Degradation of fructans and production of propionic acid by Bacteroides thetaiotaomicron are enhanced by shortage of amino acids
Bacteroides thetaiotaomicron is commonly found in the human colon and stabilizes its ecosystem by the catabolism of various polysaccharides. A model of cross-talk between the metabolism of amino acids and fructans in B. thetaiotaomicron was proposed. The growth of B. thetaiotaomicron DSM 2079 in two...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2014-12-01
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| Series: | Frontiers in Nutrition |
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| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fnut.2014.00021/full |
| _version_ | 1818110751381913600 |
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| author | Signe eAdamberg Katrin eTomson Heiki eVija Marju ePuurand Natalja eKabanova Triinu eVisnapuu Eerik eJõgi Tiina eAlamäe Kaarel eAdamberg Kaarel eAdamberg Kaarel eAdamberg |
| author_facet | Signe eAdamberg Katrin eTomson Heiki eVija Marju ePuurand Natalja eKabanova Triinu eVisnapuu Eerik eJõgi Tiina eAlamäe Kaarel eAdamberg Kaarel eAdamberg Kaarel eAdamberg |
| author_sort | Signe eAdamberg |
| collection | DOAJ |
| description | Bacteroides thetaiotaomicron is commonly found in the human colon and stabilizes its ecosystem by the catabolism of various polysaccharides. A model of cross-talk between the metabolism of amino acids and fructans in B. thetaiotaomicron was proposed. The growth of B. thetaiotaomicron DSM 2079 in two defined media containing mineral salts and vitamins, and supplemented with either 20 or 2 amino acids, was studied in an isothermal microcalorimeter. The polyfructans inulin (from chicory) and levan (synthesized using levansucrase from Pseudomonas syringae), two fructooligosaccharide preparations with different composition, sucrose and fructose were tested as substrates. The calorimetric power-time curves were substrate specific and typically multiauxic. A surplus of amino acids reduced the consumption of longer oligosaccharides (DP > 3). Bacterial growth was not detected either in the carbohydrate free medium containing amino acids or in the medium with inulin as a sole carbohydrate. In amino acid-restricted medium, fermentation leading to acetic acid formation was dominant at the beginning of growth (up to 24 h), followed by increased lactic acid production, and mainly propionic and succinic acids were produced at the end of fermentation. In the medium supplemented with 20 amino acids, the highest production of D-lactate (82 ± 33 mmol/gDW) occurred in parallel with extensive consumption (up to 17 mmol/gDW) of amino acids, especially Ser, Thr and Asp. The production of Ala and Glu was observed at growth on all substrates, and the production was enhanced under amino acid deficiency. The study revealed the influence of amino acids on fructan metabolism in B. thetaiotaomicron and showed that defined growth media are invaluable in elucidating quantitative metabolic profiles of the bacteria. Levan was shown to act as an easily degradable substrate for B. thetaiotaomicron. The effect of levan on balancing or modifying colon microbiota will be studied in further experiments. |
| first_indexed | 2024-12-11T02:52:07Z |
| format | Article |
| id | doaj.art-0ba12baa1a094c0ea85f1189db91302e |
| institution | Directory Open Access Journal |
| issn | 2296-861X |
| language | English |
| last_indexed | 2024-12-11T02:52:07Z |
| publishDate | 2014-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Nutrition |
| spelling | doaj.art-0ba12baa1a094c0ea85f1189db91302e2022-12-22T01:23:16ZengFrontiers Media S.A.Frontiers in Nutrition2296-861X2014-12-01110.3389/fnut.2014.00021119326Degradation of fructans and production of propionic acid by Bacteroides thetaiotaomicron are enhanced by shortage of amino acidsSigne eAdamberg0Katrin eTomson1Heiki eVija2Marju ePuurand3Natalja eKabanova4Triinu eVisnapuu5Eerik eJõgi6Tiina eAlamäe7Kaarel eAdamberg8Kaarel eAdamberg9Kaarel eAdamberg10Tallinn University of TechnologyCompetence Center of Food and Fermentation TechnologiesNational Institute of Chemical Physics and BiophysicsTallinn University of TechnologyCompetence Center of Food and Fermentation TechnologiesUniversity of TartuUniversity of TartuUniversity of TartuTallinn University of TechnologyCompetence Center of Food and Fermentation TechnologiesTallinn University of TechnologyBacteroides thetaiotaomicron is commonly found in the human colon and stabilizes its ecosystem by the catabolism of various polysaccharides. A model of cross-talk between the metabolism of amino acids and fructans in B. thetaiotaomicron was proposed. The growth of B. thetaiotaomicron DSM 2079 in two defined media containing mineral salts and vitamins, and supplemented with either 20 or 2 amino acids, was studied in an isothermal microcalorimeter. The polyfructans inulin (from chicory) and levan (synthesized using levansucrase from Pseudomonas syringae), two fructooligosaccharide preparations with different composition, sucrose and fructose were tested as substrates. The calorimetric power-time curves were substrate specific and typically multiauxic. A surplus of amino acids reduced the consumption of longer oligosaccharides (DP > 3). Bacterial growth was not detected either in the carbohydrate free medium containing amino acids or in the medium with inulin as a sole carbohydrate. In amino acid-restricted medium, fermentation leading to acetic acid formation was dominant at the beginning of growth (up to 24 h), followed by increased lactic acid production, and mainly propionic and succinic acids were produced at the end of fermentation. In the medium supplemented with 20 amino acids, the highest production of D-lactate (82 ± 33 mmol/gDW) occurred in parallel with extensive consumption (up to 17 mmol/gDW) of amino acids, especially Ser, Thr and Asp. The production of Ala and Glu was observed at growth on all substrates, and the production was enhanced under amino acid deficiency. The study revealed the influence of amino acids on fructan metabolism in B. thetaiotaomicron and showed that defined growth media are invaluable in elucidating quantitative metabolic profiles of the bacteria. Levan was shown to act as an easily degradable substrate for B. thetaiotaomicron. The effect of levan on balancing or modifying colon microbiota will be studied in further experiments.http://journal.frontiersin.org/Journal/10.3389/fnut.2014.00021/fullamino acid metabolismMetabolic Flux AnalysisFosfructandefined mediumBacteroides thetaiotaomicron |
| spellingShingle | Signe eAdamberg Katrin eTomson Heiki eVija Marju ePuurand Natalja eKabanova Triinu eVisnapuu Eerik eJõgi Tiina eAlamäe Kaarel eAdamberg Kaarel eAdamberg Kaarel eAdamberg Degradation of fructans and production of propionic acid by Bacteroides thetaiotaomicron are enhanced by shortage of amino acids Frontiers in Nutrition amino acid metabolism Metabolic Flux Analysis Fos fructan defined medium Bacteroides thetaiotaomicron |
| title | Degradation of fructans and production of propionic acid by Bacteroides thetaiotaomicron are enhanced by shortage of amino acids |
| title_full | Degradation of fructans and production of propionic acid by Bacteroides thetaiotaomicron are enhanced by shortage of amino acids |
| title_fullStr | Degradation of fructans and production of propionic acid by Bacteroides thetaiotaomicron are enhanced by shortage of amino acids |
| title_full_unstemmed | Degradation of fructans and production of propionic acid by Bacteroides thetaiotaomicron are enhanced by shortage of amino acids |
| title_short | Degradation of fructans and production of propionic acid by Bacteroides thetaiotaomicron are enhanced by shortage of amino acids |
| title_sort | degradation of fructans and production of propionic acid by bacteroides thetaiotaomicron are enhanced by shortage of amino acids |
| topic | amino acid metabolism Metabolic Flux Analysis Fos fructan defined medium Bacteroides thetaiotaomicron |
| url | http://journal.frontiersin.org/Journal/10.3389/fnut.2014.00021/full |
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