Development of an Impedimetric Aptasensor for Label Free Detection of Patulin in Apple Juice
In the present work, an aptasensing platform was developed for the detection of a carcinogenic mycotoxin termed patulin (PAT) using a label-free approach. The detection was mainly based on a specific interaction of an aptamer immobilized on carbon-based electrode. A long linear spacer of carboxy-ami...
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-03-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/24/6/1017 |
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| author | Reem Khan Sondes Ben Aissa Tauqir A. Sherazi Gaelle Catanante Akhtar Hayat Jean Louis Marty |
| author_facet | Reem Khan Sondes Ben Aissa Tauqir A. Sherazi Gaelle Catanante Akhtar Hayat Jean Louis Marty |
| author_sort | Reem Khan |
| collection | DOAJ |
| description | In the present work, an aptasensing platform was developed for the detection of a carcinogenic mycotoxin termed patulin (PAT) using a label-free approach. The detection was mainly based on a specific interaction of an aptamer immobilized on carbon-based electrode. A long linear spacer of carboxy-amine polyethylene glycol chain (PEG) was chemically grafted on screen-printed carbon electrodes (SPCEs) via diazonium salt in the aptasensor design. The NH2-modified aptamer was then attached covalently to carboxylic acid groups of previously immobilized bifunctional PEG to build a diblock macromolecule. The immobilized diblocked molecules resulted in the formation of long tunnels on a carbon interface, while the aptamer was assumed as the gate of these tunnels. Upon target analyte binding, the gates were assumed to be closed due to conformational changes in the structure of the aptamer, increasing the resistance to the charge transfer. This increase in resistance was measured by electrochemical impedance spectroscopy, the main analytical technique for the quantitative detection of PAT. Encouragingly, a good linear range between 1 and 25 ng was obtained. The limit of detection and limit of quantification was 2.8 ng L−1 and 4.0 ng L−1, respectively. Selectivity of the aptasensor was confirmed with mycotoxins commonly occurring in food. The developed apta-assay was also applied to a real sample, i.e., fresh apple juice spiked with PAT, and toxin recovery up to 99% was observed. The results obtained validated the suitability and selectivity of the developed apta-assay for the identification and quantification of PAT in real food samples. |
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| id | doaj.art-0ba6282ec80a40d49609d4c3e3b898f8 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-24T00:06:28Z |
| publishDate | 2019-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-0ba6282ec80a40d49609d4c3e3b898f82022-12-21T17:25:00ZengMDPI AGMolecules1420-30492019-03-01246101710.3390/molecules24061017molecules24061017Development of an Impedimetric Aptasensor for Label Free Detection of Patulin in Apple JuiceReem Khan0Sondes Ben Aissa1Tauqir A. Sherazi2Gaelle Catanante3Akhtar Hayat4Jean Louis Marty5BAE: Biocapteurs-Analyses-Environnement, Universite de Perpignan Via Domitia, 52 Avenue Paul Alduy, 66860 Perpignan CEDEX, FranceBAE: Biocapteurs-Analyses-Environnement, Universite de Perpignan Via Domitia, 52 Avenue Paul Alduy, 66860 Perpignan CEDEX, FranceDepartment of chemistry, COMSATS University Islamabad, Abbottabad Campus, Abbottabad 22060, PakistanBAE: Biocapteurs-Analyses-Environnement, Universite de Perpignan Via Domitia, 52 Avenue Paul Alduy, 66860 Perpignan CEDEX, FranceInterdisciplinary Research Centre in Biomedical Materials (IRCBM), COMSATS University Islamabad, Lahore Campus, Lahore 54000, PakistanBAE: Biocapteurs-Analyses-Environnement, Universite de Perpignan Via Domitia, 52 Avenue Paul Alduy, 66860 Perpignan CEDEX, FranceIn the present work, an aptasensing platform was developed for the detection of a carcinogenic mycotoxin termed patulin (PAT) using a label-free approach. The detection was mainly based on a specific interaction of an aptamer immobilized on carbon-based electrode. A long linear spacer of carboxy-amine polyethylene glycol chain (PEG) was chemically grafted on screen-printed carbon electrodes (SPCEs) via diazonium salt in the aptasensor design. The NH2-modified aptamer was then attached covalently to carboxylic acid groups of previously immobilized bifunctional PEG to build a diblock macromolecule. The immobilized diblocked molecules resulted in the formation of long tunnels on a carbon interface, while the aptamer was assumed as the gate of these tunnels. Upon target analyte binding, the gates were assumed to be closed due to conformational changes in the structure of the aptamer, increasing the resistance to the charge transfer. This increase in resistance was measured by electrochemical impedance spectroscopy, the main analytical technique for the quantitative detection of PAT. Encouragingly, a good linear range between 1 and 25 ng was obtained. The limit of detection and limit of quantification was 2.8 ng L−1 and 4.0 ng L−1, respectively. Selectivity of the aptasensor was confirmed with mycotoxins commonly occurring in food. The developed apta-assay was also applied to a real sample, i.e., fresh apple juice spiked with PAT, and toxin recovery up to 99% was observed. The results obtained validated the suitability and selectivity of the developed apta-assay for the identification and quantification of PAT in real food samples.http://www.mdpi.com/1420-3049/24/6/1017impedimetric aptasensorscreen-printed interfacebifunctional polymer armsPAT detectionapple juice |
| spellingShingle | Reem Khan Sondes Ben Aissa Tauqir A. Sherazi Gaelle Catanante Akhtar Hayat Jean Louis Marty Development of an Impedimetric Aptasensor for Label Free Detection of Patulin in Apple Juice Molecules impedimetric aptasensor screen-printed interface bifunctional polymer arms PAT detection apple juice |
| title | Development of an Impedimetric Aptasensor for Label Free Detection of Patulin in Apple Juice |
| title_full | Development of an Impedimetric Aptasensor for Label Free Detection of Patulin in Apple Juice |
| title_fullStr | Development of an Impedimetric Aptasensor for Label Free Detection of Patulin in Apple Juice |
| title_full_unstemmed | Development of an Impedimetric Aptasensor for Label Free Detection of Patulin in Apple Juice |
| title_short | Development of an Impedimetric Aptasensor for Label Free Detection of Patulin in Apple Juice |
| title_sort | development of an impedimetric aptasensor for label free detection of patulin in apple juice |
| topic | impedimetric aptasensor screen-printed interface bifunctional polymer arms PAT detection apple juice |
| url | http://www.mdpi.com/1420-3049/24/6/1017 |
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