Dietary exposure of the water flea Daphnia galeata to microcystin-LR
Harmful substances like the cyanotoxin microcystin-leucine-arginine (MC-LR) are commonly found in eutrophic freshwater environments, posing risks to aquatic organisms. The water flea, Daphnia, is a well-established model organism for environmental toxicology research. Nevertheless, there is currentl...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2024-12-01
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| Series: | Animal Cells and Systems |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/19768354.2024.2302529 |
| _version_ | 1826930755979182080 |
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| author | Eun-jeong Kim Donggu Jeon Yeon-jeong Park Hyunmin Woo Seong-il Eyun |
| author_facet | Eun-jeong Kim Donggu Jeon Yeon-jeong Park Hyunmin Woo Seong-il Eyun |
| author_sort | Eun-jeong Kim |
| collection | DOAJ |
| description | Harmful substances like the cyanotoxin microcystin-leucine-arginine (MC-LR) are commonly found in eutrophic freshwater environments, posing risks to aquatic organisms. The water flea, Daphnia, is a well-established model organism for environmental toxicology research. Nevertheless, there is currently insufficient research on the genes that respond to MC-LR in Daphnia galeata. This study aimed to gain insights into the notable genes that react significantly to MC-LR. In this study, we generated an extensive RNA-Seq sequences isolated from the D. galeata HK strain, Han River in Korea. This strain was nourished with a diet of the green microalga Chlorella vulgaris and treated with pure MC-LR at a concentration of 36 ug/L. The transcriptome profile in response to the MC-LR treatment was obtained and 336 differentially expressed genes were subjected to Gene Ontology (GO) and euKaryotic Orthologous Groups of proteins analyses. GO enrichment analysis showed that chemical stimulus, amino sugar metabolic and catabolic process, oxidative stress, and detoxification were highly enriched, in reverse, proteolysis and fucosylation were underpresented. Detoxification process related genes such as peroxidase-like, chorion, and thyroid peroxidase-like were enriched for eliminating or neutralizing MC_LR from an organism's body. Furthermore, functional protein classification revealed an upregulation of lipid and inorganic ion transport processes, while amino acid and carbohydrate transport processes were found to be downregulated. These findings offer insights into how organisms respond to ecotoxic stimuli, providing valuable information for understanding adaptation or defense pathways. |
| first_indexed | 2024-03-08T09:41:24Z |
| format | Article |
| id | doaj.art-0ba5229bb6a64904b2226bbcd1b321b7 |
| institution | Directory Open Access Journal |
| issn | 1976-8354 2151-2485 |
| language | English |
| last_indexed | 2025-02-17T16:33:22Z |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Animal Cells and Systems |
| spelling | doaj.art-0ba5229bb6a64904b2226bbcd1b321b72024-12-17T00:08:34ZengTaylor & Francis GroupAnimal Cells and Systems1976-83542151-24852024-12-01281253610.1080/19768354.2024.2302529Dietary exposure of the water flea Daphnia galeata to microcystin-LREun-jeong Kim0Donggu Jeon1Yeon-jeong Park2Hyunmin Woo3Seong-il Eyun4Department of Life Science, Chung-ang University, Seoul, KoreaDepartment of Life Science, Chung-ang University, Seoul, KoreaWater Environmental Management Department, Korea Water Resources Corporation (K-water), Daejeon, KoreaDepartment of Life Science, Chung-ang University, Seoul, KoreaDepartment of Life Science, Chung-ang University, Seoul, KoreaHarmful substances like the cyanotoxin microcystin-leucine-arginine (MC-LR) are commonly found in eutrophic freshwater environments, posing risks to aquatic organisms. The water flea, Daphnia, is a well-established model organism for environmental toxicology research. Nevertheless, there is currently insufficient research on the genes that respond to MC-LR in Daphnia galeata. This study aimed to gain insights into the notable genes that react significantly to MC-LR. In this study, we generated an extensive RNA-Seq sequences isolated from the D. galeata HK strain, Han River in Korea. This strain was nourished with a diet of the green microalga Chlorella vulgaris and treated with pure MC-LR at a concentration of 36 ug/L. The transcriptome profile in response to the MC-LR treatment was obtained and 336 differentially expressed genes were subjected to Gene Ontology (GO) and euKaryotic Orthologous Groups of proteins analyses. GO enrichment analysis showed that chemical stimulus, amino sugar metabolic and catabolic process, oxidative stress, and detoxification were highly enriched, in reverse, proteolysis and fucosylation were underpresented. Detoxification process related genes such as peroxidase-like, chorion, and thyroid peroxidase-like were enriched for eliminating or neutralizing MC_LR from an organism's body. Furthermore, functional protein classification revealed an upregulation of lipid and inorganic ion transport processes, while amino acid and carbohydrate transport processes were found to be downregulated. These findings offer insights into how organisms respond to ecotoxic stimuli, providing valuable information for understanding adaptation or defense pathways.https://www.tandfonline.com/doi/10.1080/19768354.2024.2302529Daphnia galeataMC-LRdetoxificationoxidative stressgene expression |
| spellingShingle | Eun-jeong Kim Donggu Jeon Yeon-jeong Park Hyunmin Woo Seong-il Eyun Dietary exposure of the water flea Daphnia galeata to microcystin-LR Animal Cells and Systems Daphnia galeata MC-LR detoxification oxidative stress gene expression |
| title | Dietary exposure of the water flea Daphnia galeata to microcystin-LR |
| title_full | Dietary exposure of the water flea Daphnia galeata to microcystin-LR |
| title_fullStr | Dietary exposure of the water flea Daphnia galeata to microcystin-LR |
| title_full_unstemmed | Dietary exposure of the water flea Daphnia galeata to microcystin-LR |
| title_short | Dietary exposure of the water flea Daphnia galeata to microcystin-LR |
| title_sort | dietary exposure of the water flea daphnia galeata to microcystin lr |
| topic | Daphnia galeata MC-LR detoxification oxidative stress gene expression |
| url | https://www.tandfonline.com/doi/10.1080/19768354.2024.2302529 |
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