First molecular identification of Euphlyctis ehrenbergii (Anura: Amphibia) inhabiting southwestern Saudi Arabia
The dicroglossid Euphlyctis ehrenbergii inhabiting southwestern Saudi Arabia has been identified morphologically and molecularly in this study. The morphometric measurements and their indices indicate that females are slightly bigger than males in some characters. Approximately 550 nucleotides of 16...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2019-01-01
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| Series: | The European Zoological Journal |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/24750263.2019.1609104 |
| _version_ | 1818947272204877824 |
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| author | A. R. Al-Qahtani S. A. M. Amer |
| author_facet | A. R. Al-Qahtani S. A. M. Amer |
| author_sort | A. R. Al-Qahtani |
| collection | DOAJ |
| description | The dicroglossid Euphlyctis ehrenbergii inhabiting southwestern Saudi Arabia has been identified morphologically and molecularly in this study. The morphometric measurements and their indices indicate that females are slightly bigger than males in some characters. Approximately 550 nucleotides of 16S rDNA gene were compared to the Yemeni haplotype available in the GenBank database. The phylogenetic inference revealed a strong sister relationship between the two haplotypes [bootstrap = 100% for maximum parsimony (MP), neighbor joining (NJ) and maximum likelihood (ML)]. The intra-specific genetic distance (D = 0.9%) was lower than the observed variation between southern and northern haplotypes of E. cyanophlyctis (D = 4.6%) currently ascribed to distinct lineages. Nonetheless, results suggest great intra-specific variation within E. ehrenbergii, deserving further investigation. The genetic distance between Yemeni and Saudi haplotypes of E. ehrenbergii (D = 0.9%) resembles intra-specific distances between haplotypes of E. cyanophlyctis ascribed to the same cryptic lineages (e.g. as observed within the lineages including haplotypes from southern India and Sri Lanka). More samples and molecular data from both haplotypes are needed to better clarify their phylogenetic relationships. This study adds a clue for forensic herpetology by highlighting the efficiency of 16S rDNA gene in animal barcoding. |
| first_indexed | 2024-12-20T08:28:16Z |
| format | Article |
| id | doaj.art-0bb43955e371428489d94cebdfb0b6f8 |
| institution | Directory Open Access Journal |
| issn | 2475-0263 |
| language | English |
| last_indexed | 2024-12-20T08:28:16Z |
| publishDate | 2019-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | The European Zoological Journal |
| spelling | doaj.art-0bb43955e371428489d94cebdfb0b6f82022-12-21T19:46:46ZengTaylor & Francis GroupThe European Zoological Journal2475-02632019-01-0186117317910.1080/24750263.2019.16091041609104First molecular identification of Euphlyctis ehrenbergii (Anura: Amphibia) inhabiting southwestern Saudi ArabiaA. R. Al-Qahtani0S. A. M. Amer1University of BishaCairo UniversityThe dicroglossid Euphlyctis ehrenbergii inhabiting southwestern Saudi Arabia has been identified morphologically and molecularly in this study. The morphometric measurements and their indices indicate that females are slightly bigger than males in some characters. Approximately 550 nucleotides of 16S rDNA gene were compared to the Yemeni haplotype available in the GenBank database. The phylogenetic inference revealed a strong sister relationship between the two haplotypes [bootstrap = 100% for maximum parsimony (MP), neighbor joining (NJ) and maximum likelihood (ML)]. The intra-specific genetic distance (D = 0.9%) was lower than the observed variation between southern and northern haplotypes of E. cyanophlyctis (D = 4.6%) currently ascribed to distinct lineages. Nonetheless, results suggest great intra-specific variation within E. ehrenbergii, deserving further investigation. The genetic distance between Yemeni and Saudi haplotypes of E. ehrenbergii (D = 0.9%) resembles intra-specific distances between haplotypes of E. cyanophlyctis ascribed to the same cryptic lineages (e.g. as observed within the lineages including haplotypes from southern India and Sri Lanka). More samples and molecular data from both haplotypes are needed to better clarify their phylogenetic relationships. This study adds a clue for forensic herpetology by highlighting the efficiency of 16S rDNA gene in animal barcoding.http://dx.doi.org/10.1080/24750263.2019.1609104ArabiaherpetologyDicroglossidaemtDNAphylogeny |
| spellingShingle | A. R. Al-Qahtani S. A. M. Amer First molecular identification of Euphlyctis ehrenbergii (Anura: Amphibia) inhabiting southwestern Saudi Arabia The European Zoological Journal Arabia herpetology Dicroglossidae mtDNA phylogeny |
| title | First molecular identification of Euphlyctis ehrenbergii (Anura: Amphibia) inhabiting southwestern Saudi Arabia |
| title_full | First molecular identification of Euphlyctis ehrenbergii (Anura: Amphibia) inhabiting southwestern Saudi Arabia |
| title_fullStr | First molecular identification of Euphlyctis ehrenbergii (Anura: Amphibia) inhabiting southwestern Saudi Arabia |
| title_full_unstemmed | First molecular identification of Euphlyctis ehrenbergii (Anura: Amphibia) inhabiting southwestern Saudi Arabia |
| title_short | First molecular identification of Euphlyctis ehrenbergii (Anura: Amphibia) inhabiting southwestern Saudi Arabia |
| title_sort | first molecular identification of euphlyctis ehrenbergii anura amphibia inhabiting southwestern saudi arabia |
| topic | Arabia herpetology Dicroglossidae mtDNA phylogeny |
| url | http://dx.doi.org/10.1080/24750263.2019.1609104 |
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