Fabrication of Sustained Release Curcumin-Loaded Solid Lipid Nanoparticles (Cur-SLNs) as a Potential Drug Delivery System for the Treatment of Lung Cancer: Optimization of Formulation and In Vitro Biological Evaluation
The goal of current research was to develop a new form of effective drug, curcumin-loaded solid lipid nanoparticles (Cur-SLNs) and test its efficacy in the treatment of lung cancer. Different batches of SLNs were prepared by the emulsification–ultrasonication method. For the optimization of formulat...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-01-01
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| Series: | Polymers |
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| Online Access: | https://www.mdpi.com/2073-4360/15/3/542 |
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| author | Mohammad Akhlaquer Rahman Abuzer Ali Mohamed Rahamathulla Shahana Salam Umme Hani Shadma Wahab Musarrat Husain Warsi Mohammad Yusuf Amena Ali Vineet Mittal Ranjit Kumar Harwansh |
| author_facet | Mohammad Akhlaquer Rahman Abuzer Ali Mohamed Rahamathulla Shahana Salam Umme Hani Shadma Wahab Musarrat Husain Warsi Mohammad Yusuf Amena Ali Vineet Mittal Ranjit Kumar Harwansh |
| author_sort | Mohammad Akhlaquer Rahman |
| collection | DOAJ |
| description | The goal of current research was to develop a new form of effective drug, curcumin-loaded solid lipid nanoparticles (Cur-SLNs) and test its efficacy in the treatment of lung cancer. Different batches of SLNs were prepared by the emulsification–ultrasonication method. For the optimization of formulation, each batch was evaluated for particle size, polydispersity index (PI), zeta potential (ZP), entrapment efficiency (EE) and drug loading (DL). The formulation components and process parameters largely affected the quality of SLNs. The SLNs obtained with particle size, 114.9 ± 1.36 nm; PI, 0.112 ± 0.005; ZP, −32.3 ± 0.30 mV; EE, 69.74 ± 2.03%, and DL, 0.81 ± 0.04% was designated as an optimized formulation. The formulation was freeze-dried to remove excess water to improve the physical stability. Freeze-dried Cur-SLNs showed 99.32% of drug release and demonstrated a burst effect trailed by sustained release up to 120 h periods. The erythrocyte toxicity study of Cur-SLNs and its components demonstrated moderate hemolytic potential towards red blood cells (RBCs). The cytotoxic potential of the formulation and plain curcumin was estimated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay against A549 cell line. After 48 h of incubation, Cur-SLNs demonstrated more cytotoxicity (IC<sub>50</sub> = 26.12 ± 1.24 µM) than plain curcumin (IC<sub>50</sub> = 35.12 ± 2.33 µM). Moreover, the cellular uptake of curcumin was found to be significantly higher from Cur-SLNs (682.08 ± 6.33 ng/µg) compared to plain curcumin (162.4 ± 4.2 ng/µg). Additionally, the optimized formulation was found to be stable over the period of 90 days of storage. Hence, curcumin-loaded SLNs can be prepared using the proposed cost effective method, and can be utilized as an effective drug delivery system for the treatment of lung cancer, provided in vivo studies warrant a similar outcome. |
| first_indexed | 2024-03-11T09:29:03Z |
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| id | doaj.art-0b940b6074484c25b39212a5bfa74333 |
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| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-11T09:29:03Z |
| publishDate | 2023-01-01 |
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| series | Polymers |
| spelling | doaj.art-0b940b6074484c25b39212a5bfa743332023-11-16T17:47:03ZengMDPI AGPolymers2073-43602023-01-0115354210.3390/polym15030542Fabrication of Sustained Release Curcumin-Loaded Solid Lipid Nanoparticles (Cur-SLNs) as a Potential Drug Delivery System for the Treatment of Lung Cancer: Optimization of Formulation and In Vitro Biological EvaluationMohammad Akhlaquer Rahman0Abuzer Ali1Mohamed Rahamathulla2Shahana Salam3Umme Hani4Shadma Wahab5Musarrat Husain Warsi6Mohammad Yusuf7Amena Ali8Vineet Mittal9Ranjit Kumar Harwansh10Department of Pharmaceutics and Industrial Pharmacy, College of Pharmacy, Taif University, P.O. Box 11099, Taif 21944, Saudi ArabiaDepartment of Pharmacognosy, College of Pharmacy, Taif University, P.O. Box 11099, Taif 21944, Saudi ArabiaDepartment of Pharmaceutics, College of Pharmacy, King Khalid University, P.O. Box 62236, Abha 62223, Saudi ArabiaDepartment of Pharmaceutical Chemistry, College of Pharmacy, Prince Sattam Bin Abdulaziz University, P.O. Box 173, Al-Kharj 11942, Saudi ArabiaDepartment of Pharmaceutics, College of Pharmacy, King Khalid University, P.O. Box 62236, Abha 62223, Saudi ArabiaDepartment of Pharmacognosy, College of Pharmacy, King Khalid University, P.O. Box 62236, Abha 62529, Saudi ArabiaDepartment of Pharmaceutics and Industrial Pharmacy, College of Pharmacy, Taif University, P.O. Box 11099, Taif 21944, Saudi ArabiaDepartment of Clinical Pharmacy, College of Pharmacy, Taif University, P.O. Box 11099, Taif 21944, Saudi ArabiaDepartment of Pharmaceutical Chemistry, College of Pharmacy, Taif University, P.O. Box 11099, Taif 21944, Saudi ArabiaDepartment of Pharmaceutical Sciences, Maharshi Dayanad University, Rohtak 124001, IndiaInstitute of Pharmaceutical Research, GLA University, Mathura 281406, IndiaThe goal of current research was to develop a new form of effective drug, curcumin-loaded solid lipid nanoparticles (Cur-SLNs) and test its efficacy in the treatment of lung cancer. Different batches of SLNs were prepared by the emulsification–ultrasonication method. For the optimization of formulation, each batch was evaluated for particle size, polydispersity index (PI), zeta potential (ZP), entrapment efficiency (EE) and drug loading (DL). The formulation components and process parameters largely affected the quality of SLNs. The SLNs obtained with particle size, 114.9 ± 1.36 nm; PI, 0.112 ± 0.005; ZP, −32.3 ± 0.30 mV; EE, 69.74 ± 2.03%, and DL, 0.81 ± 0.04% was designated as an optimized formulation. The formulation was freeze-dried to remove excess water to improve the physical stability. Freeze-dried Cur-SLNs showed 99.32% of drug release and demonstrated a burst effect trailed by sustained release up to 120 h periods. The erythrocyte toxicity study of Cur-SLNs and its components demonstrated moderate hemolytic potential towards red blood cells (RBCs). The cytotoxic potential of the formulation and plain curcumin was estimated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay against A549 cell line. After 48 h of incubation, Cur-SLNs demonstrated more cytotoxicity (IC<sub>50</sub> = 26.12 ± 1.24 µM) than plain curcumin (IC<sub>50</sub> = 35.12 ± 2.33 µM). Moreover, the cellular uptake of curcumin was found to be significantly higher from Cur-SLNs (682.08 ± 6.33 ng/µg) compared to plain curcumin (162.4 ± 4.2 ng/µg). Additionally, the optimized formulation was found to be stable over the period of 90 days of storage. Hence, curcumin-loaded SLNs can be prepared using the proposed cost effective method, and can be utilized as an effective drug delivery system for the treatment of lung cancer, provided in vivo studies warrant a similar outcome.https://www.mdpi.com/2073-4360/15/3/542curcuminsolubilitylung cancersolid lipid nanoparticleerythrocyte toxicitycytotoxicity |
| spellingShingle | Mohammad Akhlaquer Rahman Abuzer Ali Mohamed Rahamathulla Shahana Salam Umme Hani Shadma Wahab Musarrat Husain Warsi Mohammad Yusuf Amena Ali Vineet Mittal Ranjit Kumar Harwansh Fabrication of Sustained Release Curcumin-Loaded Solid Lipid Nanoparticles (Cur-SLNs) as a Potential Drug Delivery System for the Treatment of Lung Cancer: Optimization of Formulation and In Vitro Biological Evaluation Polymers curcumin solubility lung cancer solid lipid nanoparticle erythrocyte toxicity cytotoxicity |
| title | Fabrication of Sustained Release Curcumin-Loaded Solid Lipid Nanoparticles (Cur-SLNs) as a Potential Drug Delivery System for the Treatment of Lung Cancer: Optimization of Formulation and In Vitro Biological Evaluation |
| title_full | Fabrication of Sustained Release Curcumin-Loaded Solid Lipid Nanoparticles (Cur-SLNs) as a Potential Drug Delivery System for the Treatment of Lung Cancer: Optimization of Formulation and In Vitro Biological Evaluation |
| title_fullStr | Fabrication of Sustained Release Curcumin-Loaded Solid Lipid Nanoparticles (Cur-SLNs) as a Potential Drug Delivery System for the Treatment of Lung Cancer: Optimization of Formulation and In Vitro Biological Evaluation |
| title_full_unstemmed | Fabrication of Sustained Release Curcumin-Loaded Solid Lipid Nanoparticles (Cur-SLNs) as a Potential Drug Delivery System for the Treatment of Lung Cancer: Optimization of Formulation and In Vitro Biological Evaluation |
| title_short | Fabrication of Sustained Release Curcumin-Loaded Solid Lipid Nanoparticles (Cur-SLNs) as a Potential Drug Delivery System for the Treatment of Lung Cancer: Optimization of Formulation and In Vitro Biological Evaluation |
| title_sort | fabrication of sustained release curcumin loaded solid lipid nanoparticles cur slns as a potential drug delivery system for the treatment of lung cancer optimization of formulation and in vitro biological evaluation |
| topic | curcumin solubility lung cancer solid lipid nanoparticle erythrocyte toxicity cytotoxicity |
| url | https://www.mdpi.com/2073-4360/15/3/542 |
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