Minimum inhibitory concentration of nano-silver bactericides for beneficial microbes and its effect on Ralstonia solanacearum and seed germination of Japanese Cucumber (Cucumis sativus)
Background Plant growth-promoting rhizobacteria (PGPR) are highly promising biofertilizers that contribute to eco-friendly sustainable agriculture. There have been many reports on the anti-microbial properties of nanoparticles (NPs). Toxic effects of NPs under laboratory conditions have also reporte...
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| Format: | Article |
| Language: | English |
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PeerJ Inc.
2019-03-01
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| Series: | PeerJ |
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| Online Access: | https://peerj.com/articles/6418.pdf |
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| author | Poopak Sotoodehnia Norida Mazlan Halimi Mohd Saud Wahid A. Samsuri Sheikh Hasna Habib Amin Soltangheisi |
| author_facet | Poopak Sotoodehnia Norida Mazlan Halimi Mohd Saud Wahid A. Samsuri Sheikh Hasna Habib Amin Soltangheisi |
| author_sort | Poopak Sotoodehnia |
| collection | DOAJ |
| description | Background Plant growth-promoting rhizobacteria (PGPR) are highly promising biofertilizers that contribute to eco-friendly sustainable agriculture. There have been many reports on the anti-microbial properties of nanoparticles (NPs). Toxic effects of NPs under laboratory conditions have also reported; however, there is a lack of information about their uptake and mobility in organisms under environmental conditions. There is an urgent need to determine the highest concentration of NPs which is not detrimental for growth and proliferation of PGPR. Methods Transmission electron microscopy (TEM) and scanning electron microscopy (SEM) were used to measure the size and shape of NPs. Minimum inhibitory concentrations (MIC) of nano-silver on selected beneficial microbes and Ralstonia solanacearum were measured using the microdilution broth method. The percentage of seed germination was measured under in vitro conditions. Results NPs were spherical with a size of 16 ± 6 nm. Nano-silver at 12–40 mg l−1 inhibited the growth of bacteria. Seed application at 40 mg l−1 protected seeds from R. solanacearum and improved the rate of seed germination. |
| first_indexed | 2024-03-09T07:02:49Z |
| format | Article |
| id | doaj.art-0ba195033dd049c9b44fcee3521fb385 |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T07:02:49Z |
| publishDate | 2019-03-01 |
| publisher | PeerJ Inc. |
| record_format | Article |
| series | PeerJ |
| spelling | doaj.art-0ba195033dd049c9b44fcee3521fb3852023-12-03T09:46:21ZengPeerJ Inc.PeerJ2167-83592019-03-017e641810.7717/peerj.6418Minimum inhibitory concentration of nano-silver bactericides for beneficial microbes and its effect on Ralstonia solanacearum and seed germination of Japanese Cucumber (Cucumis sativus)Poopak Sotoodehnia0Norida Mazlan1Halimi Mohd Saud2Wahid A. Samsuri3Sheikh Hasna Habib4Amin Soltangheisi5Department of Agriculture Technology, Universiti Putra Malaysia, Selangor, MalaysiaDepartment of Agriculture Technology, Universiti Putra Malaysia, Selangor, MalaysiaDepartment of Agriculture Technology, Universiti Putra Malaysia, Selangor, MalaysiaDepartment of Land Management, Faculty of Agriculture, Universiti Putra Malaysia, Selangor, MalaysiaOil Seed Research Center, Bangladesh Agricultural Research Institute, Joydebpur, BangladeshCenter of Nuclear Energy in Agriculture (CENA), University of Sao Paulo, Sao Paulo, BrazilBackground Plant growth-promoting rhizobacteria (PGPR) are highly promising biofertilizers that contribute to eco-friendly sustainable agriculture. There have been many reports on the anti-microbial properties of nanoparticles (NPs). Toxic effects of NPs under laboratory conditions have also reported; however, there is a lack of information about their uptake and mobility in organisms under environmental conditions. There is an urgent need to determine the highest concentration of NPs which is not detrimental for growth and proliferation of PGPR. Methods Transmission electron microscopy (TEM) and scanning electron microscopy (SEM) were used to measure the size and shape of NPs. Minimum inhibitory concentrations (MIC) of nano-silver on selected beneficial microbes and Ralstonia solanacearum were measured using the microdilution broth method. The percentage of seed germination was measured under in vitro conditions. Results NPs were spherical with a size of 16 ± 6 nm. Nano-silver at 12–40 mg l−1 inhibited the growth of bacteria. Seed application at 40 mg l−1 protected seeds from R. solanacearum and improved the rate of seed germination.https://peerj.com/articles/6418.pdfNanoparticlesPGPRSeed germination |
| spellingShingle | Poopak Sotoodehnia Norida Mazlan Halimi Mohd Saud Wahid A. Samsuri Sheikh Hasna Habib Amin Soltangheisi Minimum inhibitory concentration of nano-silver bactericides for beneficial microbes and its effect on Ralstonia solanacearum and seed germination of Japanese Cucumber (Cucumis sativus) PeerJ Nanoparticles PGPR Seed germination |
| title | Minimum inhibitory concentration of nano-silver bactericides for beneficial microbes and its effect on Ralstonia solanacearum and seed germination of Japanese Cucumber (Cucumis sativus) |
| title_full | Minimum inhibitory concentration of nano-silver bactericides for beneficial microbes and its effect on Ralstonia solanacearum and seed germination of Japanese Cucumber (Cucumis sativus) |
| title_fullStr | Minimum inhibitory concentration of nano-silver bactericides for beneficial microbes and its effect on Ralstonia solanacearum and seed germination of Japanese Cucumber (Cucumis sativus) |
| title_full_unstemmed | Minimum inhibitory concentration of nano-silver bactericides for beneficial microbes and its effect on Ralstonia solanacearum and seed germination of Japanese Cucumber (Cucumis sativus) |
| title_short | Minimum inhibitory concentration of nano-silver bactericides for beneficial microbes and its effect on Ralstonia solanacearum and seed germination of Japanese Cucumber (Cucumis sativus) |
| title_sort | minimum inhibitory concentration of nano silver bactericides for beneficial microbes and its effect on ralstonia solanacearum and seed germination of japanese cucumber cucumis sativus |
| topic | Nanoparticles PGPR Seed germination |
| url | https://peerj.com/articles/6418.pdf |
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