Direct Light-up of cAMP Derivatives in Living Cells by Click Reactions
8-Azidoadenosine 3′,5′-cyclic monophosphate (8-azido cAMP) was directly detected in living cells, by applying Cu-free azide-alkyne cycloaddition to probe cAMP derivatives by fluorescence light-up. Fluorescence emission was generated by two non-fluorescent molecules, 8-azido cAMP as a model target an...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2013-10-01
|
| Series: | Molecules |
| Subjects: | |
| Online Access: | http://www.mdpi.com/1420-3049/18/10/12909 |
| _version_ | 1811266503731838976 |
|---|---|
| author | Yan Xu Hongshan Liu Tetsuya Hayashi Makoto Komiyama Kenichiro Ito |
| author_facet | Yan Xu Hongshan Liu Tetsuya Hayashi Makoto Komiyama Kenichiro Ito |
| author_sort | Yan Xu |
| collection | DOAJ |
| description | 8-Azidoadenosine 3′,5′-cyclic monophosphate (8-azido cAMP) was directly detected in living cells, by applying Cu-free azide-alkyne cycloaddition to probe cAMP derivatives by fluorescence light-up. Fluorescence emission was generated by two non-fluorescent molecules, 8-azido cAMP as a model target and difluorinated cyclooctyne (DIFO) reagent as a probe. The azide-alkyne cycloaddition reaction between 8-azido cAMP and DIFO induces fluorescence in 8-azido cAMP. The fluorescence emission serves as a way to probe 8-azido cAMP in cells. |
| first_indexed | 2024-04-12T20:43:20Z |
| format | Article |
| id | doaj.art-0b98827ef4b444fdb3eb82284b3eaf94 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-04-12T20:43:20Z |
| publishDate | 2013-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-0b98827ef4b444fdb3eb82284b3eaf942022-12-22T03:17:20ZengMDPI AGMolecules1420-30492013-10-011810129091291510.3390/molecules181012909Direct Light-up of cAMP Derivatives in Living Cells by Click ReactionsYan XuHongshan LiuTetsuya HayashiMakoto KomiyamaKenichiro Ito8-Azidoadenosine 3′,5′-cyclic monophosphate (8-azido cAMP) was directly detected in living cells, by applying Cu-free azide-alkyne cycloaddition to probe cAMP derivatives by fluorescence light-up. Fluorescence emission was generated by two non-fluorescent molecules, 8-azido cAMP as a model target and difluorinated cyclooctyne (DIFO) reagent as a probe. The azide-alkyne cycloaddition reaction between 8-azido cAMP and DIFO induces fluorescence in 8-azido cAMP. The fluorescence emission serves as a way to probe 8-azido cAMP in cells.http://www.mdpi.com/1420-3049/18/10/12909click chemistrycAMPfluorescencecAMP localization in living cells |
| spellingShingle | Yan Xu Hongshan Liu Tetsuya Hayashi Makoto Komiyama Kenichiro Ito Direct Light-up of cAMP Derivatives in Living Cells by Click Reactions Molecules click chemistry cAMP fluorescence cAMP localization in living cells |
| title | Direct Light-up of cAMP Derivatives in Living Cells by Click Reactions |
| title_full | Direct Light-up of cAMP Derivatives in Living Cells by Click Reactions |
| title_fullStr | Direct Light-up of cAMP Derivatives in Living Cells by Click Reactions |
| title_full_unstemmed | Direct Light-up of cAMP Derivatives in Living Cells by Click Reactions |
| title_short | Direct Light-up of cAMP Derivatives in Living Cells by Click Reactions |
| title_sort | direct light up of camp derivatives in living cells by click reactions |
| topic | click chemistry cAMP fluorescence cAMP localization in living cells |
| url | http://www.mdpi.com/1420-3049/18/10/12909 |
| work_keys_str_mv | AT yanxu directlightupofcampderivativesinlivingcellsbyclickreactions AT hongshanliu directlightupofcampderivativesinlivingcellsbyclickreactions AT tetsuyahayashi directlightupofcampderivativesinlivingcellsbyclickreactions AT makotokomiyama directlightupofcampderivativesinlivingcellsbyclickreactions AT kenichiroito directlightupofcampderivativesinlivingcellsbyclickreactions |