Identificatin and quantiative analysis of amyrins in Humulus lupulus L.
In the paper, the identification and quantification of α- and β-amyrin in five varieties of Humulus lupulus is described. The plant samples were extracted with ethyl acetate and analyzed using of high performance liquid chromatography (HPLC) with PAD detection. The separation was achieved on RP 18 c...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Sciendo
2014-09-01
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| Series: | Current Issues in Pharmacy and Medical Sciences |
| Subjects: | |
| Online Access: | https://doi.org/10.1515/cipms-2015-0002 |
| _version_ | 1818720292691771392 |
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| author | Rokicka Kamila Wojciak-Kosior Magdalena |
| author_facet | Rokicka Kamila Wojciak-Kosior Magdalena |
| author_sort | Rokicka Kamila |
| collection | DOAJ |
| description | In the paper, the identification and quantification of α- and β-amyrin in five varieties of Humulus lupulus is described. The plant samples were extracted with ethyl acetate and analyzed using of high performance liquid chromatography (HPLC) with PAD detection. The separation was achieved on RP 18 column, at 2 mL/min flow rate and at temperature of 35°C. Acetonitrile was used as a mobile phase. The established calibration curves and the other validation parameters: linearity (correlation coefficient r > 0.9988) and precision (RSD values ranged from 0.14 to 1.81%) were found to be satisfactory for the proposed method. The content of α- and β-amyrin strongly depended on varieties of H. lupulus and amounted from 72.7 to 232.5 μg/g and from 77.9 to 176.9 μg/g of dry plant material, respectively. |
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| format | Article |
| id | doaj.art-0ba8fb17e2db4af4a9bec8299bfcfd74 |
| institution | Directory Open Access Journal |
| issn | 2084-980X 2300-6676 |
| language | English |
| last_indexed | 2024-12-17T20:20:31Z |
| publishDate | 2014-09-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Current Issues in Pharmacy and Medical Sciences |
| spelling | doaj.art-0ba8fb17e2db4af4a9bec8299bfcfd742022-12-21T21:33:57ZengSciendoCurrent Issues in Pharmacy and Medical Sciences2084-980X2300-66762014-09-0127314214410.1515/cipms-2015-0002cipms-2015-0002Identificatin and quantiative analysis of amyrins in Humulus lupulus L.Rokicka Kamila0Wojciak-Kosior Magdalena1Departament of Chemistry, Medicinal University in Lublin, Chodzki 4a, 20-093 LublinDepartament of Chemistry, Medicinal University in Lublin, Chodzki 4a, 20-093 LublinIn the paper, the identification and quantification of α- and β-amyrin in five varieties of Humulus lupulus is described. The plant samples were extracted with ethyl acetate and analyzed using of high performance liquid chromatography (HPLC) with PAD detection. The separation was achieved on RP 18 column, at 2 mL/min flow rate and at temperature of 35°C. Acetonitrile was used as a mobile phase. The established calibration curves and the other validation parameters: linearity (correlation coefficient r > 0.9988) and precision (RSD values ranged from 0.14 to 1.81%) were found to be satisfactory for the proposed method. The content of α- and β-amyrin strongly depended on varieties of H. lupulus and amounted from 72.7 to 232.5 μg/g and from 77.9 to 176.9 μg/g of dry plant material, respectively.https://doi.org/10.1515/cipms-2015-0002amyrinhumulus lupulustriterpenes |
| spellingShingle | Rokicka Kamila Wojciak-Kosior Magdalena Identificatin and quantiative analysis of amyrins in Humulus lupulus L. Current Issues in Pharmacy and Medical Sciences amyrin humulus lupulus triterpenes |
| title | Identificatin and quantiative analysis of amyrins in Humulus lupulus L. |
| title_full | Identificatin and quantiative analysis of amyrins in Humulus lupulus L. |
| title_fullStr | Identificatin and quantiative analysis of amyrins in Humulus lupulus L. |
| title_full_unstemmed | Identificatin and quantiative analysis of amyrins in Humulus lupulus L. |
| title_short | Identificatin and quantiative analysis of amyrins in Humulus lupulus L. |
| title_sort | identificatin and quantiative analysis of amyrins in humulus lupulus l |
| topic | amyrin humulus lupulus triterpenes |
| url | https://doi.org/10.1515/cipms-2015-0002 |
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