Molecular and in vivo Characterization of an Iranian Infectious Bursal Disease Virus Containing a Mixed Virus Population
BACKGROUND: Infectious bursal disease (IBD) is a highly contagious immunosuppressive disease of young chickens caused by infectious bursal disease virus (IBDV). IBDV consists of a two-segmented double-stranded RNA genome which can easily undergo genomic recombination or reassortment during mixed inf...
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| Format: | Article |
| Language: | English |
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University of Tehran
1999-11-01
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| Series: | Iranian Journal of Veterinary Medicine |
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| Online Access: | https://ijvm.ut.ac.ir/article_82238_63f39e0816fb4e7e1133af686812fa31.pdf |
| _version_ | 1819095306433724416 |
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| author | Amir Ghorbani Seyed Mostafa Peighambari Jamshid Razmyar |
| author_facet | Amir Ghorbani Seyed Mostafa Peighambari Jamshid Razmyar |
| author_sort | Amir Ghorbani |
| collection | DOAJ |
| description | BACKGROUND: Infectious bursal disease (IBD) is a highly contagious immunosuppressive disease of young chickens caused by infectious bursal disease virus (IBDV). IBDV consists of a two-segmented double-stranded RNA genome which can easily undergo genomic recombination or reassortment during mixed infections. OBJECTIVES:The objectives of this study were aimed to characterize a previously identified Iranian IBDV strain (JRMP29IR) in specific-pathogen-free (SPF) chickens, evaluate the presence of a mixed and/or reassortant virus popula-tion in this strain, and examine the frequency of genomic recombination and reassortment in publicly available IBDV genomes through bioinformatics study. METHODS:The SPF chickens were challenged with the JRMP29IR strain via oral and intraocular routes. Bursal tissues were extracted for histopathological evaluation and reverse transcription-polymerase chain reaction (RT-PCR) followed by Sanger sequencing. Putative recombinations and reassortments were evaluated using the Recombination Detection Program 5. RESULTS:Through genomic sequencing of the viruses from the bursas of infected chickens, the JRMP29IR strain was found which contained viruses from the classic, variant and very virulent IBDV genotypes. Through bioinformatics, nu-merous putative recombination and reassortment events were identified that naturally occur throughout the IBDV genome. CONCLUSIONS:Parental JRMP29IR appears to be derived from a flock undergoing a mixed IBDV infection. High frequency of recombination and reassortment among IBDVs suggests that these events are evolutionarily beneficial for the virus |
| first_indexed | 2024-12-21T23:41:12Z |
| format | Article |
| id | doaj.art-0bb624b386ce4647aa853ed676d216d0 |
| institution | Directory Open Access Journal |
| issn | 2251-8894 2252-0554 |
| language | English |
| last_indexed | 2024-12-21T23:41:12Z |
| publishDate | 1999-11-01 |
| publisher | University of Tehran |
| record_format | Article |
| series | Iranian Journal of Veterinary Medicine |
| spelling | doaj.art-0bb624b386ce4647aa853ed676d216d02022-12-21T18:46:13ZengUniversity of TehranIranian Journal of Veterinary Medicine2251-88942252-05541999-11-0115215516710.22059/ijvm.2020.305989.100510882238Molecular and in vivo Characterization of an Iranian Infectious Bursal Disease Virus Containing a Mixed Virus PopulationAmir Ghorbani0Seyed Mostafa Peighambari1Jamshid Razmyar2Department of Avian Diseases, Faculty of Veterinary Medicine, University of Tehran, Tehran, IranDepartment of Avian Diseases Faculty of Veterinary Medicine University of Tehran Tehran, IranDepartment of Avian Diseases, Faculty of Veterinary Medicine, University of Tehran, Tehran, IranBACKGROUND: Infectious bursal disease (IBD) is a highly contagious immunosuppressive disease of young chickens caused by infectious bursal disease virus (IBDV). IBDV consists of a two-segmented double-stranded RNA genome which can easily undergo genomic recombination or reassortment during mixed infections. OBJECTIVES:The objectives of this study were aimed to characterize a previously identified Iranian IBDV strain (JRMP29IR) in specific-pathogen-free (SPF) chickens, evaluate the presence of a mixed and/or reassortant virus popula-tion in this strain, and examine the frequency of genomic recombination and reassortment in publicly available IBDV genomes through bioinformatics study. METHODS:The SPF chickens were challenged with the JRMP29IR strain via oral and intraocular routes. Bursal tissues were extracted for histopathological evaluation and reverse transcription-polymerase chain reaction (RT-PCR) followed by Sanger sequencing. Putative recombinations and reassortments were evaluated using the Recombination Detection Program 5. RESULTS:Through genomic sequencing of the viruses from the bursas of infected chickens, the JRMP29IR strain was found which contained viruses from the classic, variant and very virulent IBDV genotypes. Through bioinformatics, nu-merous putative recombination and reassortment events were identified that naturally occur throughout the IBDV genome. CONCLUSIONS:Parental JRMP29IR appears to be derived from a flock undergoing a mixed IBDV infection. High frequency of recombination and reassortment among IBDVs suggests that these events are evolutionarily beneficial for the virushttps://ijvm.ut.ac.ir/article_82238_63f39e0816fb4e7e1133af686812fa31.pdfbursal diseasemixed virusimmunosuppressive |
| spellingShingle | Amir Ghorbani Seyed Mostafa Peighambari Jamshid Razmyar Molecular and in vivo Characterization of an Iranian Infectious Bursal Disease Virus Containing a Mixed Virus Population Iranian Journal of Veterinary Medicine bursal disease mixed virus immunosuppressive |
| title | Molecular and in vivo Characterization of an Iranian Infectious Bursal Disease Virus Containing a Mixed Virus Population |
| title_full | Molecular and in vivo Characterization of an Iranian Infectious Bursal Disease Virus Containing a Mixed Virus Population |
| title_fullStr | Molecular and in vivo Characterization of an Iranian Infectious Bursal Disease Virus Containing a Mixed Virus Population |
| title_full_unstemmed | Molecular and in vivo Characterization of an Iranian Infectious Bursal Disease Virus Containing a Mixed Virus Population |
| title_short | Molecular and in vivo Characterization of an Iranian Infectious Bursal Disease Virus Containing a Mixed Virus Population |
| title_sort | molecular and in vivo characterization of an iranian infectious bursal disease virus containing a mixed virus population |
| topic | bursal disease mixed virus immunosuppressive |
| url | https://ijvm.ut.ac.ir/article_82238_63f39e0816fb4e7e1133af686812fa31.pdf |
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