Isolation and characterization of Bifidobacteria from broiler crops and the possibility to use as probiotics

Abstract<br /> The aim of this study is to isolate Bifidobacteria from broiler crops to obtain bacteria having the ability of adhesion to digestive canal and good activity; The isolated bacteria were passaged three serial passages in chicks for 6 days interval, after that, Three isolates were...

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Main Authors: Ammar AbdulRazzaq Tawfeeq Alani, Zeyad Tariq Mohammad Aldhanke
Format: Article
Language:Arabic
Published: College of Education for Pure Sciences 2013-06-01
Series:مجلة التربية والعلم
Subjects:
Online Access:https://edusj.mosuljournals.com/article_89787_87fa2d115f2d22f66c4e7adacb2da97b.pdf
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author Ammar AbdulRazzaq Tawfeeq Alani
Zeyad Tariq Mohammad Aldhanke
author_facet Ammar AbdulRazzaq Tawfeeq Alani
Zeyad Tariq Mohammad Aldhanke
author_sort Ammar AbdulRazzaq Tawfeeq Alani
collection DOAJ
description Abstract<br /> The aim of this study is to isolate Bifidobacteria from broiler crops to obtain bacteria having the ability of adhesion to digestive canal and good activity; The isolated bacteria were passaged three serial passages in chicks for 6 days interval, after that, Three isolates were selected and testing for probiotic and biochemical properties on them (api 20 A test one of biochemical tests which was conducted on one isolate only). These isolates were showed same properties, they were unable to grow in aerobic conditions, they couldn't produce catalase enzyme and CO2, they could produce acid, and analyze starch. They had high ability for adhesion on crop epithelial cells, and they were unable to grow in acid medium (pH<4.5) but they were able to growth in medium (pH=6). On other hand, the isolates were sensitive for most antibiotics. By using the api 20 A test, one of these isolates showed similar characters to Bifidobacterium adolescentis and possibly could be used as probiotic for broiler.
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spelling doaj.art-0b9c101c6f4448cc83073b39bafab8862022-12-22T03:16:05ZaraCollege of Education for Pure Sciencesمجلة التربية والعلم1812-125X2664-25302013-06-0126214615710.33899/edusj.2013.8978789787Isolation and characterization of Bifidobacteria from broiler crops and the possibility to use as probioticsAmmar AbdulRazzaq Tawfeeq AlaniZeyad Tariq Mohammad AldhankeAbstract<br /> The aim of this study is to isolate Bifidobacteria from broiler crops to obtain bacteria having the ability of adhesion to digestive canal and good activity; The isolated bacteria were passaged three serial passages in chicks for 6 days interval, after that, Three isolates were selected and testing for probiotic and biochemical properties on them (api 20 A test one of biochemical tests which was conducted on one isolate only). These isolates were showed same properties, they were unable to grow in aerobic conditions, they couldn't produce catalase enzyme and CO2, they could produce acid, and analyze starch. They had high ability for adhesion on crop epithelial cells, and they were unable to grow in acid medium (pH<4.5) but they were able to growth in medium (pH=6). On other hand, the isolates were sensitive for most antibiotics. By using the api 20 A test, one of these isolates showed similar characters to Bifidobacterium adolescentis and possibly could be used as probiotic for broiler.https://edusj.mosuljournals.com/article_89787_87fa2d115f2d22f66c4e7adacb2da97b.pdfbifidobacteriabroiler cropsprobioticscharacterization
spellingShingle Ammar AbdulRazzaq Tawfeeq Alani
Zeyad Tariq Mohammad Aldhanke
Isolation and characterization of Bifidobacteria from broiler crops and the possibility to use as probiotics
مجلة التربية والعلم
bifidobacteria
broiler crops
probiotics
characterization
title Isolation and characterization of Bifidobacteria from broiler crops and the possibility to use as probiotics
title_full Isolation and characterization of Bifidobacteria from broiler crops and the possibility to use as probiotics
title_fullStr Isolation and characterization of Bifidobacteria from broiler crops and the possibility to use as probiotics
title_full_unstemmed Isolation and characterization of Bifidobacteria from broiler crops and the possibility to use as probiotics
title_short Isolation and characterization of Bifidobacteria from broiler crops and the possibility to use as probiotics
title_sort isolation and characterization of bifidobacteria from broiler crops and the possibility to use as probiotics
topic bifidobacteria
broiler crops
probiotics
characterization
url https://edusj.mosuljournals.com/article_89787_87fa2d115f2d22f66c4e7adacb2da97b.pdf
work_keys_str_mv AT ammarabdulrazzaqtawfeeqalani isolationandcharacterizationofbifidobacteriafrombroilercropsandthepossibilitytouseasprobiotics
AT zeyadtariqmohammadaldhanke isolationandcharacterizationofbifidobacteriafrombroilercropsandthepossibilitytouseasprobiotics