Tamarind shell extract mitigates D-glucose-induced eye malformations: Restorative actions on key developmental markers in chick embryos and ARPE-19 cells
Eye deformity, a noteworthy embryopathy, is intricately linked with maternal diabetes, frequently resulting in embryonic malformation or even death. In this investigation, a hyperglycemic chick embryo model was established to delve into the protective potential of tamarind shell extract (TSE) on eye...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-04-01
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| Series: | Journal of Functional Foods |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464624001026 |
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| author | Xi-you Li Xiao-yuan Gu Bai-ling Zhou Bun Tsoi Wei-xi Li |
| author_facet | Xi-you Li Xiao-yuan Gu Bai-ling Zhou Bun Tsoi Wei-xi Li |
| author_sort | Xi-you Li |
| collection | DOAJ |
| description | Eye deformity, a noteworthy embryopathy, is intricately linked with maternal diabetes, frequently resulting in embryonic malformation or even death. In this investigation, a hyperglycemic chick embryo model was established to delve into the protective potential of tamarind shell extract (TSE) on eye development. Chick embryos were administered with TSE (at dosages of 1, 2, and 4 µg/egg) through air sac injection on embryo development day 0 (EDD0) and later exposed to D-glucose (0.4 mmol/egg) on EDD1. To validate our findings, we replicated hyperglycemic environment using D-glucose in ARPE-19 cell model. Our research unveiled that TSE proficiently counters O-GlcNAcylation of proteins in the presence of D-glucose, thereby reinstating levels of key proteins involved in eye development, including Pax6, Daam1, mTOR, and Cdc42. Furthermore, TSE was pinpointed to curtail the D-glucose concentration within the embryo eye, considerably decreasing the accumulation of reactive oxygen species and malondialdehyde accumulation in both the ARPE-19 cells and chick embryos. Beyond its fundamental role in boosting the cellular antioxidative activity, the improved expression of apoptosis-related proteins like Caspase-3, BAX, BCL-2 and other like PI3K and AKT were observed. This suggests a probable activation of the PI3K-AKT-mTOR pathway on ARPE-19 cells by TSE, thereby downregulating apoptotic proteins in ARPE-19 cells during oxidative stress. TSE may become a potent natural countermeasure against D-glucose-induced eye malformation. |
| first_indexed | 2024-04-24T22:20:59Z |
| format | Article |
| id | doaj.art-0bb78eaf7bbd4271b99e4766c21e5ec6 |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-04-24T22:20:59Z |
| publishDate | 2024-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-0bb78eaf7bbd4271b99e4766c21e5ec62024-03-20T06:09:04ZengElsevierJournal of Functional Foods1756-46462024-04-01115106100Tamarind shell extract mitigates D-glucose-induced eye malformations: Restorative actions on key developmental markers in chick embryos and ARPE-19 cellsXi-you Li0Xiao-yuan Gu1Bai-ling Zhou2Bun Tsoi3Wei-xi Li4College of Traditional Chinese Medicine, Yunnan University of Chinese Medicine, Kunming 650500, ChinaCollege of Traditional Chinese Medicine, Yunnan University of Chinese Medicine, Kunming 650500, ChinaDepartment of Laboratory Medicine, Kunming Children’s Hospital, Kunming 650034, ChinaDepartment of Food Science and Nutrition, The Hong Kong Polytechnic University, Hung Hum, Kowloon, Hong Kong Special Administrative Region; Research Centre for Chinese Medicine Innovation, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong Special Administrative Region; Corresponding authors at: Department of Food Science and Nutrition, The Hong Kong Polytechnic University, Hung Hum, Kowloon, Hong Kong Special Administrative Region (B. Tsoi).College of Traditional Chinese Medicine, Yunnan University of Chinese Medicine, Kunming 650500, China; Corresponding authors at: Department of Food Science and Nutrition, The Hong Kong Polytechnic University, Hung Hum, Kowloon, Hong Kong Special Administrative Region (B. Tsoi).Eye deformity, a noteworthy embryopathy, is intricately linked with maternal diabetes, frequently resulting in embryonic malformation or even death. In this investigation, a hyperglycemic chick embryo model was established to delve into the protective potential of tamarind shell extract (TSE) on eye development. Chick embryos were administered with TSE (at dosages of 1, 2, and 4 µg/egg) through air sac injection on embryo development day 0 (EDD0) and later exposed to D-glucose (0.4 mmol/egg) on EDD1. To validate our findings, we replicated hyperglycemic environment using D-glucose in ARPE-19 cell model. Our research unveiled that TSE proficiently counters O-GlcNAcylation of proteins in the presence of D-glucose, thereby reinstating levels of key proteins involved in eye development, including Pax6, Daam1, mTOR, and Cdc42. Furthermore, TSE was pinpointed to curtail the D-glucose concentration within the embryo eye, considerably decreasing the accumulation of reactive oxygen species and malondialdehyde accumulation in both the ARPE-19 cells and chick embryos. Beyond its fundamental role in boosting the cellular antioxidative activity, the improved expression of apoptosis-related proteins like Caspase-3, BAX, BCL-2 and other like PI3K and AKT were observed. This suggests a probable activation of the PI3K-AKT-mTOR pathway on ARPE-19 cells by TSE, thereby downregulating apoptotic proteins in ARPE-19 cells during oxidative stress. TSE may become a potent natural countermeasure against D-glucose-induced eye malformation.http://www.sciencedirect.com/science/article/pii/S1756464624001026Tamarind shell extractEye malformationOxidative stressApoptosisChick embryoARPE-19 |
| spellingShingle | Xi-you Li Xiao-yuan Gu Bai-ling Zhou Bun Tsoi Wei-xi Li Tamarind shell extract mitigates D-glucose-induced eye malformations: Restorative actions on key developmental markers in chick embryos and ARPE-19 cells Journal of Functional Foods Tamarind shell extract Eye malformation Oxidative stress Apoptosis Chick embryo ARPE-19 |
| title | Tamarind shell extract mitigates D-glucose-induced eye malformations: Restorative actions on key developmental markers in chick embryos and ARPE-19 cells |
| title_full | Tamarind shell extract mitigates D-glucose-induced eye malformations: Restorative actions on key developmental markers in chick embryos and ARPE-19 cells |
| title_fullStr | Tamarind shell extract mitigates D-glucose-induced eye malformations: Restorative actions on key developmental markers in chick embryos and ARPE-19 cells |
| title_full_unstemmed | Tamarind shell extract mitigates D-glucose-induced eye malformations: Restorative actions on key developmental markers in chick embryos and ARPE-19 cells |
| title_short | Tamarind shell extract mitigates D-glucose-induced eye malformations: Restorative actions on key developmental markers in chick embryos and ARPE-19 cells |
| title_sort | tamarind shell extract mitigates d glucose induced eye malformations restorative actions on key developmental markers in chick embryos and arpe 19 cells |
| topic | Tamarind shell extract Eye malformation Oxidative stress Apoptosis Chick embryo ARPE-19 |
| url | http://www.sciencedirect.com/science/article/pii/S1756464624001026 |
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