Postnatal Right Ventricular Developmental Track Changed by Volume Overload
Background Current right ventricular (RV) volume overload (VO) is established in adult mice. There are no neonatal mouse VO models and how VO affects postnatal RV development is largely unknown. Methods and Results Neonatal VO was induced by the fistula between abdominal aorta and inferior vena cava...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2021-08-01
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| Series: | Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease |
| Subjects: | |
| Online Access: | https://www.ahajournals.org/doi/10.1161/JAHA.121.020854 |
| _version_ | 1797810160254582784 |
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| author | Sijuan Sun Yuqing Hu Yingying Xiao Shoubao Wang Chuan Jiang Jinfen Liu Hao Zhang Haifa Hong Fen Li Lincai Ye |
| author_facet | Sijuan Sun Yuqing Hu Yingying Xiao Shoubao Wang Chuan Jiang Jinfen Liu Hao Zhang Haifa Hong Fen Li Lincai Ye |
| author_sort | Sijuan Sun |
| collection | DOAJ |
| description | Background Current right ventricular (RV) volume overload (VO) is established in adult mice. There are no neonatal mouse VO models and how VO affects postnatal RV development is largely unknown. Methods and Results Neonatal VO was induced by the fistula between abdominal aorta and inferior vena cava on postnatal day 7 and confirmed by abdominal ultrasound, echocardiography, and hematoxylin and eosin staining. The RNA‐sequencing results showed that the top 5 most enriched gene ontology terms in normal RV development were energy derivation by oxidation of organic compounds, generation of precursor metabolites and energy, cellular respiration, striated muscle tissue development, and muscle organ development. Under the influence of VO, the top 5 most enriched gene ontology terms were angiogenesis, regulation of cytoskeleton organization, regulation of vasculature development, regulation of mitotic cell cycle, and regulation of the actin filament‐based process. The top 3 enriched signaling pathways for the normal RV development were PPAR signaling pathway, citrate cycle (Tricarboxylic acid cycle), and fatty acid degradation. VO changed the signaling pathways to focal adhesion, the PI3K‐Akt signaling pathway, and pathways in cancer. The RNA sequencing results were confirmed by the examination of the markers of metabolic and cardiac muscle maturation and the markers of cell cycle and angiogenesis. Conclusions A neonatal mouse VO model was successfully established, and the main processes of postnatal RV development were metabolic and cardiac muscle maturation, and VO changed that to angiogenesis and cell cycle regulation. |
| first_indexed | 2024-03-13T07:05:04Z |
| format | Article |
| id | doaj.art-0bb1118a8a924178953dec3a0b101ab1 |
| institution | Directory Open Access Journal |
| issn | 2047-9980 |
| language | English |
| last_indexed | 2024-03-13T07:05:04Z |
| publishDate | 2021-08-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease |
| spelling | doaj.art-0bb1118a8a924178953dec3a0b101ab12023-06-06T12:10:51ZengWileyJournal of the American Heart Association: Cardiovascular and Cerebrovascular Disease2047-99802021-08-01101610.1161/JAHA.121.020854Postnatal Right Ventricular Developmental Track Changed by Volume OverloadSijuan Sun0Yuqing Hu1Yingying Xiao2Shoubao Wang3Chuan Jiang4Jinfen Liu5Hao Zhang6Haifa Hong7Fen Li8Lincai Ye9Department of Pediatric Intensive Care Unit Shanghai Children's Medical Center School of Medicine Shanghai Jiao Tong University Shanghai ChinaDepartment of Cardiology, Shanghai Children's Medical Center, School of Medicine Shanghai Jiao Tong University Shanghai ChinaDepartment of Thoracic and Cardiovascular Surgery, Shanghai Children's Medical Center, School of Medicine Shanghai Jiao Tong University Shanghai ChinaDepartment of Plastic and Reconstructive Surgery Shanghai Ninth People's Hospital School of Medicine Shanghai Jiao Tong University Shanghai ChinaDepartment of Thoracic and Cardiovascular Surgery, Shanghai Children's Medical Center, School of Medicine Shanghai Jiao Tong University Shanghai ChinaDepartment of Thoracic and Cardiovascular Surgery, Shanghai Children's Medical Center, School of Medicine Shanghai Jiao Tong University Shanghai ChinaDepartment of Thoracic and Cardiovascular Surgery, Shanghai Children's Medical Center, School of Medicine Shanghai Jiao Tong University Shanghai ChinaShanghai Institute for Pediatric Congenital Heart Disease Shanghai Children's Medical Center School of Medicine Shanghai Jiao Tong University Shanghai ChinaDepartment of Cardiology, Shanghai Children's Medical Center, School of Medicine Shanghai Jiao Tong University Shanghai ChinaDepartment of Thoracic and Cardiovascular Surgery, Shanghai Children's Medical Center, School of Medicine Shanghai Jiao Tong University Shanghai ChinaBackground Current right ventricular (RV) volume overload (VO) is established in adult mice. There are no neonatal mouse VO models and how VO affects postnatal RV development is largely unknown. Methods and Results Neonatal VO was induced by the fistula between abdominal aorta and inferior vena cava on postnatal day 7 and confirmed by abdominal ultrasound, echocardiography, and hematoxylin and eosin staining. The RNA‐sequencing results showed that the top 5 most enriched gene ontology terms in normal RV development were energy derivation by oxidation of organic compounds, generation of precursor metabolites and energy, cellular respiration, striated muscle tissue development, and muscle organ development. Under the influence of VO, the top 5 most enriched gene ontology terms were angiogenesis, regulation of cytoskeleton organization, regulation of vasculature development, regulation of mitotic cell cycle, and regulation of the actin filament‐based process. The top 3 enriched signaling pathways for the normal RV development were PPAR signaling pathway, citrate cycle (Tricarboxylic acid cycle), and fatty acid degradation. VO changed the signaling pathways to focal adhesion, the PI3K‐Akt signaling pathway, and pathways in cancer. The RNA sequencing results were confirmed by the examination of the markers of metabolic and cardiac muscle maturation and the markers of cell cycle and angiogenesis. Conclusions A neonatal mouse VO model was successfully established, and the main processes of postnatal RV development were metabolic and cardiac muscle maturation, and VO changed that to angiogenesis and cell cycle regulation.https://www.ahajournals.org/doi/10.1161/JAHA.121.020854cardiomyocyteproliferationright ventricleRNA sequencingvolume overload |
| spellingShingle | Sijuan Sun Yuqing Hu Yingying Xiao Shoubao Wang Chuan Jiang Jinfen Liu Hao Zhang Haifa Hong Fen Li Lincai Ye Postnatal Right Ventricular Developmental Track Changed by Volume Overload Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease cardiomyocyte proliferation right ventricle RNA sequencing volume overload |
| title | Postnatal Right Ventricular Developmental Track Changed by Volume Overload |
| title_full | Postnatal Right Ventricular Developmental Track Changed by Volume Overload |
| title_fullStr | Postnatal Right Ventricular Developmental Track Changed by Volume Overload |
| title_full_unstemmed | Postnatal Right Ventricular Developmental Track Changed by Volume Overload |
| title_short | Postnatal Right Ventricular Developmental Track Changed by Volume Overload |
| title_sort | postnatal right ventricular developmental track changed by volume overload |
| topic | cardiomyocyte proliferation right ventricle RNA sequencing volume overload |
| url | https://www.ahajournals.org/doi/10.1161/JAHA.121.020854 |
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