Chromosome constitution and genetic relationships of Morus spp. revealed by genomic in situ hybridization
Abstract Background Mulberry (Morus spp.) is an economically important woody plant, which has been used for sericulture (silk farming) for thousands of years. The genetic background of mulberry is complex due to polyploidy and frequent hybridization events. Results Comparative genomic in situ hybrid...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-09-01
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| Series: | BMC Plant Biology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12870-023-04448-9 |
| _version_ | 1797453317800984576 |
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| author | Yahui Xuan Sheng Wang Siwei Li Jianglian Yuan Qiming Zhou Ningjia He |
| author_facet | Yahui Xuan Sheng Wang Siwei Li Jianglian Yuan Qiming Zhou Ningjia He |
| author_sort | Yahui Xuan |
| collection | DOAJ |
| description | Abstract Background Mulberry (Morus spp.) is an economically important woody plant, which has been used for sericulture (silk farming) for thousands of years. The genetic background of mulberry is complex due to polyploidy and frequent hybridization events. Results Comparative genomic in situ hybridization (cGISH) and self-GISH were performed to illustrate the chromosome constitution and genetic relationships of 40 mulberry accessions belonging to 12 species and three varietas in the Morus genus and containing eight different ploidy levels. We identified six homozygous cGISH signal patterns and one heterozygous cGISH signal pattern using four genomic DNA probes. Using cGISH and self-GISH data, we defined five mulberry sections (Notabilis, Nigra, Wittiorum, and Cathayana, all contained only one species; and Alba, which contained seven closely related species and three varietas, was further divided into two subsections) and proposed the genetic relationships among them. Differential cGISH signal patterns detected in section Alba allowed us to refine the genetic relationships among the closely related members of this section. Conclusions We propose that GISH is an efficient tool to investigate the chromosome constitution and genetic relationships in mulberry. The results obtained here can be used to guide outbreeding of heterozygous perennial crops like mulberry. |
| first_indexed | 2024-03-09T15:21:05Z |
| format | Article |
| id | doaj.art-0bbcebc7af33492bb6ae28ccb14f2fd9 |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-03-09T15:21:05Z |
| publishDate | 2023-09-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-0bbcebc7af33492bb6ae28ccb14f2fd92023-11-26T12:46:49ZengBMCBMC Plant Biology1471-22292023-09-0123111610.1186/s12870-023-04448-9Chromosome constitution and genetic relationships of Morus spp. revealed by genomic in situ hybridizationYahui Xuan0Sheng Wang1Siwei Li2Jianglian Yuan3Qiming Zhou4Ningjia He5State Key Laboratory of Resource Insects, Southwest UniversityState Key Laboratory of Resource Insects, Southwest UniversityState Key Laboratory of Resource Insects, Southwest UniversityState Key Laboratory of Resource Insects, Southwest UniversityState Key Laboratory of Resource Insects, Southwest UniversityState Key Laboratory of Resource Insects, Southwest UniversityAbstract Background Mulberry (Morus spp.) is an economically important woody plant, which has been used for sericulture (silk farming) for thousands of years. The genetic background of mulberry is complex due to polyploidy and frequent hybridization events. Results Comparative genomic in situ hybridization (cGISH) and self-GISH were performed to illustrate the chromosome constitution and genetic relationships of 40 mulberry accessions belonging to 12 species and three varietas in the Morus genus and containing eight different ploidy levels. We identified six homozygous cGISH signal patterns and one heterozygous cGISH signal pattern using four genomic DNA probes. Using cGISH and self-GISH data, we defined five mulberry sections (Notabilis, Nigra, Wittiorum, and Cathayana, all contained only one species; and Alba, which contained seven closely related species and three varietas, was further divided into two subsections) and proposed the genetic relationships among them. Differential cGISH signal patterns detected in section Alba allowed us to refine the genetic relationships among the closely related members of this section. Conclusions We propose that GISH is an efficient tool to investigate the chromosome constitution and genetic relationships in mulberry. The results obtained here can be used to guide outbreeding of heterozygous perennial crops like mulberry.https://doi.org/10.1186/s12870-023-04448-9MorusGenomic in situ hybridizationChromosome constitutionGenetic relationshipsMulberry section |
| spellingShingle | Yahui Xuan Sheng Wang Siwei Li Jianglian Yuan Qiming Zhou Ningjia He Chromosome constitution and genetic relationships of Morus spp. revealed by genomic in situ hybridization BMC Plant Biology Morus Genomic in situ hybridization Chromosome constitution Genetic relationships Mulberry section |
| title | Chromosome constitution and genetic relationships of Morus spp. revealed by genomic in situ hybridization |
| title_full | Chromosome constitution and genetic relationships of Morus spp. revealed by genomic in situ hybridization |
| title_fullStr | Chromosome constitution and genetic relationships of Morus spp. revealed by genomic in situ hybridization |
| title_full_unstemmed | Chromosome constitution and genetic relationships of Morus spp. revealed by genomic in situ hybridization |
| title_short | Chromosome constitution and genetic relationships of Morus spp. revealed by genomic in situ hybridization |
| title_sort | chromosome constitution and genetic relationships of morus spp revealed by genomic in situ hybridization |
| topic | Morus Genomic in situ hybridization Chromosome constitution Genetic relationships Mulberry section |
| url | https://doi.org/10.1186/s12870-023-04448-9 |
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