<i>Peucedanum japonicum</i> Thunberg and Its Active Components Mitigate Oxidative Stress, Inflammation and Apoptosis after Urban Particulate Matter-Induced Ocular Surface Damage
We previously demonstrated that urban particulate matter (UPM) exposure decreases the migration activity and survival of human corneal epithelial cells (HCECs). Herein, we investigated the potential to improve the corneal wound-healing ability of <i>Peucedanum japonicum</i> Thunb. leaf e...
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MDPI AG
2021-10-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/10/11/1717 |
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| author | Wan Seok Kang Hakjoon Choi Ki Hoon Lee Eun Kim Kyeong Jo Kim Jin Seok Kim Chang-Su Na Sunoh Kim |
| author_facet | Wan Seok Kang Hakjoon Choi Ki Hoon Lee Eun Kim Kyeong Jo Kim Jin Seok Kim Chang-Su Na Sunoh Kim |
| author_sort | Wan Seok Kang |
| collection | DOAJ |
| description | We previously demonstrated that urban particulate matter (UPM) exposure decreases the migration activity and survival of human corneal epithelial cells (HCECs). Herein, we investigated the potential to improve the corneal wound-healing ability of <i>Peucedanum japonicum</i> Thunb. leaf extract (PJE) and its active components on UPM-induced ocular surface damage in vitro and in vivo. PJE effectively assisted wound healing without altering HCEC survival and enhanced catalase (CAT), heme oxygenase 1 (HO1) and glutathione peroxidase 1 (GPX1) antioxidant gene expression. A corneal wound was uniformly induced on the right eye in all experimental animals and divided into eight groups such as two control groups (wounded right eye group—NR and non-wounded left eye group—NL), UPM treated group and PJEs (25, 50, 100, 200, 400 mg/kg) treated groups. Corneal abrasion model rats exposed to UPM showed delayed wound healing compared to unexposed rats, but wound healing was dose-dependently enhanced by PJE oral administration. Seventy-two hours after wound generation, inflammatory cells, apoptotic cells and interleukin-6 (IL-6) expression were increased substantially after UPM exposure, but PJE treatment significantly reduced the wound to an almost normal level while enhancing re-epithelialization without changing corneal thickness. Next, we tried to identify the key molecules for enhancing wound healing through fractionation. The major compounds in the fraction, confirmed by high-performance liquid chromatography (HPLC), were chlorogenic acid (CA), neochlorogenic acid (NCA) and cryptochlorogenic acid (CCA). Each type of CA isomers showed slightly different half maximal effective (EC50) and maximal effective (ECmax) concentrations, and their mixtures synergistically enhanced HCEC migration. Thus, corneal abrasion wound recovery after UPM exposure improved after PJE treatment, and the active PJE components were identified, providing an important basis to develop therapeutics for ocular surface damage using PJE. |
| first_indexed | 2024-03-10T05:44:47Z |
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| id | doaj.art-0bc0e822718e4e1aa830f04b3c6453d8 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-10T05:44:47Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-0bc0e822718e4e1aa830f04b3c6453d82023-11-22T22:12:39ZengMDPI AGAntioxidants2076-39212021-10-011011171710.3390/antiox10111717<i>Peucedanum japonicum</i> Thunberg and Its Active Components Mitigate Oxidative Stress, Inflammation and Apoptosis after Urban Particulate Matter-Induced Ocular Surface DamageWan Seok Kang0Hakjoon Choi1Ki Hoon Lee2Eun Kim3Kyeong Jo Kim4Jin Seok Kim5Chang-Su Na6Sunoh Kim7Central R&D Center, B&Tech Co., Ltd., Gwangju 61239, KoreaCentral R&D Center, B&Tech Co., Ltd., Gwangju 61239, KoreaCentral R&D Center, B&Tech Co., Ltd., Gwangju 61239, KoreaCentral R&D Center, B&Tech Co., Ltd., Gwangju 61239, KoreaCentral R&D Center, B&Tech Co., Ltd., Gwangju 61239, KoreaCentral R&D Center, B&Tech Co., Ltd., Gwangju 61239, KoreaCollege of Korean Medicine, Dongshin University, Naju-si 58245, KoreaCentral R&D Center, B&Tech Co., Ltd., Gwangju 61239, KoreaWe previously demonstrated that urban particulate matter (UPM) exposure decreases the migration activity and survival of human corneal epithelial cells (HCECs). Herein, we investigated the potential to improve the corneal wound-healing ability of <i>Peucedanum japonicum</i> Thunb. leaf extract (PJE) and its active components on UPM-induced ocular surface damage in vitro and in vivo. PJE effectively assisted wound healing without altering HCEC survival and enhanced catalase (CAT), heme oxygenase 1 (HO1) and glutathione peroxidase 1 (GPX1) antioxidant gene expression. A corneal wound was uniformly induced on the right eye in all experimental animals and divided into eight groups such as two control groups (wounded right eye group—NR and non-wounded left eye group—NL), UPM treated group and PJEs (25, 50, 100, 200, 400 mg/kg) treated groups. Corneal abrasion model rats exposed to UPM showed delayed wound healing compared to unexposed rats, but wound healing was dose-dependently enhanced by PJE oral administration. Seventy-two hours after wound generation, inflammatory cells, apoptotic cells and interleukin-6 (IL-6) expression were increased substantially after UPM exposure, but PJE treatment significantly reduced the wound to an almost normal level while enhancing re-epithelialization without changing corneal thickness. Next, we tried to identify the key molecules for enhancing wound healing through fractionation. The major compounds in the fraction, confirmed by high-performance liquid chromatography (HPLC), were chlorogenic acid (CA), neochlorogenic acid (NCA) and cryptochlorogenic acid (CCA). Each type of CA isomers showed slightly different half maximal effective (EC50) and maximal effective (ECmax) concentrations, and their mixtures synergistically enhanced HCEC migration. Thus, corneal abrasion wound recovery after UPM exposure improved after PJE treatment, and the active PJE components were identified, providing an important basis to develop therapeutics for ocular surface damage using PJE.https://www.mdpi.com/2076-3921/10/11/1717urban particulate matter (UPM)<i>Peucedanum japonicum</i> Thunbergcorneal epithelial cellswound healingantioxidantinflammation |
| spellingShingle | Wan Seok Kang Hakjoon Choi Ki Hoon Lee Eun Kim Kyeong Jo Kim Jin Seok Kim Chang-Su Na Sunoh Kim <i>Peucedanum japonicum</i> Thunberg and Its Active Components Mitigate Oxidative Stress, Inflammation and Apoptosis after Urban Particulate Matter-Induced Ocular Surface Damage Antioxidants urban particulate matter (UPM) <i>Peucedanum japonicum</i> Thunberg corneal epithelial cells wound healing antioxidant inflammation |
| title | <i>Peucedanum japonicum</i> Thunberg and Its Active Components Mitigate Oxidative Stress, Inflammation and Apoptosis after Urban Particulate Matter-Induced Ocular Surface Damage |
| title_full | <i>Peucedanum japonicum</i> Thunberg and Its Active Components Mitigate Oxidative Stress, Inflammation and Apoptosis after Urban Particulate Matter-Induced Ocular Surface Damage |
| title_fullStr | <i>Peucedanum japonicum</i> Thunberg and Its Active Components Mitigate Oxidative Stress, Inflammation and Apoptosis after Urban Particulate Matter-Induced Ocular Surface Damage |
| title_full_unstemmed | <i>Peucedanum japonicum</i> Thunberg and Its Active Components Mitigate Oxidative Stress, Inflammation and Apoptosis after Urban Particulate Matter-Induced Ocular Surface Damage |
| title_short | <i>Peucedanum japonicum</i> Thunberg and Its Active Components Mitigate Oxidative Stress, Inflammation and Apoptosis after Urban Particulate Matter-Induced Ocular Surface Damage |
| title_sort | i peucedanum japonicum i thunberg and its active components mitigate oxidative stress inflammation and apoptosis after urban particulate matter induced ocular surface damage |
| topic | urban particulate matter (UPM) <i>Peucedanum japonicum</i> Thunberg corneal epithelial cells wound healing antioxidant inflammation |
| url | https://www.mdpi.com/2076-3921/10/11/1717 |
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