Functional groups on wheat (Triticum aestivum) root surface affect aluminium transverse accumulation
Plant root growth is inhibited markedly by aluminium (Al) even at micromolar concentration and Al is mainly accumulated in plant roots outer layer cell walls. But the underlying reason for this asymmetric transverse distribution is unknown. In this study, two wheat (Triticum aestivum L.) genotypes E...
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Elsevier
2022-11-01
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| Series: | Ecotoxicology and Environmental Safety |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651322010181 |
| _version_ | 1797991034230145024 |
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| author | Zhigen Li Fan Huang Yixia Shen Sihao Ling |
| author_facet | Zhigen Li Fan Huang Yixia Shen Sihao Ling |
| author_sort | Zhigen Li |
| collection | DOAJ |
| description | Plant root growth is inhibited markedly by aluminium (Al) even at micromolar concentration and Al is mainly accumulated in plant roots outer layer cell walls. But the underlying reason for this asymmetric transverse distribution is unknown. In this study, two wheat (Triticum aestivum L.) genotypes ET8 and ES8 differing in Al resistance were investigated by hydroculture. The Al-tolerant ET8 expressed a higher root elongation rate (RER) than Al-sensitive ES8 under Al stress. Morphological examination showed symptoms such as root surface ruptures were observed in ET8 and ES8, with ES8 being more obvious. The cation exchange capacity (CEC) values of root tips of ES8 under different Al concentrations are higher than those of ET8. The sensitive genotype ES8 accumulated more Al than ET8 in plant apical root tips as well as cell walls. Under 48 h Al exposure, the root cell wall pectin concentration was increased with a higher magnitude in ES8 than in ET8. The functional groups on ET8 and ES8 roots outer layer and inner cells were investigated by Fourier transform infrared spectrometry (FTIR) under Al stress. The FTIR spectra of selected examined areas showed that the characteristic absorption peaks were located at 1692, 2920, and 3380 cm−1. The outer layer cells had stronger peaks than inner cells at wavenumber 1680–1740 cm−1, indicating root outer layer cells contain more carboxyls in both ET8 and ES8. The results demonstrate that Al transverse distribution on plants apical root cross section is likely influenced by functional groups such as negatively charged carboxylic acid. |
| first_indexed | 2024-04-11T08:46:01Z |
| format | Article |
| id | doaj.art-0b9071e402e24ed9bf054ad4c28dc9cf |
| institution | Directory Open Access Journal |
| issn | 0147-6513 |
| language | English |
| last_indexed | 2024-04-11T08:46:01Z |
| publishDate | 2022-11-01 |
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| series | Ecotoxicology and Environmental Safety |
| spelling | doaj.art-0b9071e402e24ed9bf054ad4c28dc9cf2022-12-22T04:33:58ZengElsevierEcotoxicology and Environmental Safety0147-65132022-11-01246114178Functional groups on wheat (Triticum aestivum) root surface affect aluminium transverse accumulationZhigen Li0Fan Huang1Yixia Shen2Sihao Ling3School of Life Science, Shaoxing University, Shaoxing 312000, China; College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China; Corresponding author at: School of Life Science, Shaoxing University, Shaoxing 312000, China,.Tea Research Institute of Sichuan Academy of Agricultural Science, Chengdu 610066, ChinaSchool of Life Science, Shaoxing University, Shaoxing 312000, ChinaSchool of Life Science, Shaoxing University, Shaoxing 312000, ChinaPlant root growth is inhibited markedly by aluminium (Al) even at micromolar concentration and Al is mainly accumulated in plant roots outer layer cell walls. But the underlying reason for this asymmetric transverse distribution is unknown. In this study, two wheat (Triticum aestivum L.) genotypes ET8 and ES8 differing in Al resistance were investigated by hydroculture. The Al-tolerant ET8 expressed a higher root elongation rate (RER) than Al-sensitive ES8 under Al stress. Morphological examination showed symptoms such as root surface ruptures were observed in ET8 and ES8, with ES8 being more obvious. The cation exchange capacity (CEC) values of root tips of ES8 under different Al concentrations are higher than those of ET8. The sensitive genotype ES8 accumulated more Al than ET8 in plant apical root tips as well as cell walls. Under 48 h Al exposure, the root cell wall pectin concentration was increased with a higher magnitude in ES8 than in ET8. The functional groups on ET8 and ES8 roots outer layer and inner cells were investigated by Fourier transform infrared spectrometry (FTIR) under Al stress. The FTIR spectra of selected examined areas showed that the characteristic absorption peaks were located at 1692, 2920, and 3380 cm−1. The outer layer cells had stronger peaks than inner cells at wavenumber 1680–1740 cm−1, indicating root outer layer cells contain more carboxyls in both ET8 and ES8. The results demonstrate that Al transverse distribution on plants apical root cross section is likely influenced by functional groups such as negatively charged carboxylic acid.http://www.sciencedirect.com/science/article/pii/S0147651322010181WheatPlant rootFunctional groupAluminiumTransverse distribution |
| spellingShingle | Zhigen Li Fan Huang Yixia Shen Sihao Ling Functional groups on wheat (Triticum aestivum) root surface affect aluminium transverse accumulation Ecotoxicology and Environmental Safety Wheat Plant root Functional group Aluminium Transverse distribution |
| title | Functional groups on wheat (Triticum aestivum) root surface affect aluminium transverse accumulation |
| title_full | Functional groups on wheat (Triticum aestivum) root surface affect aluminium transverse accumulation |
| title_fullStr | Functional groups on wheat (Triticum aestivum) root surface affect aluminium transverse accumulation |
| title_full_unstemmed | Functional groups on wheat (Triticum aestivum) root surface affect aluminium transverse accumulation |
| title_short | Functional groups on wheat (Triticum aestivum) root surface affect aluminium transverse accumulation |
| title_sort | functional groups on wheat triticum aestivum root surface affect aluminium transverse accumulation |
| topic | Wheat Plant root Functional group Aluminium Transverse distribution |
| url | http://www.sciencedirect.com/science/article/pii/S0147651322010181 |
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