Discordant Dose-Dependent Metabolic Effects of Eicosapentanoic Acid in Diet-Induced Obese Mice
Obesity is a widespread epidemic that increases the risk for several metabolic diseases. Despite several beneficial health effects of eicosapentaenoic acid (C20:5n-3, EPA), previous studies have used very high doses of EPA. In this study, dose-dependent effects of EPA on metabolic outcomes were dete...
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MDPI AG
2020-05-01
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| Series: | Nutrients |
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| Online Access: | https://www.mdpi.com/2072-6643/12/5/1342 |
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| author | Mandana Pahlavani Latha Ramalingam Emily K. Miller Hanna Davis Shane Scoggin Naima Moustaid-Moussa |
| author_facet | Mandana Pahlavani Latha Ramalingam Emily K. Miller Hanna Davis Shane Scoggin Naima Moustaid-Moussa |
| author_sort | Mandana Pahlavani |
| collection | DOAJ |
| description | Obesity is a widespread epidemic that increases the risk for several metabolic diseases. Despite several beneficial health effects of eicosapentaenoic acid (C20:5n-3, EPA), previous studies have used very high doses of EPA. In this study, dose-dependent effects of EPA on metabolic outcomes were determined in diet-induced obese mice. We used B6 male mice, fed high-fat diet (HF, 45% kcal fat) or HF diet supplemented with 9, 18, and 36 g/kg of EPA-enriched fish oil for 14 weeks. We conducted metabolic phenotyping during the feeding period, and harvested tissues and blood at termination. Only mice fed 36 g/kg of EPA significantly (<i>p</i> < 0.05) lowered body weight, fat content and epididymal fat pad weight, compared to HF. Both 18 and 36 g/kg doses of EPA significantly increased glucose clearance and insulin sensitivity, compared to HF or 9 g/kg of EPA. Locomotor activity was significantly increased with both 18 and 36 g/kg doses of EPA. Interestingly, all doses of EPA compared to HF, significantly increased energy expenditure and oxygen consumption and significantly reduced serum insulin, leptin, and triglycerides levels. These results demonstrate weight- and adiposity-independent metabolic benefits of EPA, at doses comparable to those currently used to treat hypertriglyceridemia. |
| first_indexed | 2024-03-10T19:58:05Z |
| format | Article |
| id | doaj.art-0b9101884752467fbfc9ba130a0704fd |
| institution | Directory Open Access Journal |
| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-10T19:58:05Z |
| publishDate | 2020-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nutrients |
| spelling | doaj.art-0b9101884752467fbfc9ba130a0704fd2023-11-19T23:47:01ZengMDPI AGNutrients2072-66432020-05-01125134210.3390/nu12051342Discordant Dose-Dependent Metabolic Effects of Eicosapentanoic Acid in Diet-Induced Obese MiceMandana Pahlavani0Latha Ramalingam1Emily K. Miller2Hanna Davis3Shane Scoggin4Naima Moustaid-Moussa5Department of Nutritional Sciences, and Obesity Research Institute, Texas Tech University, Lubbock, TX 79409, USADepartment of Nutritional Sciences, and Obesity Research Institute, Texas Tech University, Lubbock, TX 79409, USADepartment of Nutritional Sciences, and Obesity Research Institute, Texas Tech University, Lubbock, TX 79409, USADepartment of Nutritional Sciences, and Obesity Research Institute, Texas Tech University, Lubbock, TX 79409, USADepartment of Nutritional Sciences, and Obesity Research Institute, Texas Tech University, Lubbock, TX 79409, USADepartment of Nutritional Sciences, and Obesity Research Institute, Texas Tech University, Lubbock, TX 79409, USAObesity is a widespread epidemic that increases the risk for several metabolic diseases. Despite several beneficial health effects of eicosapentaenoic acid (C20:5n-3, EPA), previous studies have used very high doses of EPA. In this study, dose-dependent effects of EPA on metabolic outcomes were determined in diet-induced obese mice. We used B6 male mice, fed high-fat diet (HF, 45% kcal fat) or HF diet supplemented with 9, 18, and 36 g/kg of EPA-enriched fish oil for 14 weeks. We conducted metabolic phenotyping during the feeding period, and harvested tissues and blood at termination. Only mice fed 36 g/kg of EPA significantly (<i>p</i> < 0.05) lowered body weight, fat content and epididymal fat pad weight, compared to HF. Both 18 and 36 g/kg doses of EPA significantly increased glucose clearance and insulin sensitivity, compared to HF or 9 g/kg of EPA. Locomotor activity was significantly increased with both 18 and 36 g/kg doses of EPA. Interestingly, all doses of EPA compared to HF, significantly increased energy expenditure and oxygen consumption and significantly reduced serum insulin, leptin, and triglycerides levels. These results demonstrate weight- and adiposity-independent metabolic benefits of EPA, at doses comparable to those currently used to treat hypertriglyceridemia.https://www.mdpi.com/2072-6643/12/5/1342eicosapentaenoic aciddose-responseglucose homeostasismiceobesityomega 3 fatty acids |
| spellingShingle | Mandana Pahlavani Latha Ramalingam Emily K. Miller Hanna Davis Shane Scoggin Naima Moustaid-Moussa Discordant Dose-Dependent Metabolic Effects of Eicosapentanoic Acid in Diet-Induced Obese Mice Nutrients eicosapentaenoic acid dose-response glucose homeostasis mice obesity omega 3 fatty acids |
| title | Discordant Dose-Dependent Metabolic Effects of Eicosapentanoic Acid in Diet-Induced Obese Mice |
| title_full | Discordant Dose-Dependent Metabolic Effects of Eicosapentanoic Acid in Diet-Induced Obese Mice |
| title_fullStr | Discordant Dose-Dependent Metabolic Effects of Eicosapentanoic Acid in Diet-Induced Obese Mice |
| title_full_unstemmed | Discordant Dose-Dependent Metabolic Effects of Eicosapentanoic Acid in Diet-Induced Obese Mice |
| title_short | Discordant Dose-Dependent Metabolic Effects of Eicosapentanoic Acid in Diet-Induced Obese Mice |
| title_sort | discordant dose dependent metabolic effects of eicosapentanoic acid in diet induced obese mice |
| topic | eicosapentaenoic acid dose-response glucose homeostasis mice obesity omega 3 fatty acids |
| url | https://www.mdpi.com/2072-6643/12/5/1342 |
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