New Phenotype and Mineralization of Biogenic Iron Oxide in Magnetotactic Bacteria
Many magnetotactic bacteria (MTB) biomineralize magnetite crystals that nucleate and grow inside intracellular membranous vesicles originating from invaginations of the cytoplasmic membrane. The crystals together with their surrounding membranes are referred to as magnetosomes. Magnetosome magnetite...
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| Format: | Article |
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MDPI AG
2021-11-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/11/12/3189 |
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| author | Walid Baaziz Corneliu Ghica Jefferson Cypriano Fernanda Abreu Karine Anselme Ovidiu Ersen Marcos Farina Jacques Werckmann |
| author_facet | Walid Baaziz Corneliu Ghica Jefferson Cypriano Fernanda Abreu Karine Anselme Ovidiu Ersen Marcos Farina Jacques Werckmann |
| author_sort | Walid Baaziz |
| collection | DOAJ |
| description | Many magnetotactic bacteria (MTB) biomineralize magnetite crystals that nucleate and grow inside intracellular membranous vesicles originating from invaginations of the cytoplasmic membrane. The crystals together with their surrounding membranes are referred to as magnetosomes. Magnetosome magnetite crystals nucleate and grow using iron transported inside the vesicle by specific proteins. Here, we tackle the question of the organization of magnetosomes, which are always described as constituted by linear chains of nanocrystals. In addition, it is commonly accepted that the iron oxide nanocrystals are in the magnetite-based phase. We show, in the case of a wild species of coccus-type bacterium, that there is a double organization of the magnetosomes, relatively perpendicular to each other, and that the nanocrystals are in fact maghemite. These findings were obtained, respectively, by using electron tomography of whole mounts of cells directly from the environment and high-resolution transmission electron microscopy and diffraction. Structure simulations were performed with the MacTempas software. This study opens new perspectives on the diversity of phenotypes within MTBs and allows to envisage other mechanisms of nucleation and formation of biogenic iron oxide crystals. |
| first_indexed | 2024-03-10T03:26:58Z |
| format | Article |
| id | doaj.art-0b95ac6b99584f5e9eba10cc0927dc50 |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-10T03:26:58Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-0b95ac6b99584f5e9eba10cc0927dc502023-11-23T09:49:06ZengMDPI AGNanomaterials2079-49912021-11-011112318910.3390/nano11123189New Phenotype and Mineralization of Biogenic Iron Oxide in Magnetotactic BacteriaWalid Baaziz0Corneliu Ghica1Jefferson Cypriano2Fernanda Abreu3Karine Anselme4Ovidiu Ersen5Marcos Farina6Jacques Werckmann7Institut de Physique et Chimie des Matériaux de Strasbourg (IPCMS), University of Strasbourg, 23 rue du Loess BP 43, CEDEX 2, 67034 Strasbourg, FranceNational Institute of Materials Physics, Atomistilor 405A, 077125 Magurele, RomaniaInstituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Avenida Carlos Chagas Filho, 373, CCS, UFRJ, Rio de Janeiro 21941-902, BrazilInstituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Avenida Carlos Chagas Filho, 373, CCS, UFRJ, Rio de Janeiro 21941-902, BrazilInstitut de Science des Matériaux de Mulhouse, University of Haute Alsace, 68057 Mulhouse, FranceInstitut de Physique et Chimie des Matériaux de Strasbourg (IPCMS), University of Strasbourg, 23 rue du Loess BP 43, CEDEX 2, 67034 Strasbourg, FranceInstituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-902, BrazilInstituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-902, BrazilMany magnetotactic bacteria (MTB) biomineralize magnetite crystals that nucleate and grow inside intracellular membranous vesicles originating from invaginations of the cytoplasmic membrane. The crystals together with their surrounding membranes are referred to as magnetosomes. Magnetosome magnetite crystals nucleate and grow using iron transported inside the vesicle by specific proteins. Here, we tackle the question of the organization of magnetosomes, which are always described as constituted by linear chains of nanocrystals. In addition, it is commonly accepted that the iron oxide nanocrystals are in the magnetite-based phase. We show, in the case of a wild species of coccus-type bacterium, that there is a double organization of the magnetosomes, relatively perpendicular to each other, and that the nanocrystals are in fact maghemite. These findings were obtained, respectively, by using electron tomography of whole mounts of cells directly from the environment and high-resolution transmission electron microscopy and diffraction. Structure simulations were performed with the MacTempas software. This study opens new perspectives on the diversity of phenotypes within MTBs and allows to envisage other mechanisms of nucleation and formation of biogenic iron oxide crystals.https://www.mdpi.com/2079-4991/11/12/3189magnetotactic bacteriaphenotypemagnetitemaghemiteelectron microscopyEDS |
| spellingShingle | Walid Baaziz Corneliu Ghica Jefferson Cypriano Fernanda Abreu Karine Anselme Ovidiu Ersen Marcos Farina Jacques Werckmann New Phenotype and Mineralization of Biogenic Iron Oxide in Magnetotactic Bacteria Nanomaterials magnetotactic bacteria phenotype magnetite maghemite electron microscopy EDS |
| title | New Phenotype and Mineralization of Biogenic Iron Oxide in Magnetotactic Bacteria |
| title_full | New Phenotype and Mineralization of Biogenic Iron Oxide in Magnetotactic Bacteria |
| title_fullStr | New Phenotype and Mineralization of Biogenic Iron Oxide in Magnetotactic Bacteria |
| title_full_unstemmed | New Phenotype and Mineralization of Biogenic Iron Oxide in Magnetotactic Bacteria |
| title_short | New Phenotype and Mineralization of Biogenic Iron Oxide in Magnetotactic Bacteria |
| title_sort | new phenotype and mineralization of biogenic iron oxide in magnetotactic bacteria |
| topic | magnetotactic bacteria phenotype magnetite maghemite electron microscopy EDS |
| url | https://www.mdpi.com/2079-4991/11/12/3189 |
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