A single-cell atlas of the cycling murine ovary
The estrous cycle is regulated by rhythmic endocrine interactions of the nervous and reproductive systems, which coordinate the hormonal and ovulatory functions of the ovary. Folliculogenesis and follicle progression require the orchestrated response of a variety of cell types to allow the maturatio...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
eLife Sciences Publications Ltd
2022-10-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/77239 |
| _version_ | 1797996463880404992 |
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| author | Mary E Morris Marie-Charlotte Meinsohn Maeva Chauvin Hatice D Saatcioglu Aki Kashiwagi Natalie A Sicher Ngoc Nguyen Selena Yuan Rhian Stavely Minsuk Hyun Patricia K Donahoe Bernardo L Sabatini David Pépin |
| author_facet | Mary E Morris Marie-Charlotte Meinsohn Maeva Chauvin Hatice D Saatcioglu Aki Kashiwagi Natalie A Sicher Ngoc Nguyen Selena Yuan Rhian Stavely Minsuk Hyun Patricia K Donahoe Bernardo L Sabatini David Pépin |
| author_sort | Mary E Morris |
| collection | DOAJ |
| description | The estrous cycle is regulated by rhythmic endocrine interactions of the nervous and reproductive systems, which coordinate the hormonal and ovulatory functions of the ovary. Folliculogenesis and follicle progression require the orchestrated response of a variety of cell types to allow the maturation of the follicle and its sequela, ovulation, corpus luteum formation, and ovulatory wound repair. Little is known about the cell state dynamics of the ovary during the estrous cycle and the paracrine factors that help coordinate this process. Herein, we used single-cell RNA sequencing to evaluate the transcriptome of >34,000 cells of the adult mouse ovary and describe the transcriptional changes that occur across the normal estrous cycle and other reproductive states to build a comprehensive dynamic atlas of murine ovarian cell types and states. |
| first_indexed | 2024-04-11T10:17:55Z |
| format | Article |
| id | doaj.art-0bb2b996f24446319cd33302421be382 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-11T10:17:55Z |
| publishDate | 2022-10-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-0bb2b996f24446319cd33302421be3822022-12-22T04:29:53ZengeLife Sciences Publications LtdeLife2050-084X2022-10-011110.7554/eLife.77239A single-cell atlas of the cycling murine ovaryMary E Morris0Marie-Charlotte Meinsohn1https://orcid.org/0000-0002-1378-4655Maeva Chauvin2Hatice D Saatcioglu3Aki Kashiwagi4Natalie A Sicher5Ngoc Nguyen6Selena Yuan7Rhian Stavely8Minsuk Hyun9Patricia K Donahoe10Bernardo L Sabatini11https://orcid.org/0000-0003-0095-9177David Pépin12https://orcid.org/0000-0003-2046-6708Department of Gynecology and Reproductive Biology, Massachusetts General Hospital, Boston, United StatesPediatric Surgical Research Laboratories, Massachusetts General Hospital, Boston, United States; Department of Surgery, Harvard Medical School, Boston, United StatesPediatric Surgical Research Laboratories, Massachusetts General Hospital, Boston, United States; Department of Surgery, Harvard Medical School, Boston, United StatesPediatric Surgical Research Laboratories, Massachusetts General Hospital, Boston, United States; Department of Surgery, Harvard Medical School, Boston, United StatesPediatric Surgical Research Laboratories, Massachusetts General Hospital, Boston, United States; Department of Surgery, Harvard Medical School, Boston, United StatesPediatric Surgical Research Laboratories, Massachusetts General Hospital, Boston, United States; Department of Surgery, Harvard Medical School, Boston, United StatesPediatric Surgical Research Laboratories, Massachusetts General Hospital, Boston, United States; Department of Surgery, Harvard Medical School, Boston, United StatesPediatric Surgical Research Laboratories, Massachusetts General Hospital, Boston, United States; Department of Surgery, Harvard Medical School, Boston, United StatesPediatric Surgical Research Laboratories, Massachusetts General Hospital, Boston, United States; Department of Surgery, Harvard Medical School, Boston, United StatesHoward Hughes Medical Institute, Department of Neurobiology, Harvard Medical School, Boston, United StatesPediatric Surgical Research Laboratories, Massachusetts General Hospital, Boston, United States; Department of Surgery, Harvard Medical School, Boston, United StatesHoward Hughes Medical Institute, Department of Neurobiology, Harvard Medical School, Boston, United StatesPediatric Surgical Research Laboratories, Massachusetts General Hospital, Boston, United States; Department of Surgery, Harvard Medical School, Boston, United StatesThe estrous cycle is regulated by rhythmic endocrine interactions of the nervous and reproductive systems, which coordinate the hormonal and ovulatory functions of the ovary. Folliculogenesis and follicle progression require the orchestrated response of a variety of cell types to allow the maturation of the follicle and its sequela, ovulation, corpus luteum formation, and ovulatory wound repair. Little is known about the cell state dynamics of the ovary during the estrous cycle and the paracrine factors that help coordinate this process. Herein, we used single-cell RNA sequencing to evaluate the transcriptome of >34,000 cells of the adult mouse ovary and describe the transcriptional changes that occur across the normal estrous cycle and other reproductive states to build a comprehensive dynamic atlas of murine ovarian cell types and states.https://elifesciences.org/articles/77239estrous cycleovarymousesingle-cell RNA sequencing |
| spellingShingle | Mary E Morris Marie-Charlotte Meinsohn Maeva Chauvin Hatice D Saatcioglu Aki Kashiwagi Natalie A Sicher Ngoc Nguyen Selena Yuan Rhian Stavely Minsuk Hyun Patricia K Donahoe Bernardo L Sabatini David Pépin A single-cell atlas of the cycling murine ovary eLife estrous cycle ovary mouse single-cell RNA sequencing |
| title | A single-cell atlas of the cycling murine ovary |
| title_full | A single-cell atlas of the cycling murine ovary |
| title_fullStr | A single-cell atlas of the cycling murine ovary |
| title_full_unstemmed | A single-cell atlas of the cycling murine ovary |
| title_short | A single-cell atlas of the cycling murine ovary |
| title_sort | single cell atlas of the cycling murine ovary |
| topic | estrous cycle ovary mouse single-cell RNA sequencing |
| url | https://elifesciences.org/articles/77239 |
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