Adipogenic Differentiation of Muscle Derived Cells is Repressed by Inhibition of GSK-3 Activity
Intramuscular fat is important in large animal livestock species in regard to meat quality and in humans is of clinical significance in particular in relation to insulin resistance. The canonical Wnt signalling pathway has been implicated at a whole body level in regulating relative levels of adipos...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2018-06-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fvets.2018.00110/full |
| _version_ | 1818056823993794560 |
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| author | Zoe Redshaw Zoe Redshaw Paul Thomas Loughna |
| author_facet | Zoe Redshaw Zoe Redshaw Paul Thomas Loughna |
| author_sort | Zoe Redshaw |
| collection | DOAJ |
| description | Intramuscular fat is important in large animal livestock species in regard to meat quality and in humans is of clinical significance in particular in relation to insulin resistance. The canonical Wnt signalling pathway has been implicated at a whole body level in regulating relative levels of adiposity versus lean body mass. Previously we have shown that pig muscle cells can undergo adipogenic differentiation to a degree that is dependent upon the specific muscle source. In this work we examine the role of the canonical Wnt pathway which acts through inactivation of glycogen synthase kinase-3 (GSK-3) in the regulation of adipogenic differentiation in muscle cells derived from the pig semimembranosus muscle.The application of lithium chloride to muscle derived cells significantly increased the phosphorylation of GSK-3β and thus inhibited its activity thus mimicking Wnt signaling. This was associated with a significant decrease in the expression of the adipogenic transcription factor PPARγ and an almost complete inhibition of adipogenesis in the cells. The data also suggest that GSK-3α plays, at most, a small role in this process.Studies in vivo have suggested that the Wnt pathway is a major regulator of whole body adiposity. In this study we have shown that the ability of cells derived from porcine skeletal muscle to differentiate along an adipogenic lineage, in vitro, is severely impaired by mimicking the action of this pathway. This was done by inactivation of GSK-3β by the use of Lithium Chloride. |
| first_indexed | 2024-12-10T12:34:58Z |
| format | Article |
| id | doaj.art-0b993f0be58841bca141bb3194316504 |
| institution | Directory Open Access Journal |
| issn | 2297-1769 |
| language | English |
| last_indexed | 2024-12-10T12:34:58Z |
| publishDate | 2018-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj.art-0b993f0be58841bca141bb31943165042022-12-22T01:48:40ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692018-06-01510.3389/fvets.2018.00110267297Adipogenic Differentiation of Muscle Derived Cells is Repressed by Inhibition of GSK-3 ActivityZoe RedshawZoe RedshawPaul Thomas LoughnaIntramuscular fat is important in large animal livestock species in regard to meat quality and in humans is of clinical significance in particular in relation to insulin resistance. The canonical Wnt signalling pathway has been implicated at a whole body level in regulating relative levels of adiposity versus lean body mass. Previously we have shown that pig muscle cells can undergo adipogenic differentiation to a degree that is dependent upon the specific muscle source. In this work we examine the role of the canonical Wnt pathway which acts through inactivation of glycogen synthase kinase-3 (GSK-3) in the regulation of adipogenic differentiation in muscle cells derived from the pig semimembranosus muscle.The application of lithium chloride to muscle derived cells significantly increased the phosphorylation of GSK-3β and thus inhibited its activity thus mimicking Wnt signaling. This was associated with a significant decrease in the expression of the adipogenic transcription factor PPARγ and an almost complete inhibition of adipogenesis in the cells. The data also suggest that GSK-3α plays, at most, a small role in this process.Studies in vivo have suggested that the Wnt pathway is a major regulator of whole body adiposity. In this study we have shown that the ability of cells derived from porcine skeletal muscle to differentiate along an adipogenic lineage, in vitro, is severely impaired by mimicking the action of this pathway. This was done by inactivation of GSK-3β by the use of Lithium Chloride.https://www.frontiersin.org/article/10.3389/fvets.2018.00110/fullWntskeletal muscleGSK-3βPPARγadipogenesis |
| spellingShingle | Zoe Redshaw Zoe Redshaw Paul Thomas Loughna Adipogenic Differentiation of Muscle Derived Cells is Repressed by Inhibition of GSK-3 Activity Frontiers in Veterinary Science Wnt skeletal muscle GSK-3β PPARγ adipogenesis |
| title | Adipogenic Differentiation of Muscle Derived Cells is Repressed by Inhibition of GSK-3 Activity |
| title_full | Adipogenic Differentiation of Muscle Derived Cells is Repressed by Inhibition of GSK-3 Activity |
| title_fullStr | Adipogenic Differentiation of Muscle Derived Cells is Repressed by Inhibition of GSK-3 Activity |
| title_full_unstemmed | Adipogenic Differentiation of Muscle Derived Cells is Repressed by Inhibition of GSK-3 Activity |
| title_short | Adipogenic Differentiation of Muscle Derived Cells is Repressed by Inhibition of GSK-3 Activity |
| title_sort | adipogenic differentiation of muscle derived cells is repressed by inhibition of gsk 3 activity |
| topic | Wnt skeletal muscle GSK-3β PPARγ adipogenesis |
| url | https://www.frontiersin.org/article/10.3389/fvets.2018.00110/full |
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