Influence of Equilibration Time and Bull-Specific Extender for Cryopreservation on Semen Quality and Fertility in German Holstein Friesian Bulls: A Controlled Field Trial
Equilibration with an extender is necessary to allow cryopreservation of bovine sperm. The aim of trial 1 was to assess the effect of 24 h versus 4 h equilibration time with three different extenders on sperm quality and to select the preferred extender for each bull. The aim of trial 2 was to inves...
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| Format: | Article |
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MDPI AG
2023-07-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/13/14/2285 |
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| author | Laura Pieper Tristan Meschede Markus Jung Ulrich Janowitz Martin Schulze |
| author_facet | Laura Pieper Tristan Meschede Markus Jung Ulrich Janowitz Martin Schulze |
| author_sort | Laura Pieper |
| collection | DOAJ |
| description | Equilibration with an extender is necessary to allow cryopreservation of bovine sperm. The aim of trial 1 was to assess the effect of 24 h versus 4 h equilibration time with three different extenders on sperm quality and to select the preferred extender for each bull. The aim of trial 2 was to investigate the effect of using a 24 h equilibration time with a bull-specific extender on field fertility. For trial 1, three ejaculates each from eight Holstein Friesian breeding bulls were used as the split-sample, including two equilibration times (4 h and 24 h) and three extenders (BioXcell, Triladyl, and OptiXcell). For trial 2, from 5 to 10 ejaculates from the same bulls were collected and treated (split-sample) as BioXcell with 4 h equilibration and either Triladyl or OptiXcell, both with 24 h equilibration. A total of 11,059 straws were used for insemination of cows and heifers. For Triladyl, progressive sperm motility, acrosome defects, and plasma membrane and acrosome integrity improved with a 24 h compared to a 4 h equilibration time. Four bulls each were used with Triladyl and OptiXcell for trial 2. In trial 2, non-return rates did not differ among groups. Therefore, using a 24 h equilibration time might improve in vitro sperm parameters, depending on the extender used. Moreover, it would be possible to change from 4 h to 24 h equilibration time without impairing field fertility. |
| first_indexed | 2024-03-11T01:22:11Z |
| format | Article |
| id | doaj.art-0bbee0f2f08949a69908be604268a04e |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-11T01:22:11Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-0bbee0f2f08949a69908be604268a04e2023-11-18T18:00:09ZengMDPI AGAnimals2076-26152023-07-011314228510.3390/ani13142285Influence of Equilibration Time and Bull-Specific Extender for Cryopreservation on Semen Quality and Fertility in German Holstein Friesian Bulls: A Controlled Field TrialLaura Pieper0Tristan Meschede1Markus Jung2Ulrich Janowitz3Martin Schulze4Institute for Reproduction of Farm Animals Schönow, Bernauer Allee 10, D-16321 Bernau, GermanyRinder-Union West eG, Schifffahrter Damm 235a, D-48147 Muenster, GermanyInstitute for Reproduction of Farm Animals Schönow, Bernauer Allee 10, D-16321 Bernau, GermanyRinder-Union West eG, Schifffahrter Damm 235a, D-48147 Muenster, GermanyInstitute for Reproduction of Farm Animals Schönow, Bernauer Allee 10, D-16321 Bernau, GermanyEquilibration with an extender is necessary to allow cryopreservation of bovine sperm. The aim of trial 1 was to assess the effect of 24 h versus 4 h equilibration time with three different extenders on sperm quality and to select the preferred extender for each bull. The aim of trial 2 was to investigate the effect of using a 24 h equilibration time with a bull-specific extender on field fertility. For trial 1, three ejaculates each from eight Holstein Friesian breeding bulls were used as the split-sample, including two equilibration times (4 h and 24 h) and three extenders (BioXcell, Triladyl, and OptiXcell). For trial 2, from 5 to 10 ejaculates from the same bulls were collected and treated (split-sample) as BioXcell with 4 h equilibration and either Triladyl or OptiXcell, both with 24 h equilibration. A total of 11,059 straws were used for insemination of cows and heifers. For Triladyl, progressive sperm motility, acrosome defects, and plasma membrane and acrosome integrity improved with a 24 h compared to a 4 h equilibration time. Four bulls each were used with Triladyl and OptiXcell for trial 2. In trial 2, non-return rates did not differ among groups. Therefore, using a 24 h equilibration time might improve in vitro sperm parameters, depending on the extender used. Moreover, it would be possible to change from 4 h to 24 h equilibration time without impairing field fertility.https://www.mdpi.com/2076-2615/13/14/2285bovineequilibration timecryopreservationextenderfertilitysperm quality |
| spellingShingle | Laura Pieper Tristan Meschede Markus Jung Ulrich Janowitz Martin Schulze Influence of Equilibration Time and Bull-Specific Extender for Cryopreservation on Semen Quality and Fertility in German Holstein Friesian Bulls: A Controlled Field Trial Animals bovine equilibration time cryopreservation extender fertility sperm quality |
| title | Influence of Equilibration Time and Bull-Specific Extender for Cryopreservation on Semen Quality and Fertility in German Holstein Friesian Bulls: A Controlled Field Trial |
| title_full | Influence of Equilibration Time and Bull-Specific Extender for Cryopreservation on Semen Quality and Fertility in German Holstein Friesian Bulls: A Controlled Field Trial |
| title_fullStr | Influence of Equilibration Time and Bull-Specific Extender for Cryopreservation on Semen Quality and Fertility in German Holstein Friesian Bulls: A Controlled Field Trial |
| title_full_unstemmed | Influence of Equilibration Time and Bull-Specific Extender for Cryopreservation on Semen Quality and Fertility in German Holstein Friesian Bulls: A Controlled Field Trial |
| title_short | Influence of Equilibration Time and Bull-Specific Extender for Cryopreservation on Semen Quality and Fertility in German Holstein Friesian Bulls: A Controlled Field Trial |
| title_sort | influence of equilibration time and bull specific extender for cryopreservation on semen quality and fertility in german holstein friesian bulls a controlled field trial |
| topic | bovine equilibration time cryopreservation extender fertility sperm quality |
| url | https://www.mdpi.com/2076-2615/13/14/2285 |
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