Metabolic Characterization of Four Members of the Genus <i>Stachys</i> L. (Lamiaceae)
Several members of <i>Stachys</i> L. (among the largest Lamiaceae genera) have been traditionally used as medicinal plants. With 54 <i>Stachys</i> taxa (species and subspecies) occurring in mainland and/or insular Greece, the present study aimed to investigate the metabolic p...
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MDPI AG
2023-10-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/13/10/2624 |
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| author | Ekaterina-Michaela Tomou Anastasia Karioti Giorgos Tsirogiannidis Nikos Krigas Helen Skaltsa |
| author_facet | Ekaterina-Michaela Tomou Anastasia Karioti Giorgos Tsirogiannidis Nikos Krigas Helen Skaltsa |
| author_sort | Ekaterina-Michaela Tomou |
| collection | DOAJ |
| description | Several members of <i>Stachys</i> L. (among the largest Lamiaceae genera) have been traditionally used as medicinal plants. With 54 <i>Stachys</i> taxa (species and subspecies) occurring in mainland and/or insular Greece, the present study aimed to investigate the metabolic profiling of four range-restricted local <i>Stachys</i> members: <i>Stachys candida</i> and <i>S. chrysantha</i> (protected and endangered local Greek endemics), <i>S. leucoglossa</i> subsp. <i>leucoglossa</i> (local Balkan endemic), and <i>S. spinulosa</i> (local Balkan subendemic). In this investigation, the infusions of their above-ground parts were characterized using NMR and HPLC-PDA-MS techniques. Thus, 1D- and 2D-NMR spectra were obtained to compare the chemical fingerprints of these plants. Furthermore, previously isolated compounds from <i>Stachys</i> spp. were used to identify specific constituents. NMR screening revealed the presence of: (i) phenylethanoid glycosides, mainly acteoside in <i>S. candida</i> and <i>S. chrysantha</i> (section <i>Candida</i>, <i>Swainsoniana</i> phyloclade), and (ii) flavone 7-<i>O</i>-allosylglucoside (isoscutellarein 7-<i>O</i>-[6‴-<i>O</i>-acetyl-β-D-allopyranosyl]-(1→2)-β-D-glucopyranoside) and iridoids (monomelittoside or/and melittoside) in <i>S. leucoglossa</i> subsp. <i>leucoglossa</i> (section <i>Olisia</i>, <i>Swainsoniana/Olisia</i> phyloclade, <i>Swainsoniana</i> phyloclade) and caffeoylquinic acid (chlorogenic acid) in <i>S. spinulosa</i> (section <i>Campanistrum</i>, <i>Stachys</i> phyloclade). In total, 26 compounds were detected by HPLC-PDA-MS belonging to flavonoids, phenylethanoid glycosides, and phenolic acids. Among them, chlorogenic acid was identified in all samples as one of their main metabolites. The present study complements previous studies with first reports of constituents detected in the studied taxa, reports for the first time on the metabolic characterization of <i>S. spinulosa</i>, and discusses the chemotaxonomic significance of such findings. |
| first_indexed | 2024-03-10T21:29:59Z |
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| language | English |
| last_indexed | 2024-03-10T21:29:59Z |
| publishDate | 2023-10-01 |
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| spelling | doaj.art-0ba441b16f744b31b4f5fa77f6e811832023-11-19T15:22:47ZengMDPI AGAgronomy2073-43952023-10-011310262410.3390/agronomy13102624Metabolic Characterization of Four Members of the Genus <i>Stachys</i> L. (Lamiaceae)Ekaterina-Michaela Tomou0Anastasia Karioti1Giorgos Tsirogiannidis2Nikos Krigas3Helen Skaltsa4Department of Pharmacognosy and Chemistry of Natural Products, School of Health Sciences, Faculty of Pharmacy, National and Kapodistrian University of Athens, Panepistimiopolis Zografou, 15771 Athens, GreeceLaboratory of Pharmacognosy, School of Pharmacy, Aristotle University of Thessaloniki, University Campus, 54124 Thessaloniki, GreeceDepartment of Pharmacognosy and Chemistry of Natural Products, School of Health Sciences, Faculty of Pharmacy, National and Kapodistrian University of Athens, Panepistimiopolis Zografou, 15771 Athens, GreeceInstitute of Plant Breeding and Genetic Resources, Agricultural Organization Demeter, P.O. Box 60458, 57001 Thessaloniki, GreeceDepartment of Pharmacognosy and Chemistry of Natural Products, School of Health Sciences, Faculty of Pharmacy, National and Kapodistrian University of Athens, Panepistimiopolis Zografou, 15771 Athens, GreeceSeveral members of <i>Stachys</i> L. (among the largest Lamiaceae genera) have been traditionally used as medicinal plants. With 54 <i>Stachys</i> taxa (species and subspecies) occurring in mainland and/or insular Greece, the present study aimed to investigate the metabolic profiling of four range-restricted local <i>Stachys</i> members: <i>Stachys candida</i> and <i>S. chrysantha</i> (protected and endangered local Greek endemics), <i>S. leucoglossa</i> subsp. <i>leucoglossa</i> (local Balkan endemic), and <i>S. spinulosa</i> (local Balkan subendemic). In this investigation, the infusions of their above-ground parts were characterized using NMR and HPLC-PDA-MS techniques. Thus, 1D- and 2D-NMR spectra were obtained to compare the chemical fingerprints of these plants. Furthermore, previously isolated compounds from <i>Stachys</i> spp. were used to identify specific constituents. NMR screening revealed the presence of: (i) phenylethanoid glycosides, mainly acteoside in <i>S. candida</i> and <i>S. chrysantha</i> (section <i>Candida</i>, <i>Swainsoniana</i> phyloclade), and (ii) flavone 7-<i>O</i>-allosylglucoside (isoscutellarein 7-<i>O</i>-[6‴-<i>O</i>-acetyl-β-D-allopyranosyl]-(1→2)-β-D-glucopyranoside) and iridoids (monomelittoside or/and melittoside) in <i>S. leucoglossa</i> subsp. <i>leucoglossa</i> (section <i>Olisia</i>, <i>Swainsoniana/Olisia</i> phyloclade, <i>Swainsoniana</i> phyloclade) and caffeoylquinic acid (chlorogenic acid) in <i>S. spinulosa</i> (section <i>Campanistrum</i>, <i>Stachys</i> phyloclade). In total, 26 compounds were detected by HPLC-PDA-MS belonging to flavonoids, phenylethanoid glycosides, and phenolic acids. Among them, chlorogenic acid was identified in all samples as one of their main metabolites. The present study complements previous studies with first reports of constituents detected in the studied taxa, reports for the first time on the metabolic characterization of <i>S. spinulosa</i>, and discusses the chemotaxonomic significance of such findings.https://www.mdpi.com/2073-4395/13/10/2624<i>Stachys spinulosa</i>infusionsmetabolic profilingNMRHPLC-PDA-MSGreece |
| spellingShingle | Ekaterina-Michaela Tomou Anastasia Karioti Giorgos Tsirogiannidis Nikos Krigas Helen Skaltsa Metabolic Characterization of Four Members of the Genus <i>Stachys</i> L. (Lamiaceae) Agronomy <i>Stachys spinulosa</i> infusions metabolic profiling NMR HPLC-PDA-MS Greece |
| title | Metabolic Characterization of Four Members of the Genus <i>Stachys</i> L. (Lamiaceae) |
| title_full | Metabolic Characterization of Four Members of the Genus <i>Stachys</i> L. (Lamiaceae) |
| title_fullStr | Metabolic Characterization of Four Members of the Genus <i>Stachys</i> L. (Lamiaceae) |
| title_full_unstemmed | Metabolic Characterization of Four Members of the Genus <i>Stachys</i> L. (Lamiaceae) |
| title_short | Metabolic Characterization of Four Members of the Genus <i>Stachys</i> L. (Lamiaceae) |
| title_sort | metabolic characterization of four members of the genus i stachys i l lamiaceae |
| topic | <i>Stachys spinulosa</i> infusions metabolic profiling NMR HPLC-PDA-MS Greece |
| url | https://www.mdpi.com/2073-4395/13/10/2624 |
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