Detection of Creatinine in Vaginal Secretions of Women with Premature Rupture of Membranes in Third Trimester from Semiurban Population: A Cross-sectional Study
Introduction: Spontaneous rupture of the membranes any time beyond the 28th week of pregnancy but before the onset of labour is called Premature Rupture of Membranes (PROM). There are many techniques for diagnosing PROM that is based on both clinical assessments and biological studies. Each of these...
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JCDR Research and Publications Private Limited
2023-01-01
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| Series: | Journal of Clinical and Diagnostic Research |
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| author | Hemant Gopalrao Deshpande Monalisa Sarkar Madhukar Jagannath Shinde Chetan Gulati Shubham Joon |
| author_facet | Hemant Gopalrao Deshpande Monalisa Sarkar Madhukar Jagannath Shinde Chetan Gulati Shubham Joon |
| author_sort | Hemant Gopalrao Deshpande |
| collection | DOAJ |
| description | Introduction: Spontaneous rupture of the membranes any time beyond the 28th week of pregnancy but before the onset of labour is called Premature Rupture of Membranes (PROM). There are many techniques for diagnosing PROM that is based on both clinical assessments and biological studies. Each of these procedures has benefits and drawbacks. Since foetal urine is the major source of amniotic fluid, the presence of vagina and a high creatinine level may aid in the diagnosis of PROM.
Aim: To determine the cut-off levels of serum creatinine in predicting the PROM.
Materials and Methods: A cross-sectional study was conducted at Dr. DY Patil Medical College, Hospital and Research Centre in Pune, Maharashtra, India from June 1 2020 to June 5 2021. Two equal groups of 140 each pregnant women with gestational ages ranging from 28-42 weeks were created: one with a history of vaginal leaking (the study group, P), and the other with gestationally matched no leakage (the control group, C). Patient’s details such as age, gestational age, and obstetric history were noted. Each patient was examined and cervicovaginal secretions were collected. A 5 mL of saline was flushed in the posterior fornix of vagina. This fluid with vaginal wash was aspirated and creatinine concentration from vaginal fluid was determined by Jaffee synthetic chemical colorimetric method. The Student’s t-test and the Chi-square test were used to analyse the data.
Results: The study group’s vaginal fluid creatinine level was substantially higher (p-value<0.001). The cut-off value of 0.6 mg/dL had highest sensitivity 96.1% and specificity 100%. The area under the Receiver Operating Characteristics (ROC) curve was 0.917. The Positive Predictive Value (PPV) and Negative Predictive Value (NPV) of serum creatinine at cut-off 0.6 mg/dL was 100% and 96.3% respectively.
Conclusion: Vaginal fluid creatinine is simple and easily available, which makes it acceptable to the candidate for a diagnosis of PROM. |
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| spelling | doaj.art-0bab87b96e1d459e8240e6eb6f4d5d662023-02-02T06:46:23ZengJCDR Research and Publications Private LimitedJournal of Clinical and Diagnostic Research2249-782X0973-709X2023-01-01171QC01QC0410.7860/JCDR/2023/56744.17346Detection of Creatinine in Vaginal Secretions of Women with Premature Rupture of Membranes in Third Trimester from Semiurban Population: A Cross-sectional StudyHemant Gopalrao Deshpande0Monalisa Sarkar1Madhukar Jagannath Shinde2Chetan Gulati3Shubham Joon4Head, Department of Obstetrics and Gynaecology, Dr DY Patil Medical College, Hospital and Research Centre, Pune, Maharashtra, India.Resident, Department of Obstetrics and Gynaecology, Dr DY Patil Medical College, hospital and Research Centre, Pune, Maharashtra, India.Assistant Professor, Department of Obstetrics and Gynaecology, Dr DY Patil Medical College, Hospital and Research Centre, Pune, Maharashtra, India.Resident, Department of Obstetrics and Gynaecology, Dr DY Patil Medical College, Hospital and Research Centre, Pune, Maharashtra, India.Student, Department of Obstetrics and Gynaecology, Dr DY Patil Medical College, Hospital and Research Centre, Pune, Maharashtra, India.Introduction: Spontaneous rupture of the membranes any time beyond the 28th week of pregnancy but before the onset of labour is called Premature Rupture of Membranes (PROM). There are many techniques for diagnosing PROM that is based on both clinical assessments and biological studies. Each of these procedures has benefits and drawbacks. Since foetal urine is the major source of amniotic fluid, the presence of vagina and a high creatinine level may aid in the diagnosis of PROM. Aim: To determine the cut-off levels of serum creatinine in predicting the PROM. Materials and Methods: A cross-sectional study was conducted at Dr. DY Patil Medical College, Hospital and Research Centre in Pune, Maharashtra, India from June 1 2020 to June 5 2021. Two equal groups of 140 each pregnant women with gestational ages ranging from 28-42 weeks were created: one with a history of vaginal leaking (the study group, P), and the other with gestationally matched no leakage (the control group, C). Patient’s details such as age, gestational age, and obstetric history were noted. Each patient was examined and cervicovaginal secretions were collected. A 5 mL of saline was flushed in the posterior fornix of vagina. This fluid with vaginal wash was aspirated and creatinine concentration from vaginal fluid was determined by Jaffee synthetic chemical colorimetric method. The Student’s t-test and the Chi-square test were used to analyse the data. Results: The study group’s vaginal fluid creatinine level was substantially higher (p-value<0.001). The cut-off value of 0.6 mg/dL had highest sensitivity 96.1% and specificity 100%. The area under the Receiver Operating Characteristics (ROC) curve was 0.917. The Positive Predictive Value (PPV) and Negative Predictive Value (NPV) of serum creatinine at cut-off 0.6 mg/dL was 100% and 96.3% respectively. Conclusion: Vaginal fluid creatinine is simple and easily available, which makes it acceptable to the candidate for a diagnosis of PROM. https://www.jcdr.net/articles/PDF/17346/56744_CE(AD)_F(KM)_PF1(AG_KM)_PFA(AG_KM)_PN(KM).pdfamniotic membranefetoproteinfibronectingonadotropinprelabourprolactin |
| spellingShingle | Hemant Gopalrao Deshpande Monalisa Sarkar Madhukar Jagannath Shinde Chetan Gulati Shubham Joon Detection of Creatinine in Vaginal Secretions of Women with Premature Rupture of Membranes in Third Trimester from Semiurban Population: A Cross-sectional Study Journal of Clinical and Diagnostic Research amniotic membrane fetoprotein fibronectin gonadotropin prelabour prolactin |
| title | Detection of Creatinine in Vaginal Secretions of Women with Premature Rupture of Membranes in Third Trimester from Semiurban Population: A Cross-sectional Study |
| title_full | Detection of Creatinine in Vaginal Secretions of Women with Premature Rupture of Membranes in Third Trimester from Semiurban Population: A Cross-sectional Study |
| title_fullStr | Detection of Creatinine in Vaginal Secretions of Women with Premature Rupture of Membranes in Third Trimester from Semiurban Population: A Cross-sectional Study |
| title_full_unstemmed | Detection of Creatinine in Vaginal Secretions of Women with Premature Rupture of Membranes in Third Trimester from Semiurban Population: A Cross-sectional Study |
| title_short | Detection of Creatinine in Vaginal Secretions of Women with Premature Rupture of Membranes in Third Trimester from Semiurban Population: A Cross-sectional Study |
| title_sort | detection of creatinine in vaginal secretions of women with premature rupture of membranes in third trimester from semiurban population a cross sectional study |
| topic | amniotic membrane fetoprotein fibronectin gonadotropin prelabour prolactin |
| url | https://www.jcdr.net/articles/PDF/17346/56744_CE(AD)_F(KM)_PF1(AG_KM)_PFA(AG_KM)_PN(KM).pdf |
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