CSF Surfactant Protein Changes in Preterm Infants After Intraventricular Hemorrhage
Introduction: Surfactant proteins (SP) have been shown to be inherent proteins of the human CNS and are altered during acute and chronic disturbances of CSF circulation. Aim of the study was to examine the changes of surfactant protein concentrations in CSF of preterm babies suffering from intravent...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2020-09-01
|
| Series: | Frontiers in Pediatrics |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fped.2020.572851/full |
| _version_ | 1818295124020428800 |
|---|---|
| author | Matthias Krause Wolfgang Härtig Wolfgang Härtig Cynthia Vanessa Mahr Cindy Richter Julia Schob Joana Puchta Joana Puchta Karl-Titus Hoffmann Ulf Nestler Ulrich Thome Matthias Knüpfer Corinna Gebauer Stefan Schob |
| author_facet | Matthias Krause Wolfgang Härtig Wolfgang Härtig Cynthia Vanessa Mahr Cindy Richter Julia Schob Joana Puchta Joana Puchta Karl-Titus Hoffmann Ulf Nestler Ulrich Thome Matthias Knüpfer Corinna Gebauer Stefan Schob |
| author_sort | Matthias Krause |
| collection | DOAJ |
| description | Introduction: Surfactant proteins (SP) have been shown to be inherent proteins of the human CNS and are altered during acute and chronic disturbances of CSF circulation. Aim of the study was to examine the changes of surfactant protein concentrations in CSF of preterm babies suffering from intraventricular hemorrhage.Patients and Methods: Consecutive CSF samples of 21 preterm infants with intraventricular hemorrhages (IVH) and posthemorrhagic hydrocephalus (PHHC) were collected at primary intervention, after 5–10 days and at time of shunt insertion ~50 days after hemorrhage. Samples were analyzed for surfactant proteins A, B, C, and G by ELISA assays and the results were compared to 35 hydrocephalus patients (HC) without hemorrhage and 6 newborn control patients.Results and Discussion: Premature patients with IVH showed a significant elevation of surfactant proteins SP-A, C, and G compared to HC and control groups: mean values for the respective groups were SP-A 4.19 vs. 1.08 vs. 0.38 ng/ml. Mean SP-C 3.63 vs. 1.47 vs. 0.48 ng/ml. Mean SP-G 3.86 vs. 0.17 vs. 0.2 ng/ml. SP-A and G concentrations were slowly falling over time without reaching normal values. SP-C levels declined faster following neurosurgical interventions and reached levels comparable to those of hydrocephalus patients without hemorrhage.Conclusion: Intraventricular hemorrhages of premature infants cause posthemorrhagic CSF flow disturbance and are associated with highly significant elevations of surfactant proteins A, C, and G independent of total CSF protein concentrations. |
| first_indexed | 2024-12-13T03:42:39Z |
| format | Article |
| id | doaj.art-0b8f401e2ec54ff6ab5087cf4264b219 |
| institution | Directory Open Access Journal |
| issn | 2296-2360 |
| language | English |
| last_indexed | 2024-12-13T03:42:39Z |
| publishDate | 2020-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pediatrics |
| spelling | doaj.art-0b8f401e2ec54ff6ab5087cf4264b2192022-12-22T00:00:54ZengFrontiers Media S.A.Frontiers in Pediatrics2296-23602020-09-01810.3389/fped.2020.572851572851CSF Surfactant Protein Changes in Preterm Infants After Intraventricular HemorrhageMatthias Krause0Wolfgang Härtig1Wolfgang Härtig2Cynthia Vanessa Mahr3Cindy Richter4Julia Schob5Joana Puchta6Joana Puchta7Karl-Titus Hoffmann8Ulf Nestler9Ulrich Thome10Matthias Knüpfer11Corinna Gebauer12Stefan Schob13Department of Neurosurgery, University Hospital Leipzig, Leipzig, GermanyPaul Flechsig Institute for Brain Research, Medical Faculty of University Leipzig, Leipzig, GermanyDepartment of Neuroradiology, University Hospital Leipzig, Leipzig, GermanyDepartment of Neurosurgery, University Hospital Leipzig, Leipzig, GermanyDepartment of Neuroradiology, University Hospital Leipzig, Leipzig, GermanyDepartment of Ophthalmology, University Hospital Leipzig, Leipzig, GermanyPaul Flechsig Institute for Brain Research, Medical Faculty of University Leipzig, Leipzig, GermanyDepartment of Neuroradiology, University Hospital Leipzig, Leipzig, GermanyDepartment of Neuroradiology, University Hospital Leipzig, Leipzig, GermanyDepartment of Neurosurgery, University Hospital Leipzig, Leipzig, GermanyDepartment of Neonatology, University Hospital Leipzig, Leipzig, GermanyDepartment of Neonatology, University Hospital Leipzig, Leipzig, GermanyDepartment of Neonatology, University Hospital Leipzig, Leipzig, GermanyDepartment of Neuroradiology, University Hospital Leipzig, Leipzig, GermanyIntroduction: Surfactant proteins (SP) have been shown to be inherent proteins of the human CNS and are altered during acute and chronic disturbances of CSF circulation. Aim of the study was to examine the changes of surfactant protein concentrations in CSF of preterm babies suffering from intraventricular hemorrhage.Patients and Methods: Consecutive CSF samples of 21 preterm infants with intraventricular hemorrhages (IVH) and posthemorrhagic hydrocephalus (PHHC) were collected at primary intervention, after 5–10 days and at time of shunt insertion ~50 days after hemorrhage. Samples were analyzed for surfactant proteins A, B, C, and G by ELISA assays and the results were compared to 35 hydrocephalus patients (HC) without hemorrhage and 6 newborn control patients.Results and Discussion: Premature patients with IVH showed a significant elevation of surfactant proteins SP-A, C, and G compared to HC and control groups: mean values for the respective groups were SP-A 4.19 vs. 1.08 vs. 0.38 ng/ml. Mean SP-C 3.63 vs. 1.47 vs. 0.48 ng/ml. Mean SP-G 3.86 vs. 0.17 vs. 0.2 ng/ml. SP-A and G concentrations were slowly falling over time without reaching normal values. SP-C levels declined faster following neurosurgical interventions and reached levels comparable to those of hydrocephalus patients without hemorrhage.Conclusion: Intraventricular hemorrhages of premature infants cause posthemorrhagic CSF flow disturbance and are associated with highly significant elevations of surfactant proteins A, C, and G independent of total CSF protein concentrations.https://www.frontiersin.org/article/10.3389/fped.2020.572851/fullintraventriclar hemorrhagehydrocephalussurfactantpretermcerebrospinal fluid |
| spellingShingle | Matthias Krause Wolfgang Härtig Wolfgang Härtig Cynthia Vanessa Mahr Cindy Richter Julia Schob Joana Puchta Joana Puchta Karl-Titus Hoffmann Ulf Nestler Ulrich Thome Matthias Knüpfer Corinna Gebauer Stefan Schob CSF Surfactant Protein Changes in Preterm Infants After Intraventricular Hemorrhage Frontiers in Pediatrics intraventriclar hemorrhage hydrocephalus surfactant preterm cerebrospinal fluid |
| title | CSF Surfactant Protein Changes in Preterm Infants After Intraventricular Hemorrhage |
| title_full | CSF Surfactant Protein Changes in Preterm Infants After Intraventricular Hemorrhage |
| title_fullStr | CSF Surfactant Protein Changes in Preterm Infants After Intraventricular Hemorrhage |
| title_full_unstemmed | CSF Surfactant Protein Changes in Preterm Infants After Intraventricular Hemorrhage |
| title_short | CSF Surfactant Protein Changes in Preterm Infants After Intraventricular Hemorrhage |
| title_sort | csf surfactant protein changes in preterm infants after intraventricular hemorrhage |
| topic | intraventriclar hemorrhage hydrocephalus surfactant preterm cerebrospinal fluid |
| url | https://www.frontiersin.org/article/10.3389/fped.2020.572851/full |
| work_keys_str_mv | AT matthiaskrause csfsurfactantproteinchangesinpreterminfantsafterintraventricularhemorrhage AT wolfganghartig csfsurfactantproteinchangesinpreterminfantsafterintraventricularhemorrhage AT wolfganghartig csfsurfactantproteinchangesinpreterminfantsafterintraventricularhemorrhage AT cynthiavanessamahr csfsurfactantproteinchangesinpreterminfantsafterintraventricularhemorrhage AT cindyrichter csfsurfactantproteinchangesinpreterminfantsafterintraventricularhemorrhage AT juliaschob csfsurfactantproteinchangesinpreterminfantsafterintraventricularhemorrhage AT joanapuchta csfsurfactantproteinchangesinpreterminfantsafterintraventricularhemorrhage AT joanapuchta csfsurfactantproteinchangesinpreterminfantsafterintraventricularhemorrhage AT karltitushoffmann csfsurfactantproteinchangesinpreterminfantsafterintraventricularhemorrhage AT ulfnestler csfsurfactantproteinchangesinpreterminfantsafterintraventricularhemorrhage AT ulrichthome csfsurfactantproteinchangesinpreterminfantsafterintraventricularhemorrhage AT matthiasknupfer csfsurfactantproteinchangesinpreterminfantsafterintraventricularhemorrhage AT corinnagebauer csfsurfactantproteinchangesinpreterminfantsafterintraventricularhemorrhage AT stefanschob csfsurfactantproteinchangesinpreterminfantsafterintraventricularhemorrhage |