Urine Epidermal Growth Factor, Monocyte Chemoattractant Protein-1 or Their Ratio as Biomarkers for Interstitial Fibrosis and Tubular Atrophy in Primary Glomerulonephritis
Background/Aims: The degree of tubular atrophy and interstitial fibrosis (IFTA) is an important prognostic factor in glomerulonephritis. Imbalance between pro-inflammatory cytokines such as monocyte chemoattractant protein- 1 (MCP-1) and protective cytokines such as epidermal growth factor (EGF) lik...
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| Format: | Article |
| Language: | English |
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Karger Publishers
2016-12-01
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| Series: | Kidney & Blood Pressure Research |
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| Online Access: | http://www.karger.com/Article/FullText/452595 |
| _version_ | 1818335005842079744 |
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| author | Supanat Worawichawong Suchin Worawichawong Piyanuch Radinahamed Dittapol Muntham Nuankanya Sathirapongsasuti Arkom Nongnuch Montira Assanatham Chagriya Kitiyakara |
| author_facet | Supanat Worawichawong Suchin Worawichawong Piyanuch Radinahamed Dittapol Muntham Nuankanya Sathirapongsasuti Arkom Nongnuch Montira Assanatham Chagriya Kitiyakara |
| author_sort | Supanat Worawichawong |
| collection | DOAJ |
| description | Background/Aims: The degree of tubular atrophy and interstitial fibrosis (IFTA) is an important prognostic factor in glomerulonephritis. Imbalance between pro-inflammatory cytokines such as monocyte chemoattractant protein- 1 (MCP-1) and protective cytokines such as epidermal growth factor (EGF) likely determine IFTA severity. In separate studies, elevated MCP-1 and decreased EGF have been shown to be associated with IFTA severity. In this study, we aim to evaluate the predictive value of urinary EGF/MCP-1 ratio compared to each biomarker individually for moderate to severe IFTA in primary glomerulonephritis (GN). Methods: Urine samples were collected at biopsy from primary GN (IgA nephropathy, focal and segmental glomerulosclerosis, minimal change disease, membranous nephropathy). MCP-1 and EGF were analyzed by enzyme-linked immunosorbent assay. Results: EGF, MCP-1 and EGF/MCP-1 ratio from primary GN, all correlated with IFTA (n=58). By univariate analysis, glomerular filtration rate, EGF, and EGF/MCP-1 ratio were associated with IFTA. By multivariate analysis, only EGF/MCP-1 ratio was independently associated with IFTA. EGF/MCP-1 ratio had a sensitivity of 88% and specificity of 74 % for IFTA. EGF/MCP-1 had good discrimination for IFTA (AUC=0.85), but the improvement over EGF alone was not significant. Conclusion: EGF/MCP-1 ratio is independently associated IFTA severity in primary glomerulonephritis, but the ability of EGF/MCP-1 ratio to discriminate moderate to severe IFTA may not be much better than EGF alone. |
| first_indexed | 2024-12-13T14:16:33Z |
| format | Article |
| id | doaj.art-0b9cac20a20143c7a7920950585cff60 |
| institution | Directory Open Access Journal |
| issn | 1420-4096 1423-0143 |
| language | English |
| last_indexed | 2024-12-13T14:16:33Z |
| publishDate | 2016-12-01 |
| publisher | Karger Publishers |
| record_format | Article |
| series | Kidney & Blood Pressure Research |
| spelling | doaj.art-0b9cac20a20143c7a7920950585cff602022-12-21T23:42:17ZengKarger PublishersKidney & Blood Pressure Research1420-40961423-01432016-12-01416997100710.1159/000452595452595Urine Epidermal Growth Factor, Monocyte Chemoattractant Protein-1 or Their Ratio as Biomarkers for Interstitial Fibrosis and Tubular Atrophy in Primary GlomerulonephritisSupanat WorawichawongSuchin WorawichawongPiyanuch RadinahamedDittapol MunthamNuankanya SathirapongsasutiArkom NongnuchMontira AssanathamChagriya KitiyakaraBackground/Aims: The degree of tubular atrophy and interstitial fibrosis (IFTA) is an important prognostic factor in glomerulonephritis. Imbalance between pro-inflammatory cytokines such as monocyte chemoattractant protein- 1 (MCP-1) and protective cytokines such as epidermal growth factor (EGF) likely determine IFTA severity. In separate studies, elevated MCP-1 and decreased EGF have been shown to be associated with IFTA severity. In this study, we aim to evaluate the predictive value of urinary EGF/MCP-1 ratio compared to each biomarker individually for moderate to severe IFTA in primary glomerulonephritis (GN). Methods: Urine samples were collected at biopsy from primary GN (IgA nephropathy, focal and segmental glomerulosclerosis, minimal change disease, membranous nephropathy). MCP-1 and EGF were analyzed by enzyme-linked immunosorbent assay. Results: EGF, MCP-1 and EGF/MCP-1 ratio from primary GN, all correlated with IFTA (n=58). By univariate analysis, glomerular filtration rate, EGF, and EGF/MCP-1 ratio were associated with IFTA. By multivariate analysis, only EGF/MCP-1 ratio was independently associated with IFTA. EGF/MCP-1 ratio had a sensitivity of 88% and specificity of 74 % for IFTA. EGF/MCP-1 had good discrimination for IFTA (AUC=0.85), but the improvement over EGF alone was not significant. Conclusion: EGF/MCP-1 ratio is independently associated IFTA severity in primary glomerulonephritis, but the ability of EGF/MCP-1 ratio to discriminate moderate to severe IFTA may not be much better than EGF alone.http://www.karger.com/Article/FullText/452595BiomarkerChronic kidney diseaseEGFEpidermal Growth FactorFibrosisGlomerulonephritisKidneyNGALNeutrophil gelatinase-associated lipocalinMCP-1Monocyte chemoattractant protein-1Tubulointerstitial |
| spellingShingle | Supanat Worawichawong Suchin Worawichawong Piyanuch Radinahamed Dittapol Muntham Nuankanya Sathirapongsasuti Arkom Nongnuch Montira Assanatham Chagriya Kitiyakara Urine Epidermal Growth Factor, Monocyte Chemoattractant Protein-1 or Their Ratio as Biomarkers for Interstitial Fibrosis and Tubular Atrophy in Primary Glomerulonephritis Kidney & Blood Pressure Research Biomarker Chronic kidney disease EGF Epidermal Growth Factor Fibrosis Glomerulonephritis Kidney NGAL Neutrophil gelatinase-associated lipocalin MCP-1 Monocyte chemoattractant protein-1 Tubulointerstitial |
| title | Urine Epidermal Growth Factor, Monocyte Chemoattractant Protein-1 or Their Ratio as Biomarkers for Interstitial Fibrosis and Tubular Atrophy in Primary Glomerulonephritis |
| title_full | Urine Epidermal Growth Factor, Monocyte Chemoattractant Protein-1 or Their Ratio as Biomarkers for Interstitial Fibrosis and Tubular Atrophy in Primary Glomerulonephritis |
| title_fullStr | Urine Epidermal Growth Factor, Monocyte Chemoattractant Protein-1 or Their Ratio as Biomarkers for Interstitial Fibrosis and Tubular Atrophy in Primary Glomerulonephritis |
| title_full_unstemmed | Urine Epidermal Growth Factor, Monocyte Chemoattractant Protein-1 or Their Ratio as Biomarkers for Interstitial Fibrosis and Tubular Atrophy in Primary Glomerulonephritis |
| title_short | Urine Epidermal Growth Factor, Monocyte Chemoattractant Protein-1 or Their Ratio as Biomarkers for Interstitial Fibrosis and Tubular Atrophy in Primary Glomerulonephritis |
| title_sort | urine epidermal growth factor monocyte chemoattractant protein 1 or their ratio as biomarkers for interstitial fibrosis and tubular atrophy in primary glomerulonephritis |
| topic | Biomarker Chronic kidney disease EGF Epidermal Growth Factor Fibrosis Glomerulonephritis Kidney NGAL Neutrophil gelatinase-associated lipocalin MCP-1 Monocyte chemoattractant protein-1 Tubulointerstitial |
| url | http://www.karger.com/Article/FullText/452595 |
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