dSir2 in the Adult Fat Body, but Not in Muscles, Regulates Life Span in a Diet-Dependent Manner
Sir2, an evolutionarily conserved NAD+-dependent deacetylase, has been implicated as a key factor in mediating organismal life span. However, recent contradictory findings have brought into question the role of Sir2 and its orthologs in regulating organismal longevity. In this study, we report that...
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| Format: | Article |
| Language: | English |
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Elsevier
2012-12-01
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| Series: | Cell Reports |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124712003944 |
| _version_ | 1818146204734717952 |
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| author | Kushal Kr. Banerjee Champakali Ayyub Syed Zeeshan Ali Vinesh Mandot Nagaraj G. Prasad Ullas Kolthur-Seetharam |
| author_facet | Kushal Kr. Banerjee Champakali Ayyub Syed Zeeshan Ali Vinesh Mandot Nagaraj G. Prasad Ullas Kolthur-Seetharam |
| author_sort | Kushal Kr. Banerjee |
| collection | DOAJ |
| description | Sir2, an evolutionarily conserved NAD+-dependent deacetylase, has been implicated as a key factor in mediating organismal life span. However, recent contradictory findings have brought into question the role of Sir2 and its orthologs in regulating organismal longevity. In this study, we report that Drosophila Sir2 (dSir2) in the adult fat body regulates longevity in a diet-dependent manner. We used inducible Gal4 drivers to knock down and overexpress dSir2 in a tissue-specific manner. A diet-dependent life span phenotype of dSir2 perturbations (both knockdown and overexpression) in the fat body, but not muscles, negates the effects of background genetic mutations. In addition to providing clarity to the field, our study contrasts the ability of dSir2 in two metabolic tissues to affect longevity. We also show that dSir2 knockdown abrogates fat-body dFOXO-dependent life span extension. This report highlights the importance of the interplay between genetic factors and dietary inputs in determining organismal life spans. |
| first_indexed | 2024-12-11T12:15:38Z |
| format | Article |
| id | doaj.art-0bbe236322e44c2d9ea048f30b8ebc57 |
| institution | Directory Open Access Journal |
| issn | 2211-1247 |
| language | English |
| last_indexed | 2024-12-11T12:15:38Z |
| publishDate | 2012-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj.art-0bbe236322e44c2d9ea048f30b8ebc572022-12-22T01:07:39ZengElsevierCell Reports2211-12472012-12-01261485149110.1016/j.celrep.2012.11.013dSir2 in the Adult Fat Body, but Not in Muscles, Regulates Life Span in a Diet-Dependent MannerKushal Kr. Banerjee0Champakali Ayyub1Syed Zeeshan Ali2Vinesh Mandot3Nagaraj G. Prasad4Ullas Kolthur-Seetharam5Department of Biological Sciences, Tata Institute of Fundamental Research, Homi Bhabha Road, Colaba, Mumbai 400005, IndiaDepartment of Biological Sciences, Tata Institute of Fundamental Research, Homi Bhabha Road, Colaba, Mumbai 400005, IndiaIndian Institute of Science Education and Research, Mohali, Sector 81, SAS Nagar, PO Manauli, Punjab 140306, IndiaDepartment of Biological Sciences, Tata Institute of Fundamental Research, Homi Bhabha Road, Colaba, Mumbai 400005, IndiaIndian Institute of Science Education and Research, Mohali, Sector 81, SAS Nagar, PO Manauli, Punjab 140306, IndiaDepartment of Biological Sciences, Tata Institute of Fundamental Research, Homi Bhabha Road, Colaba, Mumbai 400005, IndiaSir2, an evolutionarily conserved NAD+-dependent deacetylase, has been implicated as a key factor in mediating organismal life span. However, recent contradictory findings have brought into question the role of Sir2 and its orthologs in regulating organismal longevity. In this study, we report that Drosophila Sir2 (dSir2) in the adult fat body regulates longevity in a diet-dependent manner. We used inducible Gal4 drivers to knock down and overexpress dSir2 in a tissue-specific manner. A diet-dependent life span phenotype of dSir2 perturbations (both knockdown and overexpression) in the fat body, but not muscles, negates the effects of background genetic mutations. In addition to providing clarity to the field, our study contrasts the ability of dSir2 in two metabolic tissues to affect longevity. We also show that dSir2 knockdown abrogates fat-body dFOXO-dependent life span extension. This report highlights the importance of the interplay between genetic factors and dietary inputs in determining organismal life spans.http://www.sciencedirect.com/science/article/pii/S2211124712003944 |
| spellingShingle | Kushal Kr. Banerjee Champakali Ayyub Syed Zeeshan Ali Vinesh Mandot Nagaraj G. Prasad Ullas Kolthur-Seetharam dSir2 in the Adult Fat Body, but Not in Muscles, Regulates Life Span in a Diet-Dependent Manner Cell Reports |
| title | dSir2 in the Adult Fat Body, but Not in Muscles, Regulates Life Span in a Diet-Dependent Manner |
| title_full | dSir2 in the Adult Fat Body, but Not in Muscles, Regulates Life Span in a Diet-Dependent Manner |
| title_fullStr | dSir2 in the Adult Fat Body, but Not in Muscles, Regulates Life Span in a Diet-Dependent Manner |
| title_full_unstemmed | dSir2 in the Adult Fat Body, but Not in Muscles, Regulates Life Span in a Diet-Dependent Manner |
| title_short | dSir2 in the Adult Fat Body, but Not in Muscles, Regulates Life Span in a Diet-Dependent Manner |
| title_sort | dsir2 in the adult fat body but not in muscles regulates life span in a diet dependent manner |
| url | http://www.sciencedirect.com/science/article/pii/S2211124712003944 |
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