594bccadecadd8003f52c82022-12-21T23:12:36ZengAmerican Society for MicrobiologymSphere2379-50422019-10-014510.1128/mSphere.00629-19First Report of <italic toggle="yes">bla</italic><sub>VIM-4</sub>- and <italic toggle="yes">mcr-9</italic>-Coharboring <italic toggle="yes">Enterobacter</italic> Species Isolated from a Pediatric PatientKalyan D. Chavda0Lars F. Westblade1Michael J. Satlin2Andrew C. Hemmert3Mariana Castanheira4Stephen G. Jenkins5Liang Chen6Barry N. Kreiswirth7Center for Discovery and Innovation, Hackensack Meridian Health, Nutley, New Jersey, USADivision of Infectious Diseases, Weill Cornell Medicine, New York, New York, USADivision of Infectious Diseases, Weill Cornell Medicine, New York, New York, USABioFire Diagnostics, LLC, Salt Lake City, Utah, USAJMI Laboratories, North Liberty, Iowa, USADivision of Infectious Diseases, Weill Cornell Medicine, New York, New York, USACenter for Discovery and Innovation, Hackensack Meridian Health, Nutley, New Jersey, USACenter for Discovery and Innovation, Hackensack Meridian Health, Nutley, New Jersey, USAABSTRACT An Enterobacter hormaechei isolate harboring blaVIM-4 and mcr-9 was recovered from a pediatric patient in a U.S. hospital. The blaVIM-4 and mcr-9 genes are carried on the same IncH12 plasmid, pME-1a. The isolate tested susceptible to colistin, without observed induction of colistin resistance. The mcr-9 gene is located between two insertion elements, IS903 and IS1, but lacks the downstream regulatory genes (qseC and qseB) found in other isolates that harbor mcr-9. IMPORTANCE We describe the complete genome assembly and sequence of a clinical Enterobacter isolate harboring both blaVIM-4 and mcr-9 recovered from a pediatric patient in the United States with a history of travel to Egypt. Moreover, to the best of our knowledge, this is the first report of an Enterobacter isolate harboring both blaVIM-4 and mcr-9 from the United States. The blaVIM-4 and mcr-9 genes are carried on the same IncH12 plasmid, pME-1a. The isolate tested susceptible to colistin, without observed induction of colistin resistance. The mcr-9 gene is located between two insertion elements, IS903 and IS1, but lacks the downstream regulatory genes (qseC and qseB) found in other isolates that harbor mcr-9.https://journals.asm.org/doi/10.1128/mSphere.00629-19carbapenemcarbapenemasecolistinEnterobactermcr-9VIM |
| spellingShingle | Kalyan D. Chavda Lars F. Westblade Michael J. Satlin Andrew C. Hemmert Mariana Castanheira Stephen G. Jenkins Liang Chen Barry N. Kreiswirth First Report of <italic toggle="yes">bla</italic><sub>VIM-4</sub>- and <italic toggle="yes">mcr-9</italic>-Coharboring <italic toggle="yes">Enterobacter</italic> Species Isolated from a Pediatric Patient mSphere carbapenem carbapenemase colistin Enterobacter mcr-9 VIM |
| title | First Report of <italic toggle="yes">bla</italic><sub>VIM-4</sub>- and <italic toggle="yes">mcr-9</italic>-Coharboring <italic toggle="yes">Enterobacter</italic> Species Isolated from a Pediatric Patient |
| title_full | First Report of <italic toggle="yes">bla</italic><sub>VIM-4</sub>- and <italic toggle="yes">mcr-9</italic>-Coharboring <italic toggle="yes">Enterobacter</italic> Species Isolated from a Pediatric Patient |
| title_fullStr | First Report of <italic toggle="yes">bla</italic><sub>VIM-4</sub>- and <italic toggle="yes">mcr-9</italic>-Coharboring <italic toggle="yes">Enterobacter</italic> Species Isolated from a Pediatric Patient |
| title_full_unstemmed | First Report of <italic toggle="yes">bla</italic><sub>VIM-4</sub>- and <italic toggle="yes">mcr-9</italic>-Coharboring <italic toggle="yes">Enterobacter</italic> Species Isolated from a Pediatric Patient |
| title_short | First Report of <italic toggle="yes">bla</italic><sub>VIM-4</sub>- and <italic toggle="yes">mcr-9</italic>-Coharboring <italic toggle="yes">Enterobacter</italic> Species Isolated from a Pediatric Patient |
| title_sort | first report of italic toggle yes bla italic sub vim 4 sub and italic toggle yes mcr 9 italic coharboring italic toggle yes enterobacter italic species isolated from a pediatric patient |
| topic | carbapenem carbapenemase colistin Enterobacter mcr-9 VIM |
| url | https://journals.asm.org/doi/10.1128/mSphere.00629-19 |
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