Particle Number of Aerosol in Specific Conditions of Biotechnology Laboratory Cleanroom
This paper presents the results of experimental measurements of the number of aerosol particles in cleanroom air of a biotechnology laboratory for DNA analysis. The aerosol content in the air can harm the accuracy of any analysis performed. The experimental measurements demonstrate compliance with t...
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MDPI AG
2023-05-01
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| Series: | Applied Sciences |
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| Online Access: | https://www.mdpi.com/2076-3417/13/10/5915 |
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| author | Petr Junga Petr Trávníček Tomáš Vítěz Gabrielle Machů Juraj Ružbarský |
| author_facet | Petr Junga Petr Trávníček Tomáš Vítěz Gabrielle Machů Juraj Ružbarský |
| author_sort | Petr Junga |
| collection | DOAJ |
| description | This paper presents the results of experimental measurements of the number of aerosol particles in cleanroom air of a biotechnology laboratory for DNA analysis. The aerosol content in the air can harm the accuracy of any analysis performed. The experimental measurements demonstrate compliance with the ISO cleanroom requirements for class 7. The indoor environment of the investigated biotechnology laboratory cleanroom can be classified as ISO cleanroom class 8. The particle count values depend on the month in which the measurement is made. The location of the measurement site, on the other hand, does not have too much influence. Our results showed that the values of particle number in the laboratory ranged from 325,965 to 2,740,877 pc/m<sup>3</sup> for particles of 0.5 μm and more. From these measurements, it can be concluded that the biotechnology laboratory premises were not very suitable locations to perform for the intended DNA tests since microbiological contamination had the capacity to distort the results of these tests. As such, the authors propose several strategies to improve the current situation. These strategies are based on changing the laboratory work organization and cleaning methodology. In addition, the monitoring of particles with a size of 5 µm or more is recommended. |
| first_indexed | 2024-03-11T03:58:17Z |
| format | Article |
| id | doaj.art-0bb00eb9e953499aaae896e1bc4e314f |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-11T03:58:17Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
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| series | Applied Sciences |
| spelling | doaj.art-0bb00eb9e953499aaae896e1bc4e314f2023-11-18T00:17:40ZengMDPI AGApplied Sciences2076-34172023-05-011310591510.3390/app13105915Particle Number of Aerosol in Specific Conditions of Biotechnology Laboratory CleanroomPetr Junga0Petr Trávníček1Tomáš Vítěz2Gabrielle Machů3Juraj Ružbarský4Department of Agricultural, Food and Environmental Engineering, Faculty of AgriSciences, Mendel University in Brno, 61300 Brno, Czech RepublicDepartment of Agricultural, Food and Environmental Engineering, Faculty of AgriSciences, Mendel University in Brno, 61300 Brno, Czech RepublicDepartment of Agricultural, Food and Environmental Engineering, Faculty of AgriSciences, Mendel University in Brno, 61300 Brno, Czech RepublicDepartment of Agricultural, Food and Environmental Engineering, Faculty of AgriSciences, Mendel University in Brno, 61300 Brno, Czech RepublicFaculty of Manufacturing Technologies, Technical University of Kosice with a Seat in Presov, 080 01 Presov, SlovakiaThis paper presents the results of experimental measurements of the number of aerosol particles in cleanroom air of a biotechnology laboratory for DNA analysis. The aerosol content in the air can harm the accuracy of any analysis performed. The experimental measurements demonstrate compliance with the ISO cleanroom requirements for class 7. The indoor environment of the investigated biotechnology laboratory cleanroom can be classified as ISO cleanroom class 8. The particle count values depend on the month in which the measurement is made. The location of the measurement site, on the other hand, does not have too much influence. Our results showed that the values of particle number in the laboratory ranged from 325,965 to 2,740,877 pc/m<sup>3</sup> for particles of 0.5 μm and more. From these measurements, it can be concluded that the biotechnology laboratory premises were not very suitable locations to perform for the intended DNA tests since microbiological contamination had the capacity to distort the results of these tests. As such, the authors propose several strategies to improve the current situation. These strategies are based on changing the laboratory work organization and cleaning methodology. In addition, the monitoring of particles with a size of 5 µm or more is recommended.https://www.mdpi.com/2076-3417/13/10/5915cleanroomparticulate contaminationaerosolfiltration |
| spellingShingle | Petr Junga Petr Trávníček Tomáš Vítěz Gabrielle Machů Juraj Ružbarský Particle Number of Aerosol in Specific Conditions of Biotechnology Laboratory Cleanroom Applied Sciences cleanroom particulate contamination aerosol filtration |
| title | Particle Number of Aerosol in Specific Conditions of Biotechnology Laboratory Cleanroom |
| title_full | Particle Number of Aerosol in Specific Conditions of Biotechnology Laboratory Cleanroom |
| title_fullStr | Particle Number of Aerosol in Specific Conditions of Biotechnology Laboratory Cleanroom |
| title_full_unstemmed | Particle Number of Aerosol in Specific Conditions of Biotechnology Laboratory Cleanroom |
| title_short | Particle Number of Aerosol in Specific Conditions of Biotechnology Laboratory Cleanroom |
| title_sort | particle number of aerosol in specific conditions of biotechnology laboratory cleanroom |
| topic | cleanroom particulate contamination aerosol filtration |
| url | https://www.mdpi.com/2076-3417/13/10/5915 |
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